##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633458.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	978853
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.895288669493784	33.0	33.0	33.0	33.0	33.0
2	32.00272257427826	33.0	33.0	33.0	33.0	33.0
3	31.721561868840368	33.0	33.0	33.0	27.0	33.0
4	32.00748631306233	33.0	33.0	33.0	33.0	33.0
5	32.1054417772638	33.0	33.0	33.0	33.0	33.0
6	35.272433143689604	37.0	37.0	37.0	33.0	37.0
7	35.423719394025454	37.0	37.0	37.0	33.0	37.0
8	35.54709031897537	37.0	37.0	37.0	33.0	37.0
9	35.670724817720334	37.0	37.0	37.0	33.0	37.0
10	35.668737798218935	37.0	37.0	37.0	33.0	37.0
11	35.69054801895688	37.0	37.0	37.0	33.0	37.0
12	35.6740899808245	37.0	37.0	37.0	33.0	37.0
13	35.678692306199196	37.0	37.0	37.0	33.0	37.0
14	35.65666959185904	37.0	37.0	37.0	33.0	37.0
15	35.65872301561113	37.0	37.0	37.0	33.0	37.0
16	35.65798950404198	37.0	37.0	37.0	33.0	37.0
17	35.64865510960277	37.0	37.0	37.0	33.0	37.0
18	35.63779035258614	37.0	37.0	37.0	33.0	37.0
19	35.625624072255995	37.0	37.0	37.0	33.0	37.0
20	35.62604190823341	37.0	37.0	37.0	33.0	37.0
21	35.62222723943228	37.0	37.0	37.0	33.0	37.0
22	35.528570684260046	37.0	37.0	37.0	33.0	37.0
23	35.573588679811984	37.0	37.0	37.0	33.0	37.0
24	35.56742636534801	37.0	37.0	37.0	33.0	37.0
25	35.5875345940606	37.0	37.0	37.0	33.0	37.0
26	35.48183945904032	37.0	37.0	37.0	33.0	37.0
27	35.48955563297042	37.0	37.0	37.0	33.0	37.0
28	35.51752510336077	37.0	37.0	37.0	33.0	37.0
29	35.51543898828527	37.0	37.0	37.0	33.0	37.0
30	35.521836271636296	37.0	37.0	37.0	33.0	37.0
31	35.5101532099304	37.0	37.0	37.0	33.0	37.0
32	35.50525461943724	37.0	37.0	37.0	33.0	37.0
33	35.50957396054362	37.0	37.0	37.0	33.0	37.0
34	35.490400499359964	37.0	37.0	37.0	33.0	37.0
35	35.43061828487015	37.0	37.0	37.0	33.0	37.0
36	35.454693401358526	37.0	37.0	37.0	33.0	37.0
37	35.45080006906042	37.0	37.0	37.0	33.0	37.0
38	35.443259610993685	37.0	37.0	37.0	33.0	37.0
39	35.347142012130526	37.0	37.0	37.0	33.0	37.0
40	35.138850266587525	37.0	37.0	37.0	33.0	37.0
41	35.31781993823383	37.0	37.0	37.0	33.0	37.0
42	35.355840968970824	37.0	37.0	37.0	33.0	37.0
43	35.02681097161678	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	30.0
16	39.0
17	41.0
18	29.0
19	31.0
20	62.0
21	156.0
22	426.0
23	985.0
24	2073.0
25	3621.0
26	5950.0
27	9070.0
28	12967.0
29	17742.0
30	22980.0
31	29823.0
32	38703.0
33	50747.0
34	74375.0
35	151961.0
36	557035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.344001601874844	20.694016364050576	13.459630812798245	24.50235122127633
2	16.356490709023724	21.233934002347645	39.033235838271935	23.376339450356692
3	17.619908198677432	27.48247183182766	29.738070987165592	25.159548982329316
4	12.274570338957943	17.874185398624718	40.06485141282705	29.78639284959029
5	13.547897386022212	37.50123869467632	35.44669117834854	13.504172740952933
6	29.18334009294552	40.33179650059815	17.21300338253037	13.271860023925964
7	26.637094640359688	32.32538491479313	23.48023656258907	17.557283882258112
8	24.433903762873484	35.60340520997535	20.461090684709553	19.501600342441613
9	26.123023579638616	15.211170625211345	20.333083721457665	38.33272207369237
10	15.564134757721536	27.638368580369065	33.62568230367583	23.171814358233565
11	33.766561475522884	23.891227794163168	23.990834170197157	18.35137656011679
12	23.46481034435201	26.008297466524592	29.478277126391806	21.048615062731585
13	27.395226862460454	22.612792727815105	25.976219105422366	24.015761304302078
14	22.114147885331096	20.96096145182167	27.842485030949486	29.08240563189774
15	24.196585186948397	28.334285127593212	25.177427049822597	22.29170263563579
16	22.92969424418171	28.24550775244087	25.007840809600623	23.816957193776798
17	22.2840406067101	27.51843228758557	26.174205932862236	24.023321172842092
18	22.729255567485616	27.144729596783172	28.117602949574653	22.008411886156555
19	25.079863881502124	25.838609065916945	27.40564722179939	21.675879830781536
20	24.14908060760911	26.050898347351442	27.885801034476067	21.914220010563383
21	23.349982070852313	26.887489745651287	27.588718632930583	22.173809550565814
22	22.755102145061617	26.88299468868155	27.447022177998125	22.914880988258705
23	22.738041360653742	26.84897528025148	28.154482848803653	22.258500510291128
24	23.254768591402385	27.326983724828956	26.053860998536045	23.364386685232613
25	23.118793118067778	26.95849121369603	27.730517248248717	22.192198419987474
26	23.262328459942403	27.130018501245846	27.6996648117746	21.907988227037155
27	23.375420006885612	26.893925849948868	26.660795849836493	23.06985829332903
28	22.27433537007089	26.727506581682846	28.30741694616046	22.69074110208581
29	24.32816776369894	26.450141134572814	26.914868729012426	22.30682237271582
30	23.546538652892725	26.099935332475866	27.9619105218046	22.39161549282681
31	23.05198022583575	26.474353145977997	27.653999119377477	22.81966750880878
32	21.831470098165916	26.27330150696785	28.156526056517166	23.738702338349068
33	22.20169933585533	26.59306351413338	28.335306731449972	22.869930418561317
34	22.989355909416428	26.560167869945744	28.02943853673636	22.421037683901464
35	23.143924572944048	27.167000560860515	27.25056775634339	22.43850710985204
36	24.01514834198802	26.820881174190607	26.728221704382577	22.43574877943879
37	23.01622409084919	26.246331165149417	27.20173509199032	23.53570965201108
38	23.429565011293832	26.609307015455848	27.10825833909688	22.852869634153443
39	22.3501383762424	26.80617007865328	27.698949689074865	23.144741856029455
40	22.794638214318187	27.444468168356227	27.66544108257317	22.09545253475241
41	22.56528814847582	25.939543526964727	27.976212975799225	23.518955348760233
42	22.06715410792019	27.386032427749623	27.608537747751704	22.938275716578485
43	23.301966689584646	25.77547394756925	27.57022760312325	23.35233175972286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	60.0
2	71.0
3	286.0
4	501.0
5	501.0
6	582.5
7	664.0
8	617.0
9	570.0
10	803.0
11	1036.0
12	1036.0
13	1626.5
14	2217.0
15	4182.0
16	6147.0
17	6988.5
18	7830.0
19	7830.0
20	7169.5
21	6509.0
22	6955.5
23	7402.0
24	9614.5
25	11827.0
26	11827.0
27	15413.5
28	19000.0
29	22370.0
30	25740.0
31	31005.5
32	36271.0
33	36271.0
34	44702.0
35	53133.0
36	57629.5
37	62126.0
38	67028.0
39	71930.0
40	71930.0
41	75048.0
42	78166.0
43	80186.0
44	82206.0
45	79159.0
46	76112.0
47	76112.0
48	72904.0
49	69696.0
50	69766.0
51	69836.0
52	67221.0
53	64606.0
54	64606.0
55	63841.5
56	63077.0
57	53888.5
58	44700.0
59	40087.0
60	35474.0
61	35474.0
62	31984.0
63	28494.0
64	22725.0
65	16956.0
66	14018.5
67	11081.0
68	11081.0
69	9245.0
70	7409.0
71	6455.5
72	5502.0
73	5457.0
74	5412.0
75	5412.0
76	4645.5
77	3879.0
78	2873.0
79	1867.0
80	1226.0
81	585.0
82	585.0
83	464.5
84	344.0
85	281.5
86	219.0
87	176.0
88	133.0
89	133.0
90	95.0
91	57.0
92	36.5
93	16.0
94	9.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	978853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.655359556455615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.47327489062934	40.73259647380711
2	8.17032300322463	7.787193608220999
3	2.128016982492324	3.0423424332874625
4	0.9665527970125164	1.8424568428771728
5	0.5872301042957631	1.3992330881294757
6	0.3657483005327401	1.0457920061430188
7	0.27238387364325745	0.9086386005094723
8	0.20566282015002058	0.7840748513315116
9	0.16245629093226358	0.6967721660926662
>10	1.298170253519275	12.647467261599695
>50	0.19348948085867426	6.503206145016755
>100	0.15945835701663189	14.93238397259676
>500	0.01265196266040686	4.182073787566757
>1k	0.004580883032216277	3.4957687628211507
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4724	0.48260566193289495	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3348	0.342032971242873	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1915	0.19563713856932552	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1853	0.1893031946574205	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1770	0.18082388264632177	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1679	0.17152728754981594	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1573	0.16069828666817182	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1502	0.15344489928518376	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1455	0.14864336115841703	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1293	0.13209337867892318	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1264	0.12913072749432244	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1225	0.1251464724529628	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1211	0.12371622705350037	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1171	0.11962981162646484	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1169	0.11942549085511306	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	1120	0.11441963195699457	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1113	0.11370450925726336	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1098	0.11217210347212501	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1086	0.11094617884401436	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1051	0.1073705653453583	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	1008	0.1029776687612951	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	991	0.101240942204805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.02160385675888E-4	0.0	0.0
2	0.0	0.0	1.02160385675888E-4	2.04320771351776E-4	0.0
3	0.0	0.0	1.02160385675888E-4	2.04320771351776E-4	0.0
4	0.0	0.0	2.04320771351776E-4	3.0648115702766403E-4	0.0
5	0.0	0.0	3.0648115702766403E-4	3.0648115702766403E-4	0.0
6	0.0	0.0	8.17283085407104E-4	3.0648115702766403E-4	0.0
7	0.0	0.0	0.00153240578513832	5.1080192837944E-4	0.0
8	1.02160385675888E-4	0.0	0.00204320771351776	6.129623140553281E-4	0.0
9	1.02160385675888E-4	0.0	0.002247528484869536	9.19443471082992E-4	0.0
10	1.02160385675888E-4	0.0	0.0024518492562213123	9.19443471082992E-4	0.0
11	1.02160385675888E-4	0.0	0.003269132341628416	0.001123764242434768	0.0
12	1.02160385675888E-4	0.0	0.003677773884331968	0.001123764242434768	0.0
13	1.02160385675888E-4	0.0	0.00408641542703552	0.001123764242434768	0.0
14	1.02160385675888E-4	0.0	0.004495056969739072	0.001123764242434768	0.0
15	1.02160385675888E-4	0.0	0.005005858898118512	0.001123764242434768	0.0
16	1.02160385675888E-4	0.0	0.005516660826497952	0.001123764242434768	0.0
17	1.02160385675888E-4	0.0	0.0056188212121738405	0.001123764242434768	0.0
18	2.04320771351776E-4	0.0	0.005925302369201504	0.001430245399462432	0.0
19	2.04320771351776E-4	0.0	0.005925302369201504	0.00153240578513832	0.0
20	2.04320771351776E-4	0.0	0.006231783526229168	0.00153240578513832	0.0
21	2.04320771351776E-4	0.0	0.0063339439119050565	0.002145368099193648	1.02160385675888E-4
22	2.04320771351776E-4	0.0	0.0063339439119050565	0.002349688870545424	1.02160385675888E-4
23	3.0648115702766403E-4	0.0	0.0063339439119050565	0.002758330413248976	1.02160385675888E-4
24	3.0648115702766403E-4	0.0	0.0063339439119050565	0.00306481157027664	1.02160385675888E-4
25	3.0648115702766403E-4	0.0	0.0063339439119050565	0.00357561349865608	1.02160385675888E-4
26	3.0648115702766403E-4	0.0	0.0063339439119050565	0.00408641542703552	1.02160385675888E-4
27	3.0648115702766403E-4	0.0	0.0063339439119050565	0.005312340055146176	1.02160385675888E-4
28	3.0648115702766403E-4	0.0	0.0063339439119050565	0.014506774765976097	1.02160385675888E-4
29	3.0648115702766403E-4	0.0	0.0063339439119050565	0.031465398788173504	1.02160385675888E-4
30	3.0648115702766403E-4	0.0	0.0063339439119050565	0.057107655592821396	1.02160385675888E-4
31	3.0648115702766403E-4	0.0	0.0063339439119050565	0.11768876429862297	1.02160385675888E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	895	0.0	23.564247	1
ATCTCGT	40	0.0019309835	23.125	37
GTATCAA	2385	0.0	22.649895	1
GCCGGCA	85	5.1788447E-8	21.764706	15
ATAACGC	70	5.1006464E-6	21.142859	3
TTAACGG	405	0.0	20.555555	35
AACACCG	65	6.900793E-5	19.923077	5
AACGGCC	410	0.0	19.402441	37
TAACGGC	440	0.0	18.920454	36
GTCTTAC	50	0.007034156	18.499998	1
AATACGC	50	0.007034156	18.499998	5
TTTAACG	490	0.0	17.367346	34
GGATCTA	75	2.066872E-4	17.266666	20
AACGCTC	75	2.066872E-4	17.266666	5
TCGGCGT	65	0.0015797076	17.076923	11
CACATGT	500	0.0	17.02	28
GTACATG	520	0.0	16.721153	1
GTATTAT	155	4.0017767E-10	16.709679	1
GCGGTAA	90	4.4448727E-5	16.444445	23
CCGCTCT	90	4.4448727E-5	16.444445	28
>>END_MODULE