FastQCFastQC Report
Fri 10 Feb 2017
ERR1633458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978853
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47240.48260566193289495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33480.342032971242873No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC19150.19563713856932552No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG18530.1893031946574205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17700.18082388264632177No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC16790.17152728754981594No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA15730.16069828666817182No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC15020.15344489928518376No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC14550.14864336115841703No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG12930.13209337867892318No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG12640.12913072749432244No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG12250.1251464724529628No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12110.12371622705350037No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG11710.11962981162646484No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC11690.11942549085511306No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT11200.11441963195699457No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA11130.11370450925726336No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10980.11217210347212501No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10860.11094617884401436No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA10510.1073705653453583No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG10080.1029776687612951No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT9910.101240942204805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8950.023.5642471
ATCTCGT400.001930983523.12537
GTATCAA23850.022.6498951
GCCGGCA855.1788447E-821.76470615
ATAACGC705.1006464E-621.1428593
TTAACGG4050.020.55555535
AACACCG656.900793E-519.9230775
AACGGCC4100.019.40244137
TAACGGC4400.018.92045436
GTCTTAC500.00703415618.4999981
AATACGC500.00703415618.4999985
TTTAACG4900.017.36734634
GGATCTA752.066872E-417.26666620
AACGCTC752.066872E-417.2666665
TCGGCGT650.001579707617.07692311
CACATGT5000.017.0228
GTACATG5200.016.7211531
GTATTAT1554.0017767E-1016.7096791
GCGGTAA904.4448727E-516.44444523
CCGCTCT904.4448727E-516.44444528
Produced by FastQC (version 0.11.5)