##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633457.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	807813
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.783482068250944	33.0	33.0	33.0	27.0	33.0
2	31.923953934883446	33.0	33.0	33.0	33.0	33.0
3	31.63970621913735	33.0	33.0	33.0	27.0	33.0
4	31.933052575286606	33.0	33.0	33.0	33.0	33.0
5	32.02745684954315	33.0	33.0	33.0	33.0	33.0
6	35.16899455690859	37.0	37.0	37.0	33.0	37.0
7	35.3288607635678	37.0	37.0	37.0	33.0	37.0
8	35.43859903220176	37.0	37.0	37.0	33.0	37.0
9	35.56145172211885	37.0	37.0	37.0	33.0	37.0
10	35.570660536535065	37.0	37.0	37.0	33.0	37.0
11	35.58471329379448	37.0	37.0	37.0	33.0	37.0
12	35.587097508953185	37.0	37.0	37.0	33.0	37.0
13	35.57498950871056	37.0	37.0	37.0	33.0	37.0
14	35.55565458837627	37.0	37.0	37.0	33.0	37.0
15	35.57134014926722	37.0	37.0	37.0	33.0	37.0
16	35.55303269444785	37.0	37.0	37.0	33.0	37.0
17	35.547683684218995	37.0	37.0	37.0	33.0	37.0
18	35.52739185925456	37.0	37.0	37.0	33.0	37.0
19	35.51169268135076	37.0	37.0	37.0	33.0	37.0
20	35.52284872860427	37.0	37.0	37.0	33.0	37.0
21	35.51449283435647	37.0	37.0	37.0	33.0	37.0
22	35.41785784581332	37.0	37.0	37.0	33.0	37.0
23	35.46489472192203	37.0	37.0	37.0	33.0	37.0
24	35.46152141646643	37.0	37.0	37.0	33.0	37.0
25	35.476841793831	37.0	37.0	37.0	33.0	37.0
26	35.366099579977046	37.0	37.0	37.0	33.0	37.0
27	35.387781578162276	37.0	37.0	37.0	33.0	37.0
28	35.3987804108129	37.0	37.0	37.0	33.0	37.0
29	35.40932988203953	37.0	37.0	37.0	33.0	37.0
30	35.40233197534578	37.0	37.0	37.0	33.0	37.0
31	35.395696776357894	37.0	37.0	37.0	33.0	37.0
32	35.387754344136575	37.0	37.0	37.0	33.0	37.0
33	35.37982181519733	37.0	37.0	37.0	33.0	37.0
34	35.36401865283178	37.0	37.0	37.0	33.0	37.0
35	35.30351331310588	37.0	37.0	37.0	33.0	37.0
36	35.32674888866607	37.0	37.0	37.0	33.0	37.0
37	35.31697434926152	37.0	37.0	37.0	33.0	37.0
38	35.307442440267735	37.0	37.0	37.0	33.0	37.0
39	35.211368225071894	37.0	37.0	37.0	33.0	37.0
40	35.00435620620119	37.0	37.0	37.0	27.0	37.0
41	35.168354557304724	37.0	37.0	37.0	33.0	37.0
42	35.219232668946894	37.0	37.0	37.0	33.0	37.0
43	34.87533748528434	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	11.0
15	25.0
16	33.0
17	26.0
18	23.0
19	34.0
20	58.0
21	183.0
22	438.0
23	926.0
24	1976.0
25	3629.0
26	5654.0
27	8454.0
28	11829.0
29	15996.0
30	20659.0
31	26476.0
32	33325.0
33	44148.0
34	63639.0
35	128933.0
36	441338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.93620677062637	20.96215336965362	13.870784451351984	24.230855408368026
2	15.80489543990998	21.885015467688685	39.06374371296327	23.246345379438065
3	17.769830393915424	27.53954194844599	30.17418635253456	24.516441305104028
4	12.342212863620665	18.22488620509945	39.97088435070988	29.462016580570012
5	13.653159827831441	37.31049141323549	35.517502194195934	13.518846564737135
6	28.9021097704543	40.544779546751535	17.46926578304632	13.083844899747838
7	26.00539976454947	32.663128719146634	23.771466911277734	17.560004605026165
8	24.3639307612034	36.09015947997866	20.687832456273913	18.85807730254403
9	25.81414262954421	15.49331342773637	20.641410821563902	38.051133121155516
10	14.763317748043173	28.306798726933092	34.11631157210889	22.813571952914845
11	33.51258273882693	23.964457120645495	24.504557366618265	18.018402773909308
12	22.736821516860957	26.04365119155052	30.74585331011014	20.473673981478388
13	27.83317426186506	22.765541034868217	26.37404943965992	23.027235263606798
14	21.798485540589223	21.278810813888857	28.199471907483538	28.723231738038386
15	23.49244193891408	29.11663961832751	24.924827899526253	22.46609054323216
16	22.511645640760918	28.729916453436626	25.740734551189448	23.017703354613012
17	22.115142984824455	27.966002032648646	26.74331806989984	23.175536912627056
18	22.742268322000264	27.478389181654666	28.143270781727946	21.63607171461712
19	24.205849621137567	26.79407239051612	27.93233087360565	21.067747114740666
20	23.873965880717442	26.49635497324257	28.0321064404757	21.597572705564282
21	22.77767255540577	27.230311965764354	28.021336621222982	21.970678857606895
22	22.378941661003225	27.20122107467941	27.9631548390531	22.456682425264262
23	22.41087974568372	26.908950462545167	28.49199010166957	22.188179690101546
24	22.591986016565716	27.48321703166451	27.25544154402071	22.66935540774907
25	22.707235461672443	27.250985067088546	28.064292107207976	21.97748736403103
26	22.758113573314617	27.328725831349583	27.95783182493968	21.955328770396118
27	22.656233559004374	27.329220995453156	27.56566185490949	22.44888359063298
28	22.306400119829714	27.068888467999404	28.27931711918476	22.345394292986125
29	23.382391716894876	27.009097402492905	27.529391084322736	22.079119796289486
30	22.913966474914368	26.856091694488697	28.106257264985835	22.1236845656111
31	22.934887158290344	26.88988664455759	27.939882126185147	22.23534407096692
32	21.88637716897351	26.93779377157832	28.234876140889043	22.94095291855912
33	21.9818200499373	26.979263765252604	28.55809450949663	22.48082167531347
34	22.50409438818142	26.82972420597341	28.27040416532044	22.395777240524726
35	22.461138902196424	27.541646395886175	27.747387080920955	22.24982762099644
36	23.178384106222605	27.115805266812988	27.52122087661377	22.18458975035064
37	22.64992021668381	26.729453474999783	27.74305439501469	22.87757191330172
38	22.724566205297513	27.11691938604603	27.678311688472455	22.480202720184003
39	22.489487047126005	26.853739664996723	28.004996205805057	22.651777082072215
40	22.39639619565419	27.575317554929175	28.10774275729655	21.920543492120082
41	22.482554749675977	26.516037746359615	28.107495175244768	22.893912328719644
42	21.985409989688208	27.53075278560756	28.0359439622784	22.447893262425833
43	22.78212903233793	26.650474800479813	27.89123225300905	22.676163914173205
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	62.0
2	77.0
3	252.5
4	428.0
5	428.0
6	523.5
7	619.0
8	562.0
9	505.0
10	707.0
11	909.0
12	909.0
13	1471.5
14	2034.0
15	3660.5
16	5287.0
17	6223.5
18	7160.0
19	7160.0
20	6944.0
21	6728.0
22	7572.5
23	8417.0
24	10767.0
25	13117.0
26	13117.0
27	16430.0
28	19743.0
29	23027.0
30	26311.0
31	30302.5
32	34294.0
33	34294.0
34	39713.5
35	45133.0
36	48040.0
37	50947.0
38	54068.5
39	57190.0
40	57190.0
41	59381.0
42	61572.0
43	62522.0
44	63472.0
45	62560.5
46	61649.0
47	61649.0
48	59948.5
49	58248.0
50	57573.0
51	56898.0
52	54443.5
53	51989.0
54	51989.0
55	49648.5
56	47308.0
57	41607.0
58	35906.0
59	32727.0
60	29548.0
61	29548.0
62	25840.0
63	22132.0
64	18195.5
65	14259.0
66	12068.0
67	9877.0
68	9877.0
69	8152.0
70	6427.0
71	5246.5
72	4066.0
73	3188.5
74	2311.0
75	2311.0
76	1816.5
77	1322.0
78	1031.0
79	740.0
80	576.5
81	413.0
82	413.0
83	358.5
84	304.0
85	261.5
86	219.0
87	169.5
88	120.0
89	120.0
90	91.5
91	63.0
92	41.5
93	20.0
94	12.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	807813.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.31479296635259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.95359045529612	53.56180193936439
2	8.325333717740104	10.375828939935385
3	2.224954037796755	4.159426506748652
4	0.9838651268356204	2.452374067423037
5	0.5665427781804846	1.7651997964449562
6	0.3531968047939737	1.3205631460268235
7	0.26118255247315514	1.1392875678651733
8	0.18232516232982324	0.9089243794511651
9	0.1429260895248201	0.8015768710406589
>10	0.8520117394904889	10.211129421638319
>50	0.08878784667657505	3.8087244716754016
>100	0.06206972264354606	7.382734489639082
>500	0.002611347552593442	1.0763671388493408
>1k	6.026186659831019E-4	1.0360612638976618
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3948	0.48872697022702033	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2778	0.34389146993177877	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1551	0.19199988116061514	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.2379102589336888E-4	0.0	0.0
3	0.0	0.0	3.7137307768010663E-4	0.0	0.0
4	0.0	0.0	4.951641035734755E-4	1.2379102589336888E-4	0.0
5	0.0	0.0	4.951641035734755E-4	1.2379102589336888E-4	0.0
6	0.0	0.0	8.665371812535823E-4	1.2379102589336888E-4	0.0
7	0.0	0.0	0.00222823846608064	2.4758205178673776E-4	0.0
8	1.2379102589336888E-4	0.0	0.0025996115437607464	2.4758205178673776E-4	0.0
9	1.2379102589336888E-4	0.0	0.002847193595547484	6.189551294668444E-4	0.0
10	1.2379102589336888E-4	0.0	0.00334235769912096	6.189551294668444E-4	0.0
11	1.2379102589336888E-4	0.0	0.0038375218026944356	7.427461553602133E-4	0.0
12	1.2379102589336888E-4	0.0	0.004085103854481173	7.427461553602133E-4	0.0
13	1.2379102589336888E-4	0.0	0.0045802679580546485	7.427461553602133E-4	0.0
14	1.2379102589336888E-4	0.0	0.004951641035734755	8.665371812535823E-4	0.0
15	1.2379102589336888E-4	0.0	0.005323014113414862	0.00111411923304032	0.0
16	1.2379102589336888E-4	0.0	0.005941969242881706	0.0012379102589336888	0.0
17	1.2379102589336888E-4	0.0	0.006065760268775075	0.0013617012848270577	0.0
18	1.2379102589336888E-4	0.0	0.006313342320561813	0.0013617012848270577	0.0
19	1.2379102589336888E-4	0.0	0.006313342320561813	0.0014854923107204265	0.0
20	1.2379102589336888E-4	0.0	0.006437133346455182	0.0014854923107204265	0.0
21	1.2379102589336888E-4	0.0	0.006437133346455182	0.0017330743625071645	0.0
22	1.2379102589336888E-4	0.0	0.006560924372348551	0.0018568653884005334	0.0
23	1.2379102589336888E-4	0.0	0.00668471539824192	0.002104447440187271	0.0
24	1.2379102589336888E-4	0.0	0.006808506424135289	0.0025996115437607464	0.0
25	1.2379102589336888E-4	0.0	0.006808506424135289	0.002847193595547484	0.0
26	1.2379102589336888E-4	0.0	0.006808506424135289	0.00334235769912096	0.0
27	1.2379102589336888E-4	0.0	0.006808506424135289	0.004208894880374542	0.0
28	1.2379102589336888E-4	0.0	0.006808506424135289	0.01101740130450983	0.0
29	1.2379102589336888E-4	0.0	0.006808506424135289	0.024015459023313562	0.0
30	1.2379102589336888E-4	0.0	0.006808506424135289	0.04320306803678574	0.0
31	1.2379102589336888E-4	0.0	0.006808506424135289	0.09569046301557414	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGCA	35	8.8671024E-4	26.428572	17
GGTATCA	785	0.0	25.21656	1
GTATCAA	1950	0.0	21.15641	1
TTCGCCG	110	1.7516868E-9	20.181818	24
GCGGTAA	65	6.898886E-5	19.923077	23
TCTGACG	50	0.0070331143	18.5	8
AAATCGT	50	0.0070331143	18.5	32
TACGTGA	60	9.233577E-4	18.5	36
GTATTAG	335	0.0	17.671642	1
GTATTAT	150	2.5102054E-10	17.266666	1
CCGTACA	195	0.0	17.076923	17
AGCTTCG	120	1.0400436E-7	16.958332	21
CCTACCG	100	5.8734367E-6	16.650002	24
GCCGCTC	135	2.2162567E-8	16.444445	27
GCACCGC	230	0.0	16.086956	10
GCCGGCA	140	3.4680852E-8	15.857143	15
CTAGGAC	140	3.4680852E-8	15.857143	3
GGCTAGT	70	0.0025915988	15.857143	1
CCGCTCT	130	2.5859845E-7	15.653846	28
GCGATAG	155	7.195922E-9	15.516129	36
>>END_MODULE