FastQCFastQC Report
Fri 10 Feb 2017
ERR1633456.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633456.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774315
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33790.4363857086586208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24140.3117594260733681No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA20100.2595842777164332No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT19210.2480902475090887No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC17630.22768511523088147No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC17540.2265227975694646No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG15190.19617339196580202No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT14480.1870039970812912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14470.1868748506744671No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA14400.18597082582669844No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG13360.17253959951699244No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC13290.17163557466922377No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA13170.17008581778733461No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC12610.16285361900518525No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA12510.16156215493694429No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT11770.15200532083196114No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT11530.14890580706818285No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC10500.13560372716530095No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA10470.13521628794482865No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC10390.1341831166902359No Hit
ATCTAGCACTTTCATATTTTAAAGCTGATATTTTAGCAATATT8650.11171164190284316No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG8220.10615834640940704No Hit
GGAAATAACTATATCACTATTCAAGATCATCTTCACAACATCA8210.10602920000258294No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA8160.10538346796846246No Hit
GGTTGGGGTACTTCATCCAAAATAAGAAGAAAATAACAGAATC7910.10215480779786006No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG7900.10202566139103594No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC7880.10176736857738776No Hit
TTTTAAAGCTGATATTTTAGCAATATTTTGATAAAACTTTATT7760.10021761169549862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCC250.0054952529.59999810
GGTATCA6150.027.073171
GTATCAA18450.022.8617861
CTCCGTT450.003824696420.55555736
AAGACGT450.003824696420.5555575
ATAGCGG1001.2882083E-820.3499987
CGGGTAA555.1410106E-420.18181823
TTGGCGT555.1410106E-420.18181823
GCCGGCA656.898413E-519.92307715
GCCGCTC656.898413E-519.92307727
AGTTCGC1500.019.7333328
CGGCCTT2350.019.68085124
GTCGCCC1551.8189894E-1219.09677337
TCGGCCT2650.018.15094423
TCGCCAT2450.018.12244813
AGCGGCT1156.39684E-817.6956529
AATAGCG1156.39684E-817.6956526
GTATTAG2750.017.490911
AATCTCG2750.017.4909110
TAGTTCG1502.5102054E-1017.2666667
Produced by FastQC (version 0.11.5)