FastQCFastQC Report
Fri 10 Feb 2017
ERR1633453.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633453.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986401
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25730.2608472619147791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17290.17528368280243026No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC15210.15419692396905518No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12660.12834536866852325No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG12530.1270274462414373No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA12310.12479711598021495No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11550.11709233871417404No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11100.11253029954349195No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10170.1031020852574156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10110.10249381336799131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG2900.022.32758535
GTATCAA18650.022.0214461
TCGCGAG450.003825428720.55555722
AACGGCC3200.020.23437537
TTACTCG555.142383E-420.18181819
CGGATCG555.142383E-420.18181837
TAACGGC3300.019.62121236
GGTATCA7450.019.1208061
TTTAACG3400.019.04411734
CTATCGC801.6162918E-518.532
CTCGCGA500.007034193318.49999821
GTTTAAC3850.017.7792233
CGAACTA2950.017.55932229
ACGTAGG2900.017.22413827
TCACGTA2900.017.22413825
GCGAACT3050.016.98360628
TATTAGA4500.016.8555562
TATTGCG904.444927E-516.44444511
TATCGCC904.444927E-516.44444533
TTTTCGG1352.2180757E-816.44444326
Produced by FastQC (version 0.11.5)