##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1633450.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1262078 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9183061585734 33.0 33.0 33.0 33.0 33.0 2 32.017733452290585 33.0 33.0 33.0 33.0 33.0 3 31.72925603647318 33.0 33.0 33.0 27.0 33.0 4 32.006120065479315 33.0 33.0 33.0 33.0 33.0 5 32.116631460179164 33.0 33.0 33.0 33.0 33.0 6 35.253040620310315 37.0 37.0 37.0 33.0 37.0 7 35.413739087441506 37.0 37.0 37.0 33.0 37.0 8 35.55163310033136 37.0 37.0 37.0 33.0 37.0 9 35.66099321911958 37.0 37.0 37.0 33.0 37.0 10 35.6293604674196 37.0 37.0 37.0 33.0 37.0 11 35.65127828866362 37.0 37.0 37.0 33.0 37.0 12 35.64895751292709 37.0 37.0 37.0 33.0 37.0 13 35.64426525143454 37.0 37.0 37.0 33.0 37.0 14 35.629525274983 37.0 37.0 37.0 33.0 37.0 15 35.67039834305011 37.0 37.0 37.0 33.0 37.0 16 35.66144960929515 37.0 37.0 37.0 33.0 37.0 17 35.64433735474353 37.0 37.0 37.0 33.0 37.0 18 35.62469831500113 37.0 37.0 37.0 33.0 37.0 19 35.598780740968465 37.0 37.0 37.0 33.0 37.0 20 35.596145404642186 37.0 37.0 37.0 33.0 37.0 21 35.586407496208636 37.0 37.0 37.0 33.0 37.0 22 35.486139525449296 37.0 37.0 37.0 33.0 37.0 23 35.53640583228612 37.0 37.0 37.0 33.0 37.0 24 35.53327765795775 37.0 37.0 37.0 33.0 37.0 25 35.54744160028144 37.0 37.0 37.0 33.0 37.0 26 35.44679488906391 37.0 37.0 37.0 33.0 37.0 27 35.44889935487347 37.0 37.0 37.0 33.0 37.0 28 35.47167924644911 37.0 37.0 37.0 33.0 37.0 29 35.47338674788721 37.0 37.0 37.0 33.0 37.0 30 35.46967699302262 37.0 37.0 37.0 33.0 37.0 31 35.43787943375924 37.0 37.0 37.0 33.0 37.0 32 35.42268782119647 37.0 37.0 37.0 33.0 37.0 33 35.408231503916554 37.0 37.0 37.0 33.0 37.0 34 35.36363758816808 37.0 37.0 37.0 33.0 37.0 35 35.28773578178211 37.0 37.0 37.0 33.0 37.0 36 35.288101052391376 37.0 37.0 37.0 33.0 37.0 37 35.253312394321114 37.0 37.0 37.0 33.0 37.0 38 35.21276418731647 37.0 37.0 37.0 33.0 37.0 39 35.099220491918885 37.0 37.0 37.0 33.0 37.0 40 34.85432754552413 37.0 37.0 37.0 27.0 37.0 41 34.98234657445895 37.0 37.0 37.0 27.0 37.0 42 34.95528406326709 37.0 37.0 37.0 27.0 37.0 43 34.5719392937679 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 5.0 15 47.0 16 56.0 17 49.0 18 59.0 19 49.0 20 91.0 21 213.0 22 600.0 23 1393.0 24 2699.0 25 4766.0 26 8107.0 27 12412.0 28 17693.0 29 24581.0 30 32328.0 31 42136.0 32 52870.0 33 69301.0 34 99461.0 35 196732.0 36 696430.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.005063078510204 18.43047735559926 14.09310676519201 22.47135280069853 2 17.037298804035885 22.485060352846656 38.79926597246763 21.678374870649833 3 18.8619086934405 28.915090826399002 29.79934679156122 22.42365368859928 4 11.568460903367304 18.413838130448358 40.724424322426984 29.29327664375736 5 14.339129594208917 36.28737684992529 33.135590668722536 16.237902887143267 6 26.333079255006425 41.79424726522449 16.46245319227496 15.410220287494116 7 26.183801635081196 33.25206524477885 21.17198778522405 19.39214533491591 8 25.47013734491846 32.46558453597955 19.415836422154573 22.648441696947415 9 28.76914105150395 13.17232373910329 18.595047215782227 39.463487993610535 10 17.511992127269473 25.8585444005838 30.61934365387876 26.010119818267967 11 34.93753951815973 24.37297853223018 21.80855699885427 18.88092495075582 12 25.04615404119238 26.518487763830763 27.306473926334192 21.12888426864267 13 31.0597284795393 22.883450943602533 24.070144634483764 21.9866759423744 14 23.933386050624446 21.358980982157995 28.631352420373386 26.07628054684417 15 26.719109278507354 26.014953117002275 26.30978433979516 20.95615326469521 16 21.18331830520776 27.386421441463998 25.500484122217486 25.929776131110753 17 22.90365571700006 25.097497935943736 24.549750490857143 27.44909585619906 18 25.612759274783333 22.782664779831357 28.079088614174402 23.525487331210908 19 28.37249361766864 23.232874671771476 28.472962843817896 19.921668866741992 20 27.72998182362738 22.162180150513677 28.174645307183866 21.933192718675073 21 24.070303103294723 25.33591426203452 28.443963051412034 22.14981958325872 22 23.628571292741018 24.95772844467616 28.41686488473771 22.99683537784511 23 23.291191194205112 24.861379407611892 29.805051668755816 22.04237772942718 24 23.23834184574963 25.187508220569573 28.003657460156976 23.570492473523824 25 23.31646696955339 25.48519188195975 29.733503000606937 21.464838147879924 26 23.660423523744175 25.540497496985132 28.504260433982687 22.29481854528801 27 24.017057582811837 25.13759054511686 28.513768562640347 22.331583309430954 28 22.366367213436888 25.408889149482043 28.671128091924587 23.553615545156482 29 24.48897770185361 25.058118436419935 27.29593575040528 23.15696811132117 30 24.405068466449777 24.260069504420485 29.27505273049685 22.05980929863289 31 23.828638166579243 24.51211414825391 28.558377532925856 23.100870152240986 32 21.36468585935259 24.548324271558496 29.56386213847322 24.5231277306157 33 21.75951090186185 24.83951071169928 29.967402965585325 23.433575420853543 34 22.10259587759235 24.991165363788927 29.233930074052477 23.67230868456625 35 22.654859683791333 25.817738681761348 28.324636036758427 23.20276559768889 36 23.596402124115944 25.219281217167243 28.642841409168057 22.54147524954876 37 23.82126936686956 25.07325220786671 28.131779493818925 22.973698931444808 38 23.255931883766294 25.51284468947244 27.300610580328634 23.93061284643263 39 22.603119617012577 24.8040929324495 28.4709027492754 24.121884701262523 40 22.671498908942237 26.357087279867013 28.701395634818134 22.270018176372616 41 23.48238381462952 24.659490142447616 28.22598920193522 23.63213684098764 42 21.833832774202545 26.623552585497883 28.276382283820812 23.26623235647876 43 22.972906587390003 25.699045542351584 27.678637928876025 23.649409941382388 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 201.0 1 209.5 2 218.0 3 922.5 4 1627.0 5 1627.0 6 2010.5 7 2394.0 8 2405.0 9 2416.0 10 3406.5 11 4397.0 12 4397.0 13 6863.0 14 9329.0 15 16178.5 16 23028.0 17 24606.5 18 26185.0 19 26185.0 20 20036.5 21 13888.0 22 9628.5 23 5369.0 24 5222.5 25 5076.0 26 5076.0 27 6135.5 28 7195.0 29 8971.5 30 10748.0 31 14719.0 32 18690.0 33 18690.0 34 28824.5 35 38959.0 36 42596.5 37 46234.0 38 53314.0 39 60394.0 40 60394.0 41 71768.5 42 83143.0 43 90450.0 44 97757.0 45 105056.5 46 112356.0 47 112356.0 48 118327.5 49 124299.0 50 126797.5 51 129296.0 52 127605.0 53 125914.0 54 125914.0 55 114033.5 56 102153.0 57 87043.5 58 71934.0 59 64319.0 60 56704.0 61 56704.0 62 46759.0 63 36814.0 64 26678.0 65 16542.0 66 14711.5 67 12881.0 68 12881.0 69 10376.5 70 7872.0 71 5928.0 72 3984.0 73 3457.5 74 2931.0 75 2931.0 76 1768.0 77 605.0 78 419.5 79 234.0 80 183.5 81 133.0 82 133.0 83 119.0 84 105.0 85 74.0 86 43.0 87 33.5 88 24.0 89 24.0 90 14.0 91 4.0 92 3.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 1262078.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.692702266931335 #Duplication Level Percentage of deduplicated Percentage of total 1 72.82085696585978 12.1557688415389 2 10.395549614366265 3.4705962922745783 3 3.9572949694015307 1.9817384011993473 4 2.194474277964507 1.4652682301800253 5 1.4145193221693684 1.1806074947897394 6 1.044865996453513 1.0464982192583416 7 0.7979121721644576 0.9323517227571224 8 0.6150416018770666 0.8213365073528313 9 0.5199199642084963 0.7810982248649423 >10 4.655946213631354 16.278089827547113 >50 0.7132627340071465 8.404353237116547 >100 0.7386318599749485 25.286924267981405 >500 0.07854671694581684 9.091692662101538 >1k 0.05171398755438873 13.875542484808372 >5k 4.8786780711687486E-4 0.5896134404071617 >10k+ 9.757356142337497E-4 2.638520145822049 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 18810 1.4903991670879297 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 13589 1.0767163360743155 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 7240 0.573657095678714 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 4753 0.37660112924874695 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 4309 0.34142105321541144 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT 3823 0.3029131321518955 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 3570 0.2828668275653327 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3379 0.26773305611856 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC 3319 0.2629789917897309 No Hit GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG 3313 0.262503585356848 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 3272 0.2592549747321481 No Hit GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA 2527 0.2002253426491865 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 2460 0.19491663748199398 No Hit GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA 2449 0.19404505902170863 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 2206 0.1747910984899507 No Hit GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC 2174 0.17225559751457517 No Hit GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG 2086 0.16528296983229246 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC 2011 0.15934038942125606 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 2008 0.1591026862048146 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 2000 0.15846881096097074 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG 1977 0.15664641963491957 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC 1967 0.1558540755801147 No Hit TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC 1946 0.1541901530650245 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC 1934 0.15323934019925867 No Hit GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC 1922 0.15228852733349285 No Hit TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA 1895 0.15014919838551977 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1862 0.14753446300466375 No Hit GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC 1859 0.1472967597882223 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 1803 0.1428596330813151 No Hit GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC 1783 0.1412749449717054 No Hit GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA 1759 0.13937331924017374 No Hit GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA 1707 0.13525313015518853 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA 1701 0.1347777237223056 No Hit CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA 1632 0.1293105497441521 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA 1594 0.12629964233589366 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 1576 0.12487342303724493 No Hit ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA 1573 0.12463571982080346 No Hit CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT 1566 0.12408107898244007 No Hit CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 1560 0.12360567254955715 No Hit TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG 1547 0.12257562527831084 No Hit TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG 1539 0.12194175003446697 No Hit TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA 1537 0.12178328122350598 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG 1529 0.1211494059796621 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG 1522 0.12059476514129872 No Hit GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC 1486 0.11774232654400124 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC 1480 0.11726692011111833 No Hit AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC 1479 0.11718768570563784 No Hit CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG 1475 0.1168707480837159 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 1440 0.11409754389189891 No Hit GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC 1391 0.11021505802335514 No Hit ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT 1389 0.11005658921239415 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG 1387 0.10989812040143318 No Hit CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA 1387 0.10989812040143318 No Hit TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT 1384 0.10966041718499173 No Hit CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT 1377 0.10910577634662834 No Hit ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT 1377 0.10910577634662834 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 1376 0.10902654194114786 No Hit CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG 1364 0.10807572907538202 No Hit CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT 1353 0.1072041506150967 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 1348 0.10680797858769427 No Hit AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC 1347 0.10672874418221377 No Hit CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG 1335 0.10577793131644796 No Hit GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT 1326 0.10506482166712358 No Hit GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG 1322 0.10474788404520165 No Hit TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG 1307 0.10355936796299436 No Hit GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT 1305 0.10340089915203338 No Hit CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA 1302 0.10316319593559195 No Hit CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC 1293 0.10245008628626756 No Hit GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG 1265 0.10023152293281397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 7.131096493243682E-4 0.0 0.0 2 7.923440548048536E-5 0.0 0.0010300472712463096 0.0 0.0 3 7.923440548048536E-5 0.0 0.001743156920570678 0.0 0.0 4 7.923440548048536E-5 0.0 0.0030109074082584437 7.923440548048536E-5 0.0 5 7.923440548048536E-5 0.0 0.004040954679504753 7.923440548048536E-5 0.0 6 7.923440548048536E-5 0.0 0.008319612575450963 2.3770321644145606E-4 0.0 7 7.923440548048536E-5 0.0 0.018857788504355516 3.1693762192194145E-4 0.0 8 1.5846881096097073E-4 0.0 0.02186869591261396 3.1693762192194145E-4 0.0 9 1.5846881096097073E-4 0.0 0.027415104296247935 0.0012677504876877658 0.0 10 8.71578460285339E-4 0.0 0.031297590164791715 0.0014262192986487364 0.0 11 9.508128657658243E-4 0.0 0.04524284552935714 0.0016639225150901925 0.0 12 9.508128657658243E-4 0.0 0.04991767545270578 0.001743156920570678 0.0 13 9.508128657658243E-4 0.0 0.05316628607740567 0.001743156920570678 0.0 14 9.508128657658243E-4 0.0 0.05657336551306655 0.001743156920570678 0.0 15 9.508128657658243E-4 0.0 0.06259518032958343 0.001743156920570678 0.0 16 0.0010300472712463096 0.0 0.06750771346937352 0.0018223913260511633 0.0 17 0.0010300472712463096 0.0 0.06901316717350274 0.002456266569895046 0.0 18 0.001109281676726795 0.0 0.07004321444474906 0.002852438597297473 0.0 19 0.001109281676726795 0.0 0.07162790255435876 0.002931673002777958 0.0 20 0.001109281676726795 0.0 0.0730541218530075 0.003486313841141356 0.0 21 0.0011885160822072804 0.0 0.07598579485578545 0.004357892301426695 0.0 22 0.0011885160822072804 0.0 0.07701584212703178 0.005942580411036402 0.0 23 0.0011885160822072804 0.0 0.07780818618183662 0.00768573733160708 0.0 24 0.0012677504876877658 0.0 0.07828359261471954 0.009825066279580184 0.0 25 0.0012677504876877658 0.0 0.07828359261471954 0.011172051172748436 0.0 26 0.0012677504876877658 0.0 0.0784420614256805 0.01196439522755329 0.0 27 0.0012677504876877658 0.0 0.07860053023664147 0.01584688109609707 0.0 28 0.0012677504876877658 0.0 0.07883823345308293 0.03724017057582812 0.0 29 0.0012677504876877658 0.0 0.07891746785856342 0.09420970811629709 0.0 30 0.001346984893168251 0.0 0.07891746785856342 0.16742229878026557 0.0 31 0.001346984893168251 0.0 0.07891746785856342 0.3050524610998686 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGAAT 30 8.312634E-6 37.0 19 GCTCTAG 40 1.6002323E-6 32.375 1 AGCGGAA 40 1.6002323E-6 32.375 18 CCGGCCA 30 3.5996767E-4 30.833334 25 CGCCAAC 30 3.5996767E-4 30.833334 15 AGCAACG 25 0.0054965443 29.6 2 TATGCTG 40 5.940583E-5 27.750002 32 CGTAGCG 40 5.940583E-5 27.750002 15 GGTATGC 40 5.940583E-5 27.750002 30 ACAACGC 55 6.2625804E-7 26.90909 3 TGTTCGA 35 8.8698795E-4 26.42857 37 GATTGGG 35 8.8698795E-4 26.42857 2 GCCAACT 35 8.8698795E-4 26.42857 16 TTGATTC 45 1.3233123E-4 24.666668 24 CTGTACT 75 1.3740646E-8 24.666668 4 GGTATCA 3495 0.0 24.666668 1 GCCGCTC 855 0.0 24.450293 27 GCTCTCT 835 0.0 24.371258 30 CGCTCTC 835 0.0 24.371258 29 CCGCTCT 850 0.0 24.158825 28 >>END_MODULE