FastQCFastQC Report
Fri 10 Feb 2017
ERR1633454.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633454.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028345
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT232112.2571218803028166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT164241.5971293680622747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94720.9210916569828219No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA57630.5604150358099665No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA53210.5174333516475502No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG46170.4489738366015296No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA40010.38907176093626167No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39500.38411233584059823No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG38580.3751659219425387No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT36120.35124398912816224No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC31470.30602570149123104No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT30310.29474544048932994No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT28780.2798671652023397No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC26630.2589597848970919No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC25290.2459291385673096No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG24740.24058073895433926No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA24390.2371772119279036No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC24330.23659375015194317No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT24230.23562131385867582No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG23970.23309297949618077No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA23820.23163432505627973No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC23820.23163432505627973No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.231537081426953No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC23320.22677214358994305No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA22130.21520015170006174No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA21400.20810136675921018No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA21040.20460059610344777No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA20690.2011970690770121No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG19910.19361206598952685No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA19630.1908892443683783No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19560.19020853896309117No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC19420.18884712815251692No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA19220.18690225556598222No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG19170.18641603741934856No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG19170.18641603741934856No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC19120.1859298192727149No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC19000.1847628957207941No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT18800.1828180231342594No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT18670.18155385595301188No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT18310.17805308529724947No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC18160.17659443085734847No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG17540.17056532583909098No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG16970.16502243896746716No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA16650.16191064282901166No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG16580.16122993742372455No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC16580.16122993742372455No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA16530.16074371927709086No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG16330.1587988466905562No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT16040.15597878144008093No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG16010.15568705055210072No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG15860.1542283961121997No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA15820.15383942159489275No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT15710.1527697416722987No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG15460.15033865093913035No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG15440.1501441636804769No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA15270.1484910219819224No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT15080.1466433930247145No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG14800.14392057140356593No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14670.1426564042223184No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14640.1423646733343382No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT14560.1415867242997243No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14020.13633556831608068No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13500.13127889959109054No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC13460.1308899250737836No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC13400.1303064632978232No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13110.12748639804734793No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG13100.12738915441802118No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13060.12700017990071424No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT12880.12524979457283306No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT12760.12408287102091224No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC12700.12349940924495184No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC12620.12272146021033796No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA12600.1225269729516845No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA12300.1196096640718825No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT12240.1190262022959221No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA12090.1175675478560211No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT11900.11571991889881314No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC11890.11562267526948641No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC11840.11513645712285274No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG11640.11319158453631806No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA11620.1129970972776646No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA11480.11163568646709032No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA11390.11076049380314971No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG11370.11056600654449625No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG11320.11007978839786259No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC11320.11007978839786259No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT11120.1081349158113279No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG11000.1069679922594071No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG10960.10657901774210017No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10880.1058010687074863No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG10870.10570382507815958No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT10840.10541209419017937No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10830.10531485056085263No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC10820.1052176069315259No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG10760.10463414515556549No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG10750.10453690152623876No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG10500.1021058107930704No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG10470.10181407990509021No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC10460.10171683627576349No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA10420.10132786175845655No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG10310.10025818183586248No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA10300.10016093820653575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGT200.001841845937.04
CCTATAG303.599217E-430.8333343
AACAAAG250.00549607829.59999829
TTTACGA250.00549607829.5999985
CTAGTGC502.7203168E-729.5999989
AAACCCC250.00549607829.59999837
AACAGCG250.00549607829.59999823
TGCACTG250.00549607829.5999985
CAGACCT250.00549607829.59999837
GTTTCAA405.939531E-527.751
TAACAAC556.2606705E-726.909091
TGTCAAC358.868757E-426.4285722
TGGAACC358.868757E-426.42857224
TATAGTG509.089874E-625.8999985
GAATCAA659.389805E-825.6153852
TAGTGCA601.3371773E-624.66666810
AACATAG551.9024066E-523.54545431
TCGTATT551.9024066E-523.54545414
ACATAGC551.9024066E-523.54545432
AACCGCT400.001931049623.12527
Produced by FastQC (version 0.11.5)