FastQCFastQC Report
Fri 10 Feb 2017
ERR1633450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262078
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT188101.4903991670879297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT135891.0767163360743155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72400.573657095678714No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA47530.37660112924874695No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA43090.34142105321541144No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT38230.3029131321518955No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG35700.2828668275653327No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33790.26773305611856No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC33190.2629789917897309No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG33130.262503585356848No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA32720.2592549747321481No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA25270.2002253426491865No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT24600.19491663748199398No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA24490.19404505902170863No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT22060.1747910984899507No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC21740.17225559751457517No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG20860.16528296983229246No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC20110.15934038942125606No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC20080.1591026862048146No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20000.15846881096097074No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG19770.15664641963491957No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC19670.1558540755801147No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC19460.1541901530650245No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC19340.15323934019925867No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC19220.15228852733349285No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18950.15014919838551977No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.14753446300466375No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC18590.1472967597882223No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA18030.1428596330813151No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC17830.1412749449717054No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA17590.13937331924017374No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA17070.13525313015518853No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA17010.1347777237223056No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA16320.1293105497441521No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA15940.12629964233589366No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC15760.12487342303724493No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15730.12463571982080346No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT15660.12408107898244007No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT15600.12360567254955715No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG15470.12257562527831084No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG15390.12194175003446697No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA15370.12178328122350598No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG15290.1211494059796621No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG15220.12059476514129872No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC14860.11774232654400124No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC14800.11726692011111833No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC14790.11718768570563784No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG14750.1168707480837159No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG14400.11409754389189891No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC13910.11021505802335514No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT13890.11005658921239415No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG13870.10989812040143318No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA13870.10989812040143318No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13840.10966041718499173No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT13770.10910577634662834No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT13770.10910577634662834No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG13760.10902654194114786No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG13640.10807572907538202No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT13530.1072041506150967No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA13480.10680797858769427No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC13470.10672874418221377No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG13350.10577793131644796No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT13260.10506482166712358No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG13220.10474788404520165No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG13070.10355936796299436No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT13050.10340089915203338No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA13020.10316319593559195No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC12930.10245008628626756No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG12650.10023152293281397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT308.312634E-637.019
GCTCTAG401.6002323E-632.3751
AGCGGAA401.6002323E-632.37518
CCGGCCA303.5996767E-430.83333425
CGCCAAC303.5996767E-430.83333415
AGCAACG250.005496544329.62
TATGCTG405.940583E-527.75000232
CGTAGCG405.940583E-527.75000215
GGTATGC405.940583E-527.75000230
ACAACGC556.2625804E-726.909093
TGTTCGA358.8698795E-426.4285737
GATTGGG358.8698795E-426.428572
GCCAACT358.8698795E-426.4285716
TTGATTC451.3233123E-424.66666824
CTGTACT751.3740646E-824.6666684
GGTATCA34950.024.6666681
GCCGCTC8550.024.45029327
GCTCTCT8350.024.37125830
CGCTCTC8350.024.37125829
CCGCTCT8500.024.15882528
Produced by FastQC (version 0.11.5)