FastQCFastQC Report
Fri 10 Feb 2017
ERR1633447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963628
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59410.616524218889447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38950.4042016213725628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23830.24729459916067198No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14330.14870883785029076No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13200.1369823209786349No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12050.12504825513579929No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11830.12276521645282204No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10430.10823678847023954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.10688772015757118No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9890.1026329662483863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTA250.005495907329.629
GGTATCA10700.028.7009331
GATCGCG358.8683487E-426.4285728
TATATCG509.089095E-625.9000025
GCTACCG509.089095E-625.9000027
GTATCAA29400.022.8418371
ATTACGC450.003825366520.5555553
TTTGACG656.900653E-519.92307731
GTCAACC2800.019.16071515
ATTCGTG609.23534E-418.511
CACGTTG500.007034076418.56
TCGCGCC500.007034076418.510
TACCGTT701.2189374E-418.59
TGCGGTA1054.7952744E-717.61904736
TTGCGGT1054.7952744E-717.61904735
TGCGGGT953.6051642E-617.52631621
GTATTAG4550.017.4835171
GTCGCCA752.066829E-417.26666612
TTAACGG2700.017.1296335
GTCGGCT650.001579683917.07692323
Produced by FastQC (version 0.11.5)