FastQCFastQC Report
Fri 10 Feb 2017
ERR1633449.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633449.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028615
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54180.5267276872299159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37070.3603875113623659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21950.21339373818192423No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11940.11607841612265035No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10650.10353728071241426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGT200.001841846437.08
GCTAACG250.005496077729.63
GGTATCA12200.024.2622951
GTATCAA32650.023.0612551
GTCGCCA1450.022.96551912
TCGCCAT2000.021.27500213
TCGCAAG450.003825538820.55555519
GCAACGC450.003825538820.5555553
CGTCGCA450.003825538820.55555517
GTCGCAA555.142589E-420.18181818
GGCCGCA1900.019.47368433
CGAGTCG801.6164018E-518.521
ACGTCGC500.007034390718.516
TTGGCCG1850.018.031
TACCCGA650.00157978117.07692330
TTAACGG1301.3924364E-817.07692335
ATCGGGA1554.0017767E-1016.70967921
ATTCGTG803.382134E-416.187511
TTGTCCG1151.2418077E-616.08695613
ATTACGC700.002592245115.8571433
Produced by FastQC (version 0.11.5)