##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633456.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	774315
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.903150526594473	33.0	33.0	33.0	33.0	33.0
2	32.03276960926755	33.0	33.0	33.0	33.0	33.0
3	31.74932553289036	33.0	33.0	33.0	27.0	33.0
4	32.01566933353997	33.0	33.0	33.0	33.0	33.0
5	32.11292949251919	33.0	33.0	33.0	33.0	33.0
6	35.31346415864345	37.0	37.0	37.0	33.0	37.0
7	35.44802179991347	37.0	37.0	37.0	33.0	37.0
8	35.57095109871305	37.0	37.0	37.0	33.0	37.0
9	35.685323156596475	37.0	37.0	37.0	33.0	37.0
10	35.709206201610456	37.0	37.0	37.0	33.0	37.0
11	35.722753659686305	37.0	37.0	37.0	33.0	37.0
12	35.71529932908442	37.0	37.0	37.0	33.0	37.0
13	35.7169549860199	37.0	37.0	37.0	33.0	37.0
14	35.68578937512511	37.0	37.0	37.0	33.0	37.0
15	35.70696034559578	37.0	37.0	37.0	33.0	37.0
16	35.69109858390965	37.0	37.0	37.0	33.0	37.0
17	35.68443462931753	37.0	37.0	37.0	33.0	37.0
18	35.66660338492732	37.0	37.0	37.0	33.0	37.0
19	35.66083441493449	37.0	37.0	37.0	33.0	37.0
20	35.66660338492732	37.0	37.0	37.0	33.0	37.0
21	35.65825665265428	37.0	37.0	37.0	33.0	37.0
22	35.5802005643698	37.0	37.0	37.0	33.0	37.0
23	35.62662094883865	37.0	37.0	37.0	33.0	37.0
24	35.61296242485293	37.0	37.0	37.0	33.0	37.0
25	35.62932140020534	37.0	37.0	37.0	33.0	37.0
26	35.52403350057793	37.0	37.0	37.0	33.0	37.0
27	35.54246140136766	37.0	37.0	37.0	33.0	37.0
28	35.567859333733686	37.0	37.0	37.0	33.0	37.0
29	35.578600440389245	37.0	37.0	37.0	33.0	37.0
30	35.57282113868387	37.0	37.0	37.0	33.0	37.0
31	35.573417795083394	37.0	37.0	37.0	33.0	37.0
32	35.562656025002745	37.0	37.0	37.0	33.0	37.0
33	35.57010260682022	37.0	37.0	37.0	33.0	37.0
34	35.54524579789879	37.0	37.0	37.0	33.0	37.0
35	35.47876510205795	37.0	37.0	37.0	33.0	37.0
36	35.50808521079922	37.0	37.0	37.0	33.0	37.0
37	35.51769757785914	37.0	37.0	37.0	33.0	37.0
38	35.49990378592692	37.0	37.0	37.0	33.0	37.0
39	35.40512969527905	37.0	37.0	37.0	33.0	37.0
40	35.21171745349115	37.0	37.0	37.0	33.0	37.0
41	35.37487714948051	37.0	37.0	37.0	33.0	37.0
42	35.424452580668074	37.0	37.0	37.0	33.0	37.0
43	35.09914698798293	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	29.0
16	35.0
17	26.0
18	29.0
19	25.0
20	49.0
21	101.0
22	339.0
23	708.0
24	1449.0
25	2802.0
26	4585.0
27	6973.0
28	9862.0
29	13282.0
30	17517.0
31	22753.0
32	29866.0
33	39405.0
34	57370.0
35	118460.0
36	448647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.61628665336458	21.56577103633534	14.221473173062643	23.59646913723743
2	15.376042050070064	22.280983837327188	40.324932359569424	22.018041753033327
3	17.380135991166384	27.783524792881447	31.9734216694756	22.862917546476563
4	12.465340333068584	18.22049166037078	39.79827331254076	29.515894694019874
5	12.800475258777114	38.69407153419474	35.84652241013024	12.658930796897902
6	28.305534569264445	40.4903689067111	17.964781774859066	13.23931474916539
7	24.650949548956174	33.50910159302093	24.337252926780444	17.502695931242453
8	23.211096259274328	37.5565499828881	20.956845728159728	18.275508029677844
9	24.700800061990275	16.47481967932947	20.751244648495767	38.07313561018448
10	14.02620380594461	29.329278136159058	34.71274610462151	21.931771953274833
11	32.12607272234168	24.80850816528157	25.09120964981952	17.974209462557226
12	21.547948832193615	27.13185202404706	30.762544959092875	20.557654184666447
13	26.439239844249435	23.164345260004005	27.769447834537626	22.626967061208937
14	21.09270774813868	22.359117413455763	28.427319630899568	28.12085520750599
15	23.25888042979924	29.69889515248962	25.762125233270698	21.28009918444044
16	22.141893157177634	30.026410440195527	25.329097331189505	22.502599071437334
17	21.72810806971323	28.78169737122489	26.728915234755878	22.761279324306
18	20.973763907453684	28.49292600556621	29.01532322116968	21.517986865810425
19	23.639216597896205	27.576632249149245	29.09629801824839	19.68785313470616
20	23.390738911166643	26.763397325377912	29.447705391216754	20.39815837223869
21	21.320651156183207	28.726939294731473	27.980085624067723	21.972323925017594
22	20.9465140156138	28.806493481335117	28.090505801902328	22.156486701148754
23	21.166321200028413	28.38483046305444	28.68806622627742	21.760782110639727
24	22.84135009653694	28.803652260384986	27.216313774110017	21.138683868968055
25	22.368674247560747	27.561780412364477	28.461026843080656	21.608518496994115
26	21.932546831715776	27.838282869374865	28.268211257692283	21.960959041217077
27	21.740893563988816	27.69738413952978	28.060414689112314	22.501307607369096
28	20.766613070907837	28.254134299348454	28.982132594615884	21.997120035127825
29	22.661190859017324	28.441784028463868	27.692864015290937	21.20416109722787
30	21.492932462886554	28.625688511781384	28.335754828461283	21.545624196870783
31	22.13918108263433	27.968075008233086	28.564602261353585	21.328141647779006
32	20.885427765186005	28.129120577542732	28.602829597773514	22.382622059497752
33	21.26744283657168	27.58967603623848	29.210334295474066	21.932546831715776
34	21.30980285800998	28.06919664477635	28.43274377998618	22.188256717227482
35	21.993503935736747	28.685741590954585	27.59187152515449	21.728882948154173
36	22.303067872894104	28.52198394710163	27.49565745207054	21.679290727933722
37	22.58848143197536	27.18157339067434	27.63436069299962	22.595584484350685
38	22.503244803471457	28.263303694232967	27.963942323214713	21.269509179080863
39	21.82961714547697	27.867857396537588	28.468129895455984	21.834395562529462
40	22.231520763513558	27.763248807010065	28.28280480166341	21.722425627812967
41	21.335502992967978	28.193564634547958	28.65190523236667	21.819027140117395
42	21.93073878202024	27.88219264769506	28.215002938080758	21.97206563220395
43	21.72746233767911	27.56152211955083	28.174063527117514	22.536952015652545
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	62.0
2	68.0
3	250.0
4	432.0
5	432.0
6	484.0
7	536.0
8	490.0
9	444.0
10	689.5
11	935.0
12	935.0
13	1396.5
14	1858.0
15	3979.5
16	6101.0
17	6867.0
18	7633.0
19	7633.0
20	7633.5
21	7634.0
22	9363.5
23	11093.0
24	13288.5
25	15484.0
26	15484.0
27	18394.0
28	21304.0
29	27887.5
30	34471.0
31	35740.0
32	37009.0
33	37009.0
34	41201.5
35	45394.0
36	47170.5
37	48947.0
38	53942.0
39	58937.0
40	58937.0
41	62769.5
42	66602.0
43	61437.5
44	56273.0
45	56273.5
46	56274.0
47	56274.0
48	54762.0
49	53250.0
50	54681.0
51	56112.0
52	55588.5
53	55065.0
54	55065.0
55	46772.5
56	38480.0
57	33031.5
58	27583.0
59	23892.5
60	20202.0
61	20202.0
62	17911.5
63	15621.0
64	13027.0
65	10433.0
66	8663.0
67	6893.0
68	6893.0
69	5800.0
70	4707.0
71	4054.5
72	3402.0
73	2768.0
74	2134.0
75	2134.0
76	1652.0
77	1170.0
78	951.0
79	732.0
80	561.0
81	390.0
82	390.0
83	315.0
84	240.0
85	238.5
86	237.0
87	175.5
88	114.0
89	114.0
90	82.0
91	50.0
92	30.5
93	11.0
94	6.5
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	774315.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.57432789106239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.84341779632749	42.18360651219334
2	7.484929523611784	7.271508418428244
3	1.8852313322058531	2.747215346432144
4	0.9050562492650883	1.7584999604662996
5	0.520660551308843	1.2645368169608526
6	0.3499159209450426	1.019815840697254
7	0.23837609391599038	0.81052709830862
8	0.17319526567147464	0.6730274899124697
9	0.15062865887717822	0.6585017279481935
>10	1.088034203115281	10.879197420919452
>50	0.1741913488706238	5.9475595063384015
>100	0.16878163551882072	16.85518159654974
>500	0.01217175256145113	3.8506459078918747
>1k	0.005409667805089391	4.080176356953124
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3379	0.4363857086586208	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2414	0.3117594260733681	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2010	0.2595842777164332	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1921	0.2480902475090887	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1763	0.22768511523088147	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1754	0.2265227975694646	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1519	0.19617339196580202	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1448	0.1870039970812912	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1447	0.1868748506744671	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1440	0.18597082582669844	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1336	0.17253959951699244	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1329	0.17163557466922377	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1317	0.17008581778733461	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1261	0.16285361900518525	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1251	0.16156215493694429	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1177	0.15200532083196114	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1153	0.14890580706818285	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	1050	0.13560372716530095	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1047	0.13521628794482865	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1039	0.1341831166902359	No Hit
ATCTAGCACTTTCATATTTTAAAGCTGATATTTTAGCAATATT	865	0.11171164190284316	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	822	0.10615834640940704	No Hit
GGAAATAACTATATCACTATTCAAGATCATCTTCACAACATCA	821	0.10602920000258294	No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA	816	0.10538346796846246	No Hit
GGTTGGGGTACTTCATCCAAAATAAGAAGAAAATAACAGAATC	791	0.10215480779786006	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	790	0.10202566139103594	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	788	0.10176736857738776	No Hit
TTTTAAAGCTGATATTTTAGCAATATTTTGATAAAACTTTATT	776	0.10021761169549862	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2914640682409613E-4	0.0	2.5829281364819225E-4	0.0	0.0
2	1.2914640682409613E-4	0.0	2.5829281364819225E-4	1.2914640682409613E-4	0.0
3	1.2914640682409613E-4	0.0	3.8743922047228843E-4	2.5829281364819225E-4	0.0
4	1.2914640682409613E-4	0.0	5.165856272963845E-4	3.8743922047228843E-4	0.0
5	2.5829281364819225E-4	0.0	6.457320341204807E-4	3.8743922047228843E-4	0.0
6	2.5829281364819225E-4	0.0	9.04024847768673E-4	3.8743922047228843E-4	0.0
7	2.5829281364819225E-4	0.0	0.0032286601706024032	3.8743922047228843E-4	0.0
8	2.5829281364819225E-4	0.0	0.003874392204722884	3.8743922047228843E-4	0.0
9	2.5829281364819225E-4	0.0	0.00503670986613975	0.0011623176614168652	0.0
10	2.5829281364819225E-4	0.0	0.005295002679787941	0.0014206104750650575	0.0
11	3.8743922047228843E-4	0.0	0.0074904915957975755	0.0015497568818891537	0.0
12	3.8743922047228843E-4	0.0	0.008523662850390345	0.0015497568818891537	0.0
13	3.8743922047228843E-4	0.0	0.009427687698159017	0.0015497568818891537	0.0
14	3.8743922047228843E-4	0.0	0.009815126918631307	0.0015497568818891537	0.0
15	3.8743922047228843E-4	0.0	0.010331712545927692	0.001808049695537346	0.0
16	3.8743922047228843E-4	0.0	0.010848298173224075	0.001937196102361442	0.0
17	3.8743922047228843E-4	0.0	0.010977444580048172	0.001937196102361442	0.0
18	3.8743922047228843E-4	0.0	0.011494030207344557	0.0021954889160096342	0.0
19	3.8743922047228843E-4	0.0	0.011881469427816845	0.0023246353228337305	0.0
20	3.8743922047228843E-4	0.0	0.012139762241465037	0.0023246353228337305	0.0
21	3.8743922047228843E-4	0.0	0.01304378708923371	0.0027120745433060187	0.0
22	5.165856272963845E-4	0.0	0.013172933496057805	0.0030995137637783074	0.0
23	5.165856272963845E-4	0.0	0.013172933496057805	0.0033578065774264995	0.0
24	5.165856272963845E-4	0.0	0.013172933496057805	0.004132685018371076	0.0
25	5.165856272963845E-4	0.0	0.013302079902881903	0.0043909778320192685	0.0
26	5.165856272963845E-4	0.0	0.013302079902881903	0.0043909778320192685	0.0
27	5.165856272963845E-4	0.0	0.013431226309705998	0.005811588307084326	0.0
28	5.165856272963845E-4	0.0	0.013560372716530095	0.01201061583464094	0.0
29	5.165856272963845E-4	0.0	0.013560372716530095	0.024279524482930075	0.0
30	5.165856272963845E-4	0.0	0.013560372716530095	0.04287660706559992	0.0
31	5.165856272963845E-4	0.0	0.013560372716530095	0.09272712009970102	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCCC	25	0.00549525	29.599998	10
GGTATCA	615	0.0	27.07317	1
GTATCAA	1845	0.0	22.861786	1
CTCCGTT	45	0.0038246964	20.555557	36
AAGACGT	45	0.0038246964	20.555557	5
ATAGCGG	100	1.2882083E-8	20.349998	7
CGGGTAA	55	5.1410106E-4	20.181818	23
TTGGCGT	55	5.1410106E-4	20.181818	23
GCCGGCA	65	6.898413E-5	19.923077	15
GCCGCTC	65	6.898413E-5	19.923077	27
AGTTCGC	150	0.0	19.733332	8
CGGCCTT	235	0.0	19.680851	24
GTCGCCC	155	1.8189894E-12	19.096773	37
TCGGCCT	265	0.0	18.150944	23
TCGCCAT	245	0.0	18.122448	13
AGCGGCT	115	6.39684E-8	17.695652	9
AATAGCG	115	6.39684E-8	17.695652	6
GTATTAG	275	0.0	17.49091	1
AATCTCG	275	0.0	17.49091	10
TAGTTCG	150	2.5102054E-10	17.266666	7
>>END_MODULE