##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633453.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	986401
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.849331052989605	33.0	33.0	33.0	27.0	33.0
2	31.96883316217238	33.0	33.0	33.0	33.0	33.0
3	31.675092584050503	33.0	33.0	33.0	27.0	33.0
4	31.960027412786484	33.0	33.0	33.0	33.0	33.0
5	32.05765099589315	33.0	33.0	33.0	33.0	33.0
6	35.23405896790453	37.0	37.0	37.0	33.0	37.0
7	35.37511113634313	37.0	37.0	37.0	33.0	37.0
8	35.50253801445862	37.0	37.0	37.0	33.0	37.0
9	35.62241623842636	37.0	37.0	37.0	33.0	37.0
10	35.6253876466062	37.0	37.0	37.0	33.0	37.0
11	35.647338151522554	37.0	37.0	37.0	33.0	37.0
12	35.641446024486996	37.0	37.0	37.0	33.0	37.0
13	35.6335891792486	37.0	37.0	37.0	33.0	37.0
14	35.61738278854138	37.0	37.0	37.0	33.0	37.0
15	35.63370170954815	37.0	37.0	37.0	33.0	37.0
16	35.62034304506991	37.0	37.0	37.0	33.0	37.0
17	35.614281615691795	37.0	37.0	37.0	33.0	37.0
18	35.58877981672768	37.0	37.0	37.0	33.0	37.0
19	35.58462227836346	37.0	37.0	37.0	33.0	37.0
20	35.58209592244939	37.0	37.0	37.0	33.0	37.0
21	35.58443979679664	37.0	37.0	37.0	33.0	37.0
22	35.49031884598657	37.0	37.0	37.0	33.0	37.0
23	35.54242848496707	37.0	37.0	37.0	33.0	37.0
24	35.52249642893712	37.0	37.0	37.0	33.0	37.0
25	35.55289380282461	37.0	37.0	37.0	33.0	37.0
26	35.44792533665315	37.0	37.0	37.0	33.0	37.0
27	35.46124750481802	37.0	37.0	37.0	33.0	37.0
28	35.477926320026036	37.0	37.0	37.0	33.0	37.0
29	35.48399079076359	37.0	37.0	37.0	33.0	37.0
30	35.491897311539624	37.0	37.0	37.0	33.0	37.0
31	35.47433650209195	37.0	37.0	37.0	33.0	37.0
32	35.475261075363875	37.0	37.0	37.0	33.0	37.0
33	35.47280973964949	37.0	37.0	37.0	33.0	37.0
34	35.45971567344316	37.0	37.0	37.0	33.0	37.0
35	35.3978199535483	37.0	37.0	37.0	33.0	37.0
36	35.436718940876986	37.0	37.0	37.0	33.0	37.0
37	35.4336927882271	37.0	37.0	37.0	33.0	37.0
38	35.4257730882268	37.0	37.0	37.0	33.0	37.0
39	35.327228986994136	37.0	37.0	37.0	33.0	37.0
40	35.13524621325404	37.0	37.0	37.0	33.0	37.0
41	35.307700417984165	37.0	37.0	37.0	33.0	37.0
42	35.351736261419035	37.0	37.0	37.0	33.0	37.0
43	35.018336356106694	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	23.0
16	54.0
17	30.0
18	21.0
19	26.0
20	64.0
21	145.0
22	444.0
23	995.0
24	2191.0
25	3825.0
26	6349.0
27	9626.0
28	13472.0
29	18390.0
30	24108.0
31	30544.0
32	39437.0
33	51865.0
34	75739.0
35	154556.0
36	554494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.86948411447271	20.573073222756264	13.640699877635972	24.916742785135053
2	16.242177370055384	21.09709945549528	38.53990415662595	24.12081901782338
3	17.87954391773731	26.849323956484227	29.50078112248467	25.77035100329379
4	12.61454520017721	17.823278767965565	39.207077040676154	30.355098991181073
5	13.954466793930663	37.05612626102366	35.30896663730066	13.680440307745027
6	29.9833434880946	39.48242144928888	17.272184436147164	13.262050626469357
7	27.19401136049132	31.666431806131584	23.41228364529233	17.727273188084762
8	24.6833691368926	35.68153316957302	20.30310188250012	19.331995811034254
9	25.997439175345523	15.508297335464988	20.10450111060309	38.3897623785864
10	15.369104451435064	27.680933007975455	33.528453438307544	23.42150910228193
11	34.175857485951454	23.443406890301205	23.88977707849039	18.490958545256948
12	23.364331544676052	25.72138511619514	29.615136237696433	21.29914710143238
13	27.927181744544054	22.124774812677604	25.699791464120576	24.248251978657766
14	22.139677473968497	20.818916444731908	27.280385968789567	29.76102011251002
15	24.325198372669938	28.3200240064639	24.080064801231956	23.274712819634207
16	23.415629140684164	28.054310569433728	24.796203572380808	23.7338567175013
17	22.759202393347127	27.34344348799322	26.031401022505047	23.86595309615461
18	23.31090499705495	27.157515047125862	27.155284716864642	22.376295238954544
19	25.048839163788355	26.31931638349921	26.76609208628134	21.865752366431096
20	24.40508474748099	26.212767424201722	26.914409048652626	22.467738779664657
21	23.514878837308558	26.809178011782226	27.00179744343325	22.674145707475965
22	23.08645266985739	26.742673618538504	26.83036614926384	23.340507562340264
23	23.037993675999925	26.601757297488547	27.43245394114564	22.92779508536589
24	23.320434589989265	27.393321782926012	25.697865269804065	23.58837835728066
25	23.376801118409247	26.842227451107615	26.93407650641068	22.846894924072462
26	23.414412596905315	27.046404048657696	26.91846419458212	22.620719159854865
27	23.214392523932965	26.91420629135615	26.251088553235448	23.620312631475436
28	22.877409897191914	26.59608009318725	27.315868495672653	23.210641513948183
29	24.468851917222306	26.565463741419563	26.17870419839396	22.78698014296417
30	23.77217784653503	26.336753510996036	26.90710978597954	22.9839588564894
31	23.574894997065087	26.42870394494734	26.771667911934394	23.22473314605318
32	22.51569088028094	26.276737351239504	27.206176798279806	24.001394970199748
33	22.573780845720957	26.42322949794252	27.674748910432978	23.328240745903543
34	23.581585987848754	26.1337934572248	27.13987516233256	23.144745392593887
35	23.31445324974326	26.91886970917507	26.799547040199673	22.967130000881994
36	24.356118860382338	26.477061560156567	26.262138825893324	22.904680753567767
37	23.44715790028599	26.17708214002216	26.496627639266386	23.879132320425466
38	23.482437669872596	26.687422255249132	26.51558544648677	23.314554628391495
39	22.88764914066389	26.49561385278401	26.91228009703964	23.704456909512462
40	23.154072228231723	27.13328555019713	27.11696358783091	22.59567863374023
41	23.13065376048889	26.102974348160636	27.0323124165527	23.734059474797775
42	22.826315058480272	27.042551660024678	26.91471318459734	23.21642009689771
43	23.680328791231965	25.8473987759542	26.748249444191565	23.724022988622274
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	30.5
2	44.0
3	120.0
4	196.0
5	196.0
6	260.0
7	324.0
8	317.0
9	310.0
10	420.5
11	531.0
12	531.0
13	877.0
14	1223.0
15	2297.0
16	3371.0
17	3979.5
18	4588.0
19	4588.0
20	4402.0
21	4216.0
22	4912.0
23	5608.0
24	7649.0
25	9690.0
26	9690.0
27	13477.0
28	17264.0
29	21143.5
30	25023.0
31	30315.5
32	35608.0
33	35608.0
34	43633.0
35	51658.0
36	56974.0
37	62290.0
38	66833.0
39	71376.0
40	71376.0
41	75187.0
42	78998.0
43	81240.5
44	83483.0
45	81775.0
46	80067.0
47	80067.0
48	77250.0
49	74433.0
50	73743.0
51	73053.0
52	69987.0
53	66921.0
54	66921.0
55	64963.0
56	63005.0
57	54434.5
58	45864.0
59	42672.0
60	39480.0
61	39480.0
62	34554.5
63	29629.0
64	25112.0
65	20595.0
66	17306.0
67	14017.0
68	14017.0
69	11637.5
70	9258.0
71	7499.0
72	5740.0
73	4678.5
74	3617.0
75	3617.0
76	2825.0
77	2033.0
78	1594.5
79	1156.0
80	930.5
81	705.0
82	705.0
83	580.5
84	456.0
85	374.5
86	293.0
87	234.5
88	176.0
89	176.0
90	118.0
91	60.0
92	40.5
93	21.0
94	12.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	986401.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.63175364590718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14297947553345	48.21796238336941
2	8.693995953143109	9.84712474033829
3	2.3545683267500546	4.00030000268895
4	1.0312482998124535	2.3360559865095794
5	0.5944949733233899	1.6833646436490186
6	0.3619508421524976	1.2298746554825328
7	0.25762077071689793	1.0212661214915673
8	0.1980055374301087	0.8970720653013896
9	0.14899983497456706	0.7594309752804456
>10	0.992255233140979	11.016641802918341
>50	0.11910479885439872	4.748821701562578
>100	0.09625611024501814	10.260587954446452
>500	0.006707111173631693	2.558037887343877
>1k	0.0018127327496301872	1.4234590796175537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2573	0.2608472619147791	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1729	0.17528368280243026	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1521	0.15419692396905518	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1266	0.12834536866852325	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1253	0.1270274462414373	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1231	0.12479711598021495	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1155	0.11709233871417404	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1110	0.11253029954349195	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1017	0.1031020852574156	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1011	0.10249381336799131	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.0275729647476027E-4	0.0	0.0
4	0.0	0.0	2.0275729647476027E-4	0.0	0.0
5	1.0137864823738013E-4	0.0	2.0275729647476027E-4	0.0	0.0
6	1.0137864823738013E-4	0.0	8.110291858990411E-4	0.0	0.0
7	1.0137864823738013E-4	0.0	0.0022303302612223627	1.0137864823738013E-4	0.0
8	1.0137864823738013E-4	0.0	0.0027372235024092635	1.0137864823738013E-4	0.0
9	1.0137864823738013E-4	0.0	0.0033454953918335443	6.082718894242808E-4	0.0
10	2.0275729647476027E-4	0.0	0.0034468740400709244	7.096505376616609E-4	1.0137864823738013E-4
11	2.0275729647476027E-4	0.0	0.005068932411869007	7.096505376616609E-4	1.0137864823738013E-4
12	2.0275729647476027E-4	0.0	0.005271689708343767	8.110291858990411E-4	1.0137864823738013E-4
13	2.0275729647476027E-4	0.0	0.0055758256530559075	8.110291858990411E-4	1.0137864823738013E-4
14	2.0275729647476027E-4	0.0	0.005879961597768047	8.110291858990411E-4	1.0137864823738013E-4
15	2.0275729647476027E-4	0.0	0.006082718894242808	9.124078341364212E-4	2.0275729647476027E-4
16	2.0275729647476027E-4	0.0	0.006386854838954948	9.124078341364212E-4	2.0275729647476027E-4
17	2.0275729647476027E-4	1.0137864823738013E-4	0.006386854838954948	0.0010137864823738013	2.0275729647476027E-4
18	2.0275729647476027E-4	1.0137864823738013E-4	0.006690990783667089	0.0012165437788485617	2.0275729647476027E-4
19	2.0275729647476027E-4	1.0137864823738013E-4	0.006995126728379229	0.0012165437788485617	2.0275729647476027E-4
20	2.0275729647476027E-4	1.0137864823738013E-4	0.007096505376616609	0.0012165437788485617	3.041359447121404E-4
21	2.0275729647476027E-4	1.0137864823738013E-4	0.007197884024853989	0.0012165437788485617	3.041359447121404E-4
22	2.0275729647476027E-4	1.0137864823738013E-4	0.00729926267309137	0.0016220583717980821	3.041359447121404E-4
23	2.0275729647476027E-4	1.0137864823738013E-4	0.00729926267309137	0.0021289516129849827	3.041359447121404E-4
24	2.0275729647476027E-4	1.0137864823738013E-4	0.0074006413213287495	0.0024330875576971233	3.041359447121404E-4
25	2.0275729647476027E-4	1.0137864823738013E-4	0.0074006413213287495	0.0025344662059345034	3.041359447121404E-4
26	2.0275729647476027E-4	1.0137864823738013E-4	0.0074006413213287495	0.0028386021506466436	3.041359447121404E-4
27	2.0275729647476027E-4	1.0137864823738013E-4	0.0074006413213287495	0.0035482526883083045	3.041359447121404E-4
28	2.0275729647476027E-4	1.0137864823738013E-4	0.0074006413213287495	0.009124078341364212	3.041359447121404E-4
29	2.0275729647476027E-4	1.0137864823738013E-4	0.0074006413213287495	0.020884001536900308	3.041359447121404E-4
30	2.0275729647476027E-4	1.0137864823738013E-4	0.0074006413213287495	0.035583905531320424	3.041359447121404E-4
31	2.0275729647476027E-4	1.0137864823738013E-4	0.0074006413213287495	0.08181256912756577	3.041359447121404E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	290	0.0	22.327585	35
GTATCAA	1865	0.0	22.021446	1
TCGCGAG	45	0.0038254287	20.555557	22
AACGGCC	320	0.0	20.234375	37
TTACTCG	55	5.142383E-4	20.181818	19
CGGATCG	55	5.142383E-4	20.181818	37
TAACGGC	330	0.0	19.621212	36
GGTATCA	745	0.0	19.120806	1
TTTAACG	340	0.0	19.044117	34
CTATCGC	80	1.6162918E-5	18.5	32
CTCGCGA	50	0.0070341933	18.499998	21
GTTTAAC	385	0.0	17.77922	33
CGAACTA	295	0.0	17.559322	29
ACGTAGG	290	0.0	17.224138	27
TCACGTA	290	0.0	17.224138	25
GCGAACT	305	0.0	16.983606	28
TATTAGA	450	0.0	16.855556	2
TATTGCG	90	4.444927E-5	16.444445	11
TATCGCC	90	4.444927E-5	16.444445	33
TTTTCGG	135	2.2180757E-8	16.444443	26
>>END_MODULE