FastQCFastQC Report
Fri 10 Feb 2017
ERR1633448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188455
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81840.688625147775894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55520.46716114619400817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30910.26008557328632553No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA23980.2017745728698184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15230.12814957234392552No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15010.1262984294735602No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13590.1143501436739296No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA13400.11275142937679591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.025.9787221
GTATCAA41500.022.5566271
GACCGTT603.726424E-521.5833327
TTGCGGT1101.7553248E-920.18181835
GCTTTAT6200.019.6935481
CTTGCGG1254.129106E-1019.2434
TAGGACC2600.019.2115384
CCGTCCG1103.851892E-818.528
CGCCGTT701.21924146E-418.525
CCTTGCG1306.9667294E-1018.533
ACGCCGG500.007035020718.49999816
TGCGGGT1950.018.02564221
TCTATAC1800.017.4722233
GTCGCAA852.7232409E-517.41176418
CGCCGTC1201.0412623E-716.95833226
GTCTAGA1005.8781297E-616.651
GTATTAG5450.016.6330281
GCCGGCA1801.0913936E-1116.44444515
CGAGTCG1352.219349E-816.44444321
CGTCCGC1251.657736E-716.27999929
Produced by FastQC (version 0.11.5)