##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633460.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	872867
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.910784804557853	33.0	33.0	33.0	33.0	33.0
2	32.022036576019026	33.0	33.0	33.0	33.0	33.0
3	31.740481654135166	33.0	33.0	33.0	27.0	33.0
4	32.019042992804174	33.0	33.0	33.0	33.0	33.0
5	32.120389475143405	33.0	33.0	33.0	33.0	33.0
6	35.29920251309764	37.0	37.0	37.0	33.0	37.0
7	35.435823556166056	37.0	37.0	37.0	33.0	37.0
8	35.56593959904544	37.0	37.0	37.0	33.0	37.0
9	35.679110334105886	37.0	37.0	37.0	33.0	37.0
10	35.69056568755607	37.0	37.0	37.0	33.0	37.0
11	35.71074745637079	37.0	37.0	37.0	33.0	37.0
12	35.7061476719821	37.0	37.0	37.0	33.0	37.0
13	35.70501347857119	37.0	37.0	37.0	33.0	37.0
14	35.67326981086465	37.0	37.0	37.0	33.0	37.0
15	35.69571652955147	37.0	37.0	37.0	33.0	37.0
16	35.67973815025657	37.0	37.0	37.0	33.0	37.0
17	35.664341761115956	37.0	37.0	37.0	33.0	37.0
18	35.65544464391483	37.0	37.0	37.0	33.0	37.0
19	35.63610492778396	37.0	37.0	37.0	33.0	37.0
20	35.6376779051104	37.0	37.0	37.0	33.0	37.0
21	35.645448848449995	37.0	37.0	37.0	33.0	37.0
22	35.55560354555734	37.0	37.0	37.0	33.0	37.0
23	35.597174598191934	37.0	37.0	37.0	33.0	37.0
24	35.58874376050418	37.0	37.0	37.0	33.0	37.0
25	35.61753623404253	37.0	37.0	37.0	33.0	37.0
26	35.49672172278251	37.0	37.0	37.0	33.0	37.0
27	35.512165083569435	37.0	37.0	37.0	33.0	37.0
28	35.53407678374827	37.0	37.0	37.0	33.0	37.0
29	35.53953924251919	37.0	37.0	37.0	33.0	37.0
30	35.55387361419323	37.0	37.0	37.0	33.0	37.0
31	35.537518316077936	37.0	37.0	37.0	33.0	37.0
32	35.53240642617948	37.0	37.0	37.0	33.0	37.0
33	35.52118020271129	37.0	37.0	37.0	33.0	37.0
34	35.50082544076016	37.0	37.0	37.0	33.0	37.0
35	35.44950261609157	37.0	37.0	37.0	33.0	37.0
36	35.46824201166959	37.0	37.0	37.0	33.0	37.0
37	35.467804373403965	37.0	37.0	37.0	33.0	37.0
38	35.452637114245356	37.0	37.0	37.0	33.0	37.0
39	35.349273142414596	37.0	37.0	37.0	33.0	37.0
40	35.13593479877232	37.0	37.0	37.0	33.0	37.0
41	35.30709145837797	37.0	37.0	37.0	33.0	37.0
42	35.353201575956014	37.0	37.0	37.0	33.0	37.0
43	35.022827074456934	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	22.0
16	40.0
17	45.0
18	35.0
19	41.0
20	46.0
21	124.0
22	367.0
23	821.0
24	1705.0
25	3236.0
26	5156.0
27	7970.0
28	11429.0
29	15379.0
30	20251.0
31	26458.0
32	34236.0
33	45365.0
34	65677.0
35	133890.0
36	500572.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.1581604070265	20.86652376593456	14.169741781966783	23.80557404507216
2	15.610969368758356	22.235919103368555	39.5020088971172	22.651102630755886
3	17.63498906477161	27.567430089578366	30.97642596180174	23.821154883848283
4	12.280450515370612	18.226029853345356	40.21483227112492	29.278687360159104
5	13.3626314203653	37.48967483018604	35.63807544562918	13.509618303819481
6	28.260891980106933	40.629557538548255	17.81829305037308	13.29125743097173
7	25.604358968777603	33.11902042350094	23.63246634366977	17.644154264051682
8	24.23530732631661	35.63372197597114	21.120170656010593	19.010800041701657
9	25.90555033011902	15.542803199112809	20.537034851815914	38.01461161895226
10	14.670849052604806	28.234771162158722	34.31691196940657	22.7774678158299
11	32.90799171007725	24.21319628305343	24.679246666445174	18.199565340424144
12	22.315770902096197	26.150146585905986	30.927735840626347	20.60634667137147
13	27.708001333536497	22.744358533430635	26.98372146042868	22.56391867260419
14	21.911012788889945	21.698036470619236	28.581903084891515	27.809047655599308
15	23.55043781011311	28.77826747946709	25.40971304906704	22.26158166135276
16	22.205788510735314	29.13089852176792	25.729807633923613	22.933505333573155
17	21.79106324331198	27.991435121272772	27.09118342198754	23.126318213427705
18	22.572511046929257	27.322146443845398	28.546387937681228	21.558954571544117
19	24.316763034918264	26.62994476821784	28.47455568832365	20.578736508540246
20	24.05223247069714	26.242142273679725	28.474097428359645	21.231527827263488
21	22.5533786934321	27.28857890148213	27.980551447127684	22.177490957958085
22	21.942174466442196	27.37977263431886	28.160647612981133	22.517405286257816
23	22.1046276236815	27.165077841183134	28.76096816582595	21.969326369309414
24	22.536079379790966	27.212393182466517	27.852467787188655	22.399059650553866
25	22.658091095206945	27.00778010853887	28.470775043620623	21.863353752633564
26	22.653508495566907	27.31137733469131	28.10634380724669	21.928770362495086
27	22.65637262034193	27.154423297020053	27.749932120242832	22.43927196239519
28	22.06017640717314	27.346777916910597	28.447862045420436	22.145183630495826
29	22.963177666242395	27.313439504529324	27.757951669612897	21.96543115961538
30	22.49185729326461	27.26119786863291	28.392641719757993	21.85430311834449
31	22.655914360377928	26.965276496877532	28.08365993902851	22.29514920371603
32	21.588626904213356	26.96000650729149	28.546387937681228	22.904978650813927
33	21.875841336652663	26.965963886823534	28.871752512123837	22.286442264399962
34	22.10073241398747	26.979482555761646	28.318289040598394	22.60149598965249
35	22.291368559013	27.61646390572676	28.110353581931726	21.981813953328512
36	22.5533786934321	27.14984069738001	28.187341255884345	22.10943935330354
37	22.811608183148177	26.625362168577805	27.948587814638426	22.614441833635592
38	22.69234602751622	27.058303269570278	27.987081651614737	22.262269051298766
39	22.2416473529186	26.9613812871835	28.267307619602988	22.529663740294914
40	22.315656337105196	27.40692453718608	28.283346718343118	21.99407240736561
41	22.22114021952944	26.767651887400945	28.4681400488276	22.54306784424202
42	22.067623131588203	27.28296521692308	28.21128533900354	22.438126312485178
43	22.34028781017039	26.775327741798005	28.202120139723462	22.682264308308138
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	73.0
2	98.0
3	289.0
4	480.0
5	480.0
6	622.0
7	764.0
8	699.5
9	635.0
10	934.5
11	1234.0
12	1234.0
13	1900.5
14	2567.0
15	4678.0
16	6789.0
17	7929.5
18	9070.0
19	9070.0
20	8943.5
21	8817.0
22	10123.0
23	11429.0
24	14047.0
25	16665.0
26	16665.0
27	19737.5
28	22810.0
29	27052.5
30	31295.0
31	35062.5
32	38830.0
33	38830.0
34	42856.5
35	46883.0
36	50059.5
37	53236.0
38	56358.5
39	59481.0
40	59481.0
41	61925.0
42	64369.0
43	63657.0
44	62945.0
45	63177.0
46	63409.0
47	63409.0
48	62412.0
49	61415.0
50	61767.0
51	62119.0
52	61933.5
53	61748.0
54	61748.0
55	56301.5
56	50855.0
57	45179.5
58	39504.0
59	35041.5
60	30579.0
61	30579.0
62	26823.5
63	23068.0
64	18679.5
65	14291.0
66	12235.0
67	10179.0
68	10179.0
69	8359.5
70	6540.0
71	5535.5
72	4531.0
73	3688.5
74	2846.0
75	2846.0
76	2134.0
77	1422.0
78	1107.5
79	793.0
80	618.5
81	444.0
82	444.0
83	364.0
84	284.0
85	246.5
86	209.0
87	159.0
88	109.0
89	109.0
90	82.5
91	56.0
92	36.5
93	17.0
94	10.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	872867.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.45546711798015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.70863223933499	48.387208691148295
2	8.404979148220626	9.490140478593567
3	2.1937445974811975	3.715466279650391
4	0.9356817553809097	2.1129740229520357
5	0.5554924795396279	1.568029370646736
6	0.34734541582398826	1.1765728620979385
7	0.2297441450115046	0.9079219116971824
8	0.17656087759264502	0.797426145540263
9	0.1316932926690223	0.6691325718541072
>10	1.046478181193685	12.047143099850812
>50	0.17187593261831774	6.756052006081124
>100	0.09406689338351916	10.01052101561257
>500	0.00288169913889891	1.0291157244278923
>1k	8.233426111139743E-4	1.3322958198470851
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4922	0.5638888857065281	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3521	0.4033833333142392	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1972	0.22592216225381417	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1050	0.12029324055096596	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	944	0.10814935150486844	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.291299820018399E-4	0.0	0.0
2	1.1456499100091995E-4	0.0	2.291299820018399E-4	0.0	0.0
3	1.1456499100091995E-4	0.0	2.291299820018399E-4	0.0	0.0
4	1.1456499100091995E-4	0.0	2.291299820018399E-4	1.1456499100091995E-4	0.0
5	1.1456499100091995E-4	0.0	3.4369497300275987E-4	2.291299820018399E-4	0.0
6	1.1456499100091995E-4	0.0	0.0011456499100091996	2.291299820018399E-4	0.0
7	1.1456499100091995E-4	0.0	0.0020621698380165593	3.4369497300275987E-4	0.0
8	1.1456499100091995E-4	0.0	0.002405864811019319	3.4369497300275987E-4	0.0
9	1.1456499100091995E-4	0.0	0.002978689766023919	9.165199280073596E-4	0.0
10	3.4369497300275987E-4	0.0	0.0033223847390266787	0.0010310849190082797	0.0
11	3.4369497300275987E-4	0.0	0.005957379532047838	0.0012602149010101195	0.0
12	3.4369497300275987E-4	0.0	0.006644769478053357	0.0012602149010101195	0.0
13	3.4369497300275987E-4	0.0	0.007561289406060717	0.0013747798920110395	0.0
14	3.4369497300275987E-4	0.0	0.008134114361065316	0.0014893448830119594	0.0
15	3.4369497300275987E-4	0.0	0.008477809334068076	0.0014893448830119594	0.0
16	3.4369497300275987E-4	0.0	0.008936069298071756	0.0016039098740128794	0.0
17	3.4369497300275987E-4	0.0	0.009165199280073597	0.0017184748650137993	0.0
18	4.582599640036798E-4	0.0	0.009508894253076357	0.0018330398560147192	0.0
19	4.582599640036798E-4	0.0	0.009508894253076357	0.0019476048470156392	0.0
20	4.582599640036798E-4	0.0	0.009852589226079116	0.0020621698380165593	0.0
21	4.582599640036798E-4	0.0	0.010196284199081876	0.0021767348290174793	0.0
22	4.582599640036798E-4	0.0	0.010310849190082795	0.002405864811019319	0.0
23	4.582599640036798E-4	0.0	0.010425414181083717	0.002749559784022079	0.0
24	4.582599640036798E-4	0.0	0.010425414181083717	0.0034369497300275986	0.0
25	4.582599640036798E-4	0.0	0.010425414181083717	0.0035515147210285185	0.0
26	4.582599640036798E-4	0.0	0.010425414181083717	0.0036660797120294385	0.0
27	4.582599640036798E-4	0.0	0.010425414181083717	0.004468034649035878	0.0
28	4.582599640036798E-4	0.0	0.010425414181083717	0.013289538956106715	0.0
29	4.582599640036798E-4	0.0	0.010425414181083717	0.03150537252525299	0.0
30	4.582599640036798E-4	0.0	0.010425414181083717	0.05384554577043238	0.0
31	4.582599640036798E-4	0.0	0.010425414181083717	0.11467955599192088	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2225	0.0	25.110111	1
GGTATCA	740	0.0	24.0	1
TCGATTC	45	0.0038250822	20.555555	37
TCGGCGT	55	5.141732E-4	20.181818	11
GTCAACC	225	0.0	18.911112	15
ATTACCC	255	0.0	18.862747	3
GTATTAG	305	0.0	18.80328	1
CGGCGTA	60	9.234388E-4	18.5	12
GCGAACT	140	9.458745E-11	18.5	28
CAACCCA	250	0.0	18.5	17
CATCGCT	70	1.2187706E-4	18.5	9
ATCGGCG	60	9.234388E-4	18.5	10
CTCGTCT	170	0.0	18.499998	13
CACGATA	50	0.00703356	18.499998	21
TGACTCG	50	0.00703356	18.499998	10
TCAACCC	245	0.0	18.122448	16
TACCCTC	285	0.0	17.526316	5
ATCAACG	3200	0.0	17.228125	3
TTAACGG	205	0.0	17.146341	35
GCGCATA	65	0.0015795222	17.076923	11
>>END_MODULE