##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633455.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1218414
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.895899915792167	33.0	33.0	33.0	33.0	33.0
2	32.00673990942323	33.0	33.0	33.0	33.0	33.0
3	31.730685957318283	33.0	33.0	33.0	27.0	33.0
4	32.007905358933826	33.0	33.0	33.0	33.0	33.0
5	32.10671413821574	33.0	33.0	33.0	33.0	33.0
6	35.27444037905014	37.0	37.0	37.0	33.0	37.0
7	35.4264560321861	37.0	37.0	37.0	33.0	37.0
8	35.55778741872631	37.0	37.0	37.0	33.0	37.0
9	35.666759410184056	37.0	37.0	37.0	33.0	37.0
10	35.68110839172892	37.0	37.0	37.0	33.0	37.0
11	35.69846456130675	37.0	37.0	37.0	33.0	37.0
12	35.682595570963564	37.0	37.0	37.0	33.0	37.0
13	35.68844087477655	37.0	37.0	37.0	33.0	37.0
14	35.6615033970391	37.0	37.0	37.0	33.0	37.0
15	35.67329003113884	37.0	37.0	37.0	33.0	37.0
16	35.6676236484479	37.0	37.0	37.0	33.0	37.0
17	35.65220360238802	37.0	37.0	37.0	33.0	37.0
18	35.63466112503632	37.0	37.0	37.0	33.0	37.0
19	35.63304755198151	37.0	37.0	37.0	33.0	37.0
20	35.634059523281906	37.0	37.0	37.0	33.0	37.0
21	35.63430164131404	37.0	37.0	37.0	33.0	37.0
22	35.54450786021828	37.0	37.0	37.0	33.0	37.0
23	35.58571881150414	37.0	37.0	37.0	33.0	37.0
24	35.582868384637734	37.0	37.0	37.0	33.0	37.0
25	35.6111116582705	37.0	37.0	37.0	33.0	37.0
26	35.500599960276226	37.0	37.0	37.0	33.0	37.0
27	35.51132784094733	37.0	37.0	37.0	33.0	37.0
28	35.53059715334853	37.0	37.0	37.0	33.0	37.0
29	35.54034671302201	37.0	37.0	37.0	33.0	37.0
30	35.53866830157894	37.0	37.0	37.0	33.0	37.0
31	35.530488815788395	37.0	37.0	37.0	33.0	37.0
32	35.51880149111878	37.0	37.0	37.0	33.0	37.0
33	35.51526574711059	37.0	37.0	37.0	33.0	37.0
34	35.49969140210142	37.0	37.0	37.0	33.0	37.0
35	35.44578936223648	37.0	37.0	37.0	33.0	37.0
36	35.47058142798753	37.0	37.0	37.0	33.0	37.0
37	35.46259809883997	37.0	37.0	37.0	33.0	37.0
38	35.456522988081225	37.0	37.0	37.0	33.0	37.0
39	35.35836997933379	37.0	37.0	37.0	33.0	37.0
40	35.14899369179934	37.0	37.0	37.0	33.0	37.0
41	35.326203572841415	37.0	37.0	37.0	33.0	37.0
42	35.362182312415975	37.0	37.0	37.0	33.0	37.0
43	35.037447862549186	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	8.0
15	30.0
16	69.0
17	61.0
18	49.0
19	46.0
20	83.0
21	201.0
22	496.0
23	1167.0
24	2407.0
25	4596.0
26	7313.0
27	11143.0
28	16095.0
29	21952.0
30	28384.0
31	36958.0
32	47472.0
33	62820.0
34	92082.0
35	188689.0
36	696293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3616389831371	20.17368480664208	13.851121211673536	24.61355499854729
2	16.161501755560916	21.32706945258344	39.103211223771225	23.40821756808441
3	17.830967142531193	27.358434817722056	29.841745088286903	24.968852951459848
4	12.480404854179286	17.984117057092252	39.6651712800411	29.870306808687356
5	13.943536433429033	37.00310403524582	35.0728898387576	13.980469692567551
6	29.128276595639907	39.770964548995664	17.559712872636066	13.541045982728367
7	26.551730364227595	32.32661476312649	23.309154359683983	17.812500512961932
8	24.746678879264355	35.44222243014279	20.491228761324148	19.319869929268705
9	26.37223472481439	15.47043944012462	20.141019390781786	38.0163064442792
10	15.53035339383822	27.88165598885108	33.20480559153128	23.383185025779415
11	33.5912095560294	24.079417997495103	24.08130569740663	18.248066749068872
12	23.605933615339286	25.643418411147607	29.538892363351042	21.211755610162065
13	27.77340050262062	22.342159561528348	26.08029782980169	23.80414210604934
14	22.3184402017705	20.95609538301431	27.81607893540291	28.909385479812283
15	23.99020365819828	28.298099004115183	24.92863673595346	22.783060601733073
16	22.827708808336084	28.1331304466298	25.12126420083814	23.91789654419598
17	22.426039096727386	27.182140060767523	26.355902016884247	24.035918825620847
18	23.304640294678165	26.77882887097489	27.694609549791778	22.22192128455517
19	25.232884717345666	25.672636722821636	27.43771821400608	21.656760345826623
20	24.34090547219582	26.115835832483867	27.498452906811643	22.04480578850867
21	23.62702661000284	26.60327277920313	27.398651033228443	22.37104957756559
22	23.118660816438418	26.540650386486035	27.38322113829946	22.957467658776082
23	22.894927339968188	26.567898924339346	28.11458174315134	22.422591992541125
24	23.244315971418583	26.695195557503443	26.847524732972538	23.212963738105437
25	23.265491039991332	26.46202358147559	27.85416122927018	22.418324149262894
26	23.45237333123224	26.67434878456748	27.71102433163112	22.162253552569158
27	23.414126889546573	26.656374598453397	26.812889543291522	23.1166089687085
28	22.638118078091683	26.372070576996	28.110478047691505	22.879333297220814
29	23.993732836293738	26.232216635724804	27.305004702834996	22.46904582514646
30	23.560792965281095	26.07988746025571	27.735564430480935	22.623755143982258
31	23.231266219856305	26.308052927822562	27.68541727196175	22.775263580359383
32	22.3208203451372	26.10943406756652	27.85900358991279	23.710741997383483
33	22.41487704507663	26.701515248511587	27.972183510695054	22.911424195716727
34	23.12809931599604	26.346955960781806	27.835038008427347	22.68990671479481
35	23.241771680233484	26.77956753615766	27.360240443724383	22.618420339884473
36	23.926186009024846	26.47630444167582	26.930993898625587	22.666515650673745
37	23.300290377490736	26.10409926346874	27.276032612888557	23.319577746151964
38	23.356182709653698	26.477289328586178	27.23737580165691	22.929152160103218
39	22.579517306925233	26.665402728465036	27.599650036851187	23.155429927758544
40	23.06317885382144	26.6837052102159	27.694609549791778	22.558506386170873
41	22.71649866137454	25.983286469131183	27.962580863319037	23.33763400617524
42	22.5255126746738	27.01938749883045	27.390115346671983	23.06498447982377
43	23.17553803551174	25.722045216158058	27.785629515090932	23.31678723323928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	72.5
2	86.0
3	390.0
4	694.0
5	694.0
6	807.5
7	921.0
8	857.0
9	793.0
10	1053.5
11	1314.0
12	1314.0
13	2205.5
14	3097.0
15	5534.5
16	7972.0
17	9161.5
18	10351.0
19	10351.0
20	9465.0
21	8579.0
22	9434.5
23	10290.0
24	13518.5
25	16747.0
26	16747.0
27	21093.5
28	25440.0
29	29737.0
30	34034.0
31	39405.5
32	44777.0
33	44777.0
34	54411.5
35	64046.0
36	69033.0
37	74020.0
38	79216.5
39	84413.0
40	84413.0
41	88405.0
42	92397.0
43	94397.0
44	96397.0
45	94950.5
46	93504.0
47	93504.0
48	89842.5
49	86181.0
50	86101.0
51	86021.0
52	83125.5
53	80230.0
54	80230.0
55	78433.5
56	76637.0
57	67170.0
58	57703.0
59	52460.0
60	47217.0
61	47217.0
62	42422.0
63	37627.0
64	30458.0
65	23289.0
66	19558.0
67	15827.0
68	15827.0
69	13402.5
70	10978.0
71	9526.0
72	8074.0
73	8185.0
74	8296.0
75	8296.0
76	7067.5
77	5839.0
78	4245.0
79	2651.0
80	1730.0
81	809.0
82	809.0
83	640.5
84	472.0
85	409.5
86	347.0
87	260.0
88	173.0
89	173.0
90	131.0
91	89.0
92	53.5
93	18.0
94	11.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1218414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.32766601699407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.56527226815292	41.22079841062624
2	9.405486442768835	9.279013879525333
3	2.509569112480148	3.7137356108095485
4	1.1033992318755044	2.177124351734507
5	0.647577531552494	1.597174409826543
6	0.41270753731455007	1.2214739738008946
7	0.27618289411154473	0.9536420292238781
8	0.2215384459837324	0.8742379578727546
9	0.18352007089007558	0.814735508785265
>10	1.3359471967019445	13.525039445887572
>50	0.18359089396069803	6.323277897826374
>100	0.14407386130762237	13.231014592006849
>500	0.009110056009109323	2.9986814172021523
>1k	0.0018557521500037512	1.5330440436170851
>5k	1.6870474090943194E-4	0.5370064712550782
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6453	0.5296229360463685	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4447	0.36498267419776853	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2411	0.19788019507326737	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2109	0.17309387449586103	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1400	0.11490347287539376	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1320	0.10833756013965697	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1236	0.10144335176713334	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.282956367868393E-4	0.0	0.0
2	8.207390919670982E-5	0.0	3.282956367868393E-4	0.0	0.0
3	8.207390919670982E-5	0.0	4.103695459835491E-4	8.207390919670982E-5	0.0
4	8.207390919670982E-5	0.0	6.565912735736786E-4	2.4622172759012945E-4	0.0
5	8.207390919670982E-5	0.0	8.207390919670982E-4	3.282956367868393E-4	0.0
6	1.6414781839341964E-4	0.0	0.0013131825471473571	3.282956367868393E-4	0.0
7	1.6414781839341964E-4	0.0	0.002872586821884844	3.282956367868393E-4	0.0
8	1.6414781839341964E-4	0.0	0.0034471041862618123	3.282956367868393E-4	0.0
9	2.4622172759012945E-4	0.0	0.00434991718742562	9.848869103605178E-4	0.0
10	4.103695459835491E-4	0.0	0.005334804097786138	9.848869103605178E-4	8.207390919670982E-5
11	4.103695459835491E-4	0.0	0.008125317010474272	0.0010669608195572277	8.207390919670982E-5
12	4.103695459835491E-4	0.0	0.00902813001163808	0.0010669608195572277	8.207390919670982E-5
13	4.103695459835491E-4	0.0	0.009684721285211759	0.0010669608195572277	8.207390919670982E-5
14	4.103695459835491E-4	0.0	0.010915829923162406	0.0010669608195572277	8.207390919670982E-5
15	4.103695459835491E-4	0.0	0.011818642924326214	0.0010669608195572277	8.207390919670982E-5
16	4.103695459835491E-4	0.0	0.012475234197899893	0.0011490347287539376	1.6414781839341964E-4
17	4.103695459835491E-4	0.0	0.012721455925490023	0.0013131825471473571	1.6414781839341964E-4
18	4.103695459835491E-4	0.0	0.013049751562276862	0.0013131825471473571	1.6414781839341964E-4
19	4.103695459835491E-4	0.0	0.01354219501745712	0.001395256456344067	1.6414781839341964E-4
20	4.103695459835491E-4	0.0	0.01362426892665383	0.0015594042747374866	1.6414781839341964E-4
21	4.924434551802589E-4	0.0	0.01387049065424396	0.0017235520931309061	1.6414781839341964E-4
22	4.924434551802589E-4	0.0	0.0141987862910308	0.0021339216391144554	1.6414781839341964E-4
23	4.924434551802589E-4	0.0	0.014362934109424219	0.0024622172759012945	1.6414781839341964E-4
24	4.924434551802589E-4	0.0	0.014445008018620928	0.0029546607310815534	1.6414781839341964E-4
25	4.924434551802589E-4	0.0	0.014445008018620928	0.003200882458671683	1.6414781839341964E-4
26	4.924434551802589E-4	0.0	0.014445008018620928	0.0038574737322453617	1.6414781839341964E-4
27	5.745173643769688E-4	0.0	0.014445008018620928	0.0048423606426058795	1.6414781839341964E-4
28	5.745173643769688E-4	0.0	0.014527081927817638	0.013788416745047249	1.6414781839341964E-4
29	5.745173643769688E-4	0.0	0.014609155837014349	0.029136237764831985	1.6414781839341964E-4
30	5.745173643769688E-4	0.0	0.014609155837014349	0.05490744525259887	1.6414781839341964E-4
31	5.745173643769688E-4	0.0	0.014609155837014349	0.1127695512362793	1.6414781839341964E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGAT	25	0.005496471	29.6	6
GGTATCA	1160	0.0	28.228449	1
GTATCAA	3085	0.0	23.507292	1
TACGGTA	40	0.0019312529	23.125002	12
TTAACGG	335	0.0	18.77612	35
AACGGCC	345	0.0	17.695652	37
TAACGGC	350	0.0	17.442856	36
GCGAATC	75	2.0673941E-4	17.266668	33
GTATAAC	110	7.8085395E-7	16.818182	1
AACGATT	155	4.0017767E-10	16.709677	22
GTGTTAT	90	4.446308E-5	16.444445	1
GCACCGC	395	0.0	16.392405	10
TAGGACC	260	0.0	16.365385	4
CTAATAC	555	0.0	16.000002	3
CGGGTCA	185	1.8189894E-11	16.0	17
GTTTAAC	405	0.0	15.987655	33
CCGTCCG	140	3.4731784E-8	15.857143	28
CGTCCCT	70	0.0025926125	15.857143	10
GGCACCG	385	0.0	15.857142	9
GTATTAG	690	0.0	15.81884	1
>>END_MODULE