FastQCFastQC Report
Fri 10 Feb 2017
ERR1633457.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633457.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807813
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39480.48872697022702033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27780.34389146993177877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15510.19199988116061514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCA358.8671024E-426.42857217
GGTATCA7850.025.216561
GTATCAA19500.021.156411
TTCGCCG1101.7516868E-920.18181824
GCGGTAA656.898886E-519.92307723
TCTGACG500.007033114318.58
AAATCGT500.007033114318.532
TACGTGA609.233577E-418.536
GTATTAG3350.017.6716421
GTATTAT1502.5102054E-1017.2666661
CCGTACA1950.017.07692317
AGCTTCG1201.0400436E-716.95833221
CCTACCG1005.8734367E-616.65000224
GCCGCTC1352.2162567E-816.44444527
GCACCGC2300.016.08695610
GCCGGCA1403.4680852E-815.85714315
CTAGGAC1403.4680852E-815.8571433
GGCTAGT700.002591598815.8571431
CCGCTCT1302.5859845E-715.65384628
GCGATAG1557.195922E-915.51612936
Produced by FastQC (version 0.11.5)