FastQCFastQC Report
Fri 10 Feb 2017
ERR1633455.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633455.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218414
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64530.5296229360463685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44470.36498267419776853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24110.19788019507326737No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC21090.17309387449586103No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14000.11490347287539376No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13200.10833756013965697No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12360.10144335176713334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAT250.00549647129.66
GGTATCA11600.028.2284491
GTATCAA30850.023.5072921
TACGGTA400.001931252923.12500212
TTAACGG3350.018.7761235
AACGGCC3450.017.69565237
TAACGGC3500.017.44285636
GCGAATC752.0673941E-417.26666833
GTATAAC1107.8085395E-716.8181821
AACGATT1554.0017767E-1016.70967722
GTGTTAT904.446308E-516.4444451
GCACCGC3950.016.39240510
TAGGACC2600.016.3653854
CTAATAC5550.016.0000023
CGGGTCA1851.8189894E-1116.017
GTTTAAC4050.015.98765533
CCGTCCG1403.4731784E-815.85714328
CGTCCCT700.002592612515.85714310
GGCACCG3850.015.8571429
GTATTAG6900.015.818841
Produced by FastQC (version 0.11.5)