FastQCFastQC Report
Fri 10 Feb 2017
ERR1633459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820711
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37320.45472766905768297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25510.31082805031247296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15430.18800771526152324No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC13800.16814688727213356No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12230.1490171327056662No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11930.1453617655910546No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10050.12245479833948855No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9500.11575329196270062No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9380.114291145116856No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9170.11173238813662788No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8270.10076628679279308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.025.5539571
TACCGGC400.001930719523.12530
GTATCAA18400.021.9184781
TGCACCG806.955124E-720.81255
CGGATCG555.1413703E-420.18181837
AACGGCC2600.018.537
TTAACGG2500.018.535
TCAAGGG1601.8189894E-1218.53
TAACGGC2600.017.78846236
CGAGAGA752.0663594E-417.26666610
ATTAGAG3350.017.1194043
GTATTAG4650.017.1075271
CCTCTAG1851.8189894E-1217.01
TATTAGA3750.016.7733352
GTGACAC2950.016.30508424
TTTAACG2850.016.22807134
GTATTAT1059.336509E-615.8571421
CTCTAGC2003.6379788E-1215.7252
GCACCGC2950.015.67796610
CTTGCGG957.055744E-515.57894834
Produced by FastQC (version 0.11.5)