FastQCFastQC Report
Fri 10 Feb 2017
ERR1633451.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633451.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779272
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT188842.4232873759098235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT142151.8241384266340894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76700.984251968503937No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA46060.5910644806948023No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA38280.49122770996519827No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34100.43758790255520535No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG33340.427835210298843No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT31760.4075598763974581No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA29240.37522200207373035No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG27790.35661489184777584No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC26380.33852108121426155No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT22460.2882177211551294No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.2635793407180035No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT20280.2602428933671427No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC19870.2549815725446314No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC18900.24253405742795842No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18890.24240573252984837No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC18600.23868431048465746No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG18510.2375293864016672No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18390.23598948762434685No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC18220.2338079643564763No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA18170.23316633986592614No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC17570.22546684597932432No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA17210.22084714964736318No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT17090.2193072508700428No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA16850.21622745331540205No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG16630.2134043055569814No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16180.20762968514202998No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC15520.19916024186676795No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA15470.19851861737621782No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA15310.1964654190064573No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA15260.19582379451590715No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC15170.1946688704329169No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC14440.1853011528708846No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG14320.18376125409356425No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT14180.18196470552002383No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14180.18196470552002383No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC14170.1818363806219138No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG14140.1814514059275837No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT13890.17824328347483293No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC13810.1772166842899527No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG13510.17336693734665173No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG12980.1665657177468201No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT12880.16528246876571978No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG12640.16220267121107906No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12630.16207434631296902No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG12420.15937952345265838No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT12390.1589945487583283No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA12380.15886622386021826No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC12190.15642805079612768No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG11860.15219332915849665No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG11790.15129505487172643No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11750.1507817552792863No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA11460.1470603332340954No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT11390.1461620589473252No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG11270.14462216017000482No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA11250.14436551037378476No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG11230.1441088605775647No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11200.14372388588323462No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG11160.1432105862907945No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG11100.1424406369021343No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT11010.14128571281914404No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10690.13717931607962303No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC10640.1365376915890729No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG10610.1361527168947428No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA10570.13563941730230267No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT10480.13448449321931238No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10460.13422784342309232No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA10340.13268794464577197No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10240.13140469566467164No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG10150.13024977158168138No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG10030.128709872804361No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT10010.12845322300814094No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG9990.12819657321192088No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC9920.12729829892515065No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9820.12601504994405036No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9570.12280692749129957No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG9540.12242195279696948No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA9440.12113870381586916No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG9320.11959880503854879No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT9300.11934215524232873No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC9060.116262357687688No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA8860.11369585972548737No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT8840.11343920992926732No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG8770.1125409356424971No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG8710.11177098625383693No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8620.11061606217084663No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC8620.11061606217084663No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT8590.11023108747651655No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.10907616339352626No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC8480.1088195135973062No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC8460.10856286380108614No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG8400.10779291441242597No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8380.1075362646162059No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG8320.10676631522754572No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA8250.10586804094077548No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG8230.10561139114455542No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG8190.1050980915521153No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA8060.1034298678766849No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC7980.10240326869180466No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7840.10060672011826421No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA7820.10035007032204415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATAC200.001841572637.05
AGATGTC303.5984238E-430.8333345
GTATTAT502.7188798E-729.61
AGACAAG250.005495270729.623
AGAGGAG250.005495270729.65
ACAAGTG250.005495270729.625
CTCCACT454.0060786E-628.7777792
TTATAGA454.0060786E-628.7777794
TATTATA454.0060786E-628.7777792
TTGCACT405.9377133E-527.754
CTAGTGG556.2573963E-726.909099
ATGTCAC358.86682E-426.4285727
CTAGTGC706.5847416E-926.4285729
ACCAAGA358.86682E-426.42857212
ATTATAG509.086183E-625.9000023
GACATCT451.3226776E-424.66666818
TAAGGAT601.3364825E-624.66666824
ACTAGTG1450.024.2413798
ATAGATC551.9016401E-523.5454546
TATAGAT551.9016401E-523.5454545
Produced by FastQC (version 0.11.5)