FastQCFastQC Report
Fri 10 Feb 2017
ERR1633452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267707
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46110.36372758058447263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30880.24358940985574742No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC19710.1554775669772274No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA18790.14822036953333853No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC18180.1434085320977166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18040.1423041759649509No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT16960.13378485722647268No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT14020.11059337843839309No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13610.10735919262100785No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA13140.10365171131815158No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA12950.10215294228082673No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC12790.10089082098623736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG250.00549655529.5999986
ATACGCG250.00549655529.5999985
GGTATCA11200.024.6116071
GTATCAA31350.023.78151
CGCCTAT551.9028597E-523.54545436
CTATACG450.003826022120.5555553
GCGTTAT450.003826022120.55555511
TCTATCG701.219323E-418.531
TCGCCAT2850.018.17543813
TTCGCCG1054.798221E-717.61904724
ATTAGAC650.001580055217.0769233
TATACGG650.001580055217.07692332
GTACTAT1950.017.0769211
TTAACGG2550.016.68627435
GTCGCCA2900.016.58620612
TCAACGC45900.016.2832244
ATCAACG45350.016.2767373
ATCTCGC2850.016.2280711
AACGCAG46600.016.1180256
CAACGCA46400.016.1077585
Produced by FastQC (version 0.11.5)