##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633449.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1028615
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.912475513190067	33.0	33.0	33.0	33.0	33.0
2	32.02495297074221	33.0	33.0	33.0	33.0	33.0
3	31.747366118518592	33.0	33.0	33.0	27.0	33.0
4	32.01268696256617	33.0	33.0	33.0	33.0	33.0
5	32.11804124964151	33.0	33.0	33.0	33.0	33.0
6	35.300897809190026	37.0	37.0	37.0	33.0	37.0
7	35.42960874574063	37.0	37.0	37.0	33.0	37.0
8	35.565044258541825	37.0	37.0	37.0	33.0	37.0
9	35.661850157736374	37.0	37.0	37.0	33.0	37.0
10	35.67270650340507	37.0	37.0	37.0	33.0	37.0
11	35.68461572113959	37.0	37.0	37.0	33.0	37.0
12	35.690302008039936	37.0	37.0	37.0	33.0	37.0
13	35.685531515678846	37.0	37.0	37.0	33.0	37.0
14	35.661907516417706	37.0	37.0	37.0	33.0	37.0
15	35.68443003456104	37.0	37.0	37.0	33.0	37.0
16	35.66692688712492	37.0	37.0	37.0	33.0	37.0
17	35.66152544926916	37.0	37.0	37.0	33.0	37.0
18	35.64246972871288	37.0	37.0	37.0	33.0	37.0
19	35.63477491578482	37.0	37.0	37.0	33.0	37.0
20	35.63682718995931	37.0	37.0	37.0	33.0	37.0
21	35.63281888753323	37.0	37.0	37.0	33.0	37.0
22	35.53802929181472	37.0	37.0	37.0	33.0	37.0
23	35.586430297049915	37.0	37.0	37.0	33.0	37.0
24	35.5736363945694	37.0	37.0	37.0	33.0	37.0
25	35.597915643851195	37.0	37.0	37.0	33.0	37.0
26	35.49060046761908	37.0	37.0	37.0	33.0	37.0
27	35.50706240916183	37.0	37.0	37.0	33.0	37.0
28	35.526070492847175	37.0	37.0	37.0	33.0	37.0
29	35.53262104869169	37.0	37.0	37.0	33.0	37.0
30	35.535651336991975	37.0	37.0	37.0	33.0	37.0
31	35.53096348001925	37.0	37.0	37.0	33.0	37.0
32	35.51633215537397	37.0	37.0	37.0	33.0	37.0
33	35.51432849025146	37.0	37.0	37.0	33.0	37.0
34	35.48643953276979	37.0	37.0	37.0	33.0	37.0
35	35.42826908026813	37.0	37.0	37.0	33.0	37.0
36	35.455845967636094	37.0	37.0	37.0	33.0	37.0
37	35.45074201717844	37.0	37.0	37.0	33.0	37.0
38	35.43199058928754	37.0	37.0	37.0	33.0	37.0
39	35.330299480369234	37.0	37.0	37.0	33.0	37.0
40	35.12243550794029	37.0	37.0	37.0	33.0	37.0
41	35.28918497202549	37.0	37.0	37.0	33.0	37.0
42	35.336129650063434	37.0	37.0	37.0	33.0	37.0
43	35.00354943297541	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	27.0
16	46.0
17	39.0
18	42.0
19	26.0
20	65.0
21	163.0
22	417.0
23	1070.0
24	2097.0
25	3863.0
26	6313.0
27	9522.0
28	13438.0
29	18274.0
30	24664.0
31	31756.0
32	40024.0
33	52912.0
34	77327.0
35	158005.0
36	588523.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.69110891830277	20.478993598187856	14.077278670834081	23.752618812675298
2	15.817871604050108	22.188087865722355	39.65176475163205	22.342275778595493
3	18.083928389144628	27.38692319283697	30.646549000354845	23.88259941766356
4	12.394724945679386	18.020250531053893	39.92028115475664	29.66474336851008
5	13.61150673478415	37.31512762306597	35.20413371378018	13.869231928369702
6	28.57959489216082	40.165756867243815	17.54815941824687	13.706488822348497
7	25.842808047714644	32.96160370984285	23.22151631076739	17.974071931675116
8	24.46396367931636	35.53418917670849	20.795244090354505	19.206603053620643
9	26.26512349129655	15.517176008516307	20.228559762398955	37.98914073778819
10	14.929103697690586	28.267913650880068	33.76968058991945	23.033302061509893
11	33.198038138662184	24.331260967417354	24.2215017280518	18.249199165868667
12	22.907696271199622	26.168197041653094	30.564399702512603	20.35970698463468
13	28.386422519601602	22.722398565060786	26.387132211760473	22.504046703577142
14	22.169810862178753	21.703941708024868	28.36415957379583	27.762087856000544
15	23.736286171210804	28.812237814925894	25.202432396960962	22.249043616902338
16	22.3470394656893	28.997438302960777	25.336398944211393	23.31912328713853
17	22.254196176411973	27.617232881107117	26.58885977746776	23.53971116501315
18	23.11729850332729	26.789031853511762	28.10137903880461	21.99229060435634
19	24.98631655186829	26.075159316167856	28.231262425688914	20.70726170627494
20	24.738215950574315	25.911444029107102	28.068033229147932	21.28230679117065
21	23.07870291605703	27.183640137466398	27.581651055059474	22.1560058914171
22	22.30358297322127	27.2841636569562	27.868250025519753	22.544003344302777
23	22.446007495515815	26.79282335956602	28.377089581621888	22.38407956329628
24	22.85597623989539	27.075436387764128	27.636579283794227	22.432008088546247
25	22.93763944721786	26.940400441370194	28.08922677581019	22.032733335601755
26	22.81174200259572	26.884013941076105	27.897221020498435	22.40702303582973
27	22.742133840163714	26.947400144854978	27.76967086810906	22.54079514687225
28	22.138798287017007	27.1196706250638	28.17818134092931	22.563349746989886
29	23.14345017329127	26.93485900944474	27.54704141005138	22.37464940721261
30	22.728231651298106	27.02925778838535	28.14677989335174	22.0957306669648
31	23.127992494762374	26.691230440932713	27.84773700558518	22.333040058719735
32	21.869115266644954	26.760352512844943	28.215318656640235	23.155213563869864
33	22.12713211454237	26.758116496453972	28.73038017139552	22.38437121760814
34	22.242530003937333	26.607817307739047	28.03740952640201	23.112243161921615
35	22.515615657947823	27.437087734477917	27.76257394652032	22.28472266105394
36	22.841199088094186	26.995037015793084	27.749546720590306	22.414217175522424
37	23.247376326419506	26.548611482430257	27.38235394195107	22.821658249199167
38	23.038551839123482	26.97180188894776	27.606538889672034	22.38310738225672
39	22.716176606407647	26.697744053897715	27.87777739970737	22.708301939987262
40	22.53486484253098	27.017980488326536	28.034298547075437	22.41285612206705
41	22.49383880266183	26.54773651949466	28.25294206287095	22.70548261497256
42	22.52854566577388	26.79904531821916	27.905776213646504	22.766632802360455
43	22.459326375757694	26.529070643535242	28.03770118071387	22.973901799993197
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	72.5
2	90.0
3	302.0
4	514.0
5	514.0
6	670.5
7	827.0
8	750.5
9	674.0
10	979.5
11	1285.0
12	1285.0
13	2082.0
14	2879.0
15	5174.0
16	7469.0
17	8676.5
18	9884.0
19	9884.0
20	9508.0
21	9132.0
22	10358.0
23	11584.0
24	14718.5
25	17853.0
26	17853.0
27	21627.0
28	25401.0
29	31520.0
30	37639.0
31	41244.0
32	44849.0
33	44849.0
34	49547.0
35	54245.0
36	57637.5
37	61030.0
38	64791.0
39	68552.0
40	68552.0
41	71387.0
42	74222.0
43	73492.5
44	72763.0
45	72966.5
46	73170.0
47	73170.0
48	72227.5
49	71285.0
50	72377.0
51	73469.0
52	74032.0
53	74595.0
54	74595.0
55	68464.5
56	62334.0
57	56163.5
58	49993.0
59	44286.5
60	38580.0
61	38580.0
62	33926.0
63	29272.0
64	24061.5
65	18851.0
66	15909.0
67	12967.0
68	12967.0
69	10793.0
70	8619.0
71	7301.5
72	5984.0
73	4804.0
74	3624.0
75	3624.0
76	2740.0
77	1856.0
78	1459.0
79	1062.0
80	867.0
81	672.0
82	672.0
83	589.0
84	506.0
85	438.5
86	371.0
87	311.0
88	251.0
89	251.0
90	195.0
91	139.0
92	97.5
93	56.0
94	33.5
95	11.0
96	11.0
97	6.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1028615.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.0512866041007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.66673314583365	51.17782098035738
2	8.232288832381697	9.722544944974183
3	1.939922143370885	3.4366469543350644
4	0.804756589598264	1.9008764807562295
5	0.4365246432621542	1.2888670909513142
6	0.2777639421265403	0.9841390892879504
7	0.20238900026137616	0.8365931601966355
8	0.15775958390805647	0.7452725123118654
9	0.11671375833397528	0.620288783461919
>10	0.9290678008591192	11.183531486611832
>50	0.14468578628585954	6.0151689437129905
>100	0.08723289902141614	9.47607254174019
>500	0.00316302481542826	1.176620009320305
>1k	8.323749514284896E-4	0.9029371531127914
>5k	1.6647499028569788E-4	0.5326198688693635
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5418	0.5267276872299159	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3707	0.3603875113623659	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2195	0.21339373818192423	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1194	0.11607841612265035	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1065	0.10353728071241426	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.9443620791063712E-4	0.0	0.0
2	0.0	0.0	2.9165431186595566E-4	0.0	0.0
3	0.0	0.0	6.805267276872299E-4	9.721810395531856E-5	0.0
4	0.0	0.0	6.805267276872299E-4	1.9443620791063712E-4	0.0
5	0.0	0.0	7.777448316425485E-4	2.9165431186595566E-4	0.0
6	0.0	0.0	0.001069399143508504	2.9165431186595566E-4	0.0
7	0.0	0.0	0.003110979326570194	3.8887241582127424E-4	0.0
8	0.0	0.0	0.003499851742391468	3.8887241582127424E-4	0.0
9	0.0	0.0	0.004180378470078698	6.805267276872299E-4	0.0
10	9.721810395531856E-5	0.0	0.004958123301721246	6.805267276872299E-4	0.0
11	2.9165431186595566E-4	0.0	0.00806910262829144	6.805267276872299E-4	0.0
12	2.9165431186595566E-4	0.0	0.008652411252023352	6.805267276872299E-4	0.0
13	2.9165431186595566E-4	0.0	0.00874962935597867	6.805267276872299E-4	0.0
14	2.9165431186595566E-4	0.0	0.009138501771799945	6.805267276872299E-4	0.0
15	2.9165431186595566E-4	0.0	0.009916246603442492	6.805267276872299E-4	0.0
16	2.9165431186595566E-4	0.0	0.01069399143508504	6.805267276872299E-4	0.0
17	2.9165431186595566E-4	0.0	0.01079120953904036	8.74962935597867E-4	0.0
18	2.9165431186595566E-4	0.0	0.010985645746950998	8.74962935597867E-4	0.0
19	2.9165431186595566E-4	0.0	0.011277300058816954	9.721810395531856E-4	0.0
20	2.9165431186595566E-4	0.0	0.011374518162772271	0.0012638353514191412	0.0
21	2.9165431186595566E-4	0.0	0.01215226299441482	0.0016527077672404155	0.0
22	3.8887241582127424E-4	0.0	0.012346699202325458	0.001749925871195734	0.0
23	3.8887241582127424E-4	0.0	0.012443917306280776	0.0023332344949276453	0.0
24	3.8887241582127424E-4	0.0	0.012638353514191413	0.0025276707028382825	0.0
25	3.8887241582127424E-4	0.0	0.012832789722102049	0.0028193250147042384	0.0
26	3.8887241582127424E-4	0.0	0.012832789722102049	0.0030137612226148755	0.0
27	3.8887241582127424E-4	0.0	0.012832789722102049	0.0037915060542574237	0.0
28	3.8887241582127424E-4	0.0	0.012832789722102049	0.012055044890459502	0.0
29	3.8887241582127424E-4	0.0	0.012832789722102049	0.02488783461256155	0.0
30	3.8887241582127424E-4	0.0	0.012832789722102049	0.04831739766579332	0.0
31	3.8887241582127424E-4	0.0	0.012832789722102049	0.10305119019263767	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCGT	20	0.0018418464	37.0	8
GCTAACG	25	0.0054960777	29.6	3
GGTATCA	1220	0.0	24.262295	1
GTATCAA	3265	0.0	23.061255	1
GTCGCCA	145	0.0	22.965519	12
TCGCCAT	200	0.0	21.275002	13
TCGCAAG	45	0.0038255388	20.555555	19
GCAACGC	45	0.0038255388	20.555555	3
CGTCGCA	45	0.0038255388	20.555555	17
GTCGCAA	55	5.142589E-4	20.181818	18
GGCCGCA	190	0.0	19.473684	33
CGAGTCG	80	1.6164018E-5	18.5	21
ACGTCGC	50	0.0070343907	18.5	16
TTGGCCG	185	0.0	18.0	31
TACCCGA	65	0.001579781	17.076923	30
TTAACGG	130	1.3924364E-8	17.076923	35
ATCGGGA	155	4.0017767E-10	16.709679	21
ATTCGTG	80	3.382134E-4	16.1875	11
TTGTCCG	115	1.2418077E-6	16.086956	13
ATTACGC	70	0.0025922451	15.857143	3
>>END_MODULE