##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633447.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	963628
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87018745823077	33.0	33.0	33.0	33.0	33.0
2	32.000277077876525	33.0	33.0	33.0	33.0	33.0
3	31.722382496149965	33.0	33.0	33.0	27.0	33.0
4	32.000390192065815	33.0	33.0	33.0	33.0	33.0
5	32.1007619122732	33.0	33.0	33.0	33.0	33.0
6	35.273995774302946	37.0	37.0	37.0	33.0	37.0
7	35.4091869476603	37.0	37.0	37.0	33.0	37.0
8	35.53793061222795	37.0	37.0	37.0	33.0	37.0
9	35.64229453689598	37.0	37.0	37.0	33.0	37.0
10	35.65000913215473	37.0	37.0	37.0	33.0	37.0
11	35.665299264861545	37.0	37.0	37.0	33.0	37.0
12	35.65775485975916	37.0	37.0	37.0	33.0	37.0
13	35.65727542163574	37.0	37.0	37.0	33.0	37.0
14	35.635610422279136	37.0	37.0	37.0	33.0	37.0
15	35.667192111478705	37.0	37.0	37.0	33.0	37.0
16	35.64580937872291	37.0	37.0	37.0	33.0	37.0
17	35.6406964098179	37.0	37.0	37.0	33.0	37.0
18	35.626997139975174	37.0	37.0	37.0	33.0	37.0
19	35.6145462771941	37.0	37.0	37.0	33.0	37.0
20	35.61148389212435	37.0	37.0	37.0	33.0	37.0
21	35.61722780990175	37.0	37.0	37.0	33.0	37.0
22	35.52424587081322	37.0	37.0	37.0	33.0	37.0
23	35.570622688423335	37.0	37.0	37.0	33.0	37.0
24	35.560261843782044	37.0	37.0	37.0	33.0	37.0
25	35.57794605387141	37.0	37.0	37.0	33.0	37.0
26	35.476011489911045	37.0	37.0	37.0	33.0	37.0
27	35.49141369906229	37.0	37.0	37.0	33.0	37.0
28	35.51024254172772	37.0	37.0	37.0	33.0	37.0
29	35.51783364534862	37.0	37.0	37.0	33.0	37.0
30	35.51731788615524	37.0	37.0	37.0	33.0	37.0
31	35.505633916822674	37.0	37.0	37.0	33.0	37.0
32	35.497219881531045	37.0	37.0	37.0	33.0	37.0
33	35.50254247489695	37.0	37.0	37.0	33.0	37.0
34	35.4793986891207	37.0	37.0	37.0	33.0	37.0
35	35.41916693993948	37.0	37.0	37.0	33.0	37.0
36	35.44131967937835	37.0	37.0	37.0	33.0	37.0
37	35.4372828518889	37.0	37.0	37.0	33.0	37.0
38	35.422436873980416	37.0	37.0	37.0	33.0	37.0
39	35.32895785510591	37.0	37.0	37.0	33.0	37.0
40	35.109574441589494	37.0	37.0	37.0	33.0	37.0
41	35.28308641924062	37.0	37.0	37.0	33.0	37.0
42	35.32217619247261	37.0	37.0	37.0	33.0	37.0
43	34.99854093073261	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	21.0
16	45.0
17	32.0
18	25.0
19	35.0
20	56.0
21	162.0
22	383.0
23	971.0
24	2084.0
25	3661.0
26	6139.0
27	9165.0
28	13067.0
29	17630.0
30	23084.0
31	29704.0
32	38147.0
33	49811.0
34	72630.0
35	148757.0
36	548014.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76487192152988	20.406629944335368	13.929130328300962	23.899367805833787
2	15.999950188246917	21.404732946738783	39.80944928955987	22.78586757545443
3	17.775842960146445	27.668560896943635	30.16267688361069	24.39291925929923
4	12.228889156396452	17.82254147866189	40.07915917760796	29.8694101873337
5	13.497532242732674	37.38392823786773	35.27357029891203	13.844969220487574
6	29.065676796440123	40.2591041356208	17.106186204634984	13.569032863304098
7	26.413304719248508	32.537763535306155	23.368457537555987	17.68047420788935
8	24.53664692184121	35.61343173921887	20.32859153117178	19.521329807768144
9	26.171406393338508	15.519785643422566	20.27276085792443	38.036047105314495
10	15.365057885408062	27.918657407215232	33.41953533936332	23.296749368013383
11	33.47028106281677	24.27855977617919	23.85256551283275	18.398593648171286
12	23.511562553184422	26.270407252591248	29.720182477055463	20.497847717168867
13	27.826816987468195	22.815858401789903	26.239067357943107	23.1182572527988
14	22.25454220923427	21.343090902298396	28.246481007193648	28.155885881273683
15	24.102246925161992	28.431510915000395	25.38282407734105	22.083418082496564
16	22.499865093168733	28.76680627794128	25.34287090038895	23.39045772850104
17	22.167060317882004	27.55648445250657	26.3938988904432	23.882556339168225
18	22.912887545816435	26.85984633074174	28.313519324884705	21.913746798557117
19	25.236086954716964	25.78277094480443	28.022431892805105	20.9587102076735
20	24.440240424728216	25.881668029571575	28.31933069607774	21.358760849622467
21	23.05900202152698	27.010319334847054	28.009148758649605	21.92152988497636
22	22.5453183178571	26.976073754602396	27.85929840145782	22.619309526082677
23	22.455657162307446	26.779524878895177	28.628059790707617	22.13675816808976
24	22.8691984873831	27.25636864018065	27.160792338952376	22.713640533483876
25	22.622734084107147	26.926884648432797	28.382010485374025	22.068370782086035
26	22.95802944704803	26.907271270656313	28.222509101022386	21.91219018127327
27	22.76511267833645	26.97939453814128	27.415558701075522	22.839934082446753
28	22.031842163158398	26.727948959557008	28.67548473062219	22.564724146662403
29	23.765187395966077	26.64171236203182	27.518191667323904	22.074908574678194
30	23.080275791072904	26.549145520885652	28.319123147106563	22.05145554093488
31	22.75898998368665	26.563051301954697	28.232263902667835	22.44569481169082
32	21.68284856811965	26.4615598550478	28.498030360263506	23.35756121656905
33	21.854699116256484	26.653853976845838	28.978817552001395	22.512629354896287
34	22.504742493991458	26.425031236120162	28.578974459023605	22.491251810864775
35	22.708348034718792	27.163386701092122	27.887732610509445	22.240532653679633
36	23.34915548323627	26.84127069782115	27.51507843275621	22.294495386186373
37	22.907595047051352	26.43208790114027	27.647598450854478	23.012718600953896
38	22.998190172971313	26.852478342264856	27.550673081313537	22.598658403450294
39	22.315354057789936	26.717571510998024	28.124442212139954	22.84263221907209
40	22.622422760650377	27.311887989971233	28.155470783331328	21.910218466047063
41	22.36506203638749	26.15106659416289	28.40868052817062	23.075190841279
42	22.03049309484573	27.1318392574728	28.172593573453657	22.665074074227814
43	22.795414828128695	26.028301377710072	28.042252819552772	23.134030974608457
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	82.5
2	96.0
3	327.0
4	558.0
5	558.0
6	668.5
7	779.0
8	719.0
9	659.0
10	968.5
11	1278.0
12	1278.0
13	1997.5
14	2717.0
15	5010.5
16	7304.0
17	8509.0
18	9714.0
19	9714.0
20	8985.0
21	8256.0
22	8776.0
23	9296.0
24	11579.5
25	13863.0
26	13863.0
27	17780.5
28	21698.0
29	25784.5
30	29871.0
31	34191.0
32	38511.0
33	38511.0
34	45643.0
35	52775.0
36	56708.0
37	60641.0
38	64268.0
39	67895.0
40	67895.0
41	70732.0
42	73569.0
43	74330.5
44	75092.0
45	73325.5
46	71559.0
47	71559.0
48	69048.0
49	66537.0
50	66793.5
51	67050.0
52	65306.5
53	63563.0
54	63563.0
55	61804.5
56	60046.0
57	52264.0
58	44482.0
59	39971.0
60	35460.0
61	35460.0
62	31503.5
63	27547.0
64	22436.0
65	17325.0
66	14456.5
67	11588.0
68	11588.0
69	9664.5
70	7741.0
71	6753.0
72	5765.0
73	5226.5
74	4688.0
75	4688.0
76	3762.0
77	2836.0
78	2087.0
79	1338.0
80	922.0
81	506.0
82	506.0
83	430.0
84	354.0
85	324.5
86	295.0
87	239.0
88	183.0
89	183.0
90	134.0
91	85.0
92	59.5
93	34.0
94	19.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	963628.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.047085531682335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.40516221157971	46.69947192428135
2	7.861345660760555	8.49765642642491
3	1.9589121226884376	3.176204731319742
4	0.8918496501449179	1.9280749729113336
5	0.47376128108095317	1.2802708240090837
6	0.35608628475651505	1.1547255533336618
7	0.24060080718079202	0.9102640683284454
8	0.2008175310832734	0.8682881822975164
9	0.15253444334547903	0.7419637895418592
>10	1.1852622030276845	12.957983636758552
>50	0.1554952177141466	5.901924114254474
>100	0.10845444631164987	10.864950889057221
>500	0.007968875067249363	2.9768341359708024
>1k	0.001554902452146217	1.4172999563313595
>5k	1.9436280651827714E-4	0.624086795179707
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5941	0.616524218889447	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3895	0.4042016213725628	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2383	0.24729459916067198	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1433	0.14870883785029076	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1320	0.1369823209786349	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1205	0.12504825513579929	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1183	0.12276521645282204	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1043	0.10823678847023954	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1030	0.10688772015757118	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	989	0.1026329662483863	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.0377448558987494E-4	0.0	0.0
2	0.0	0.0	1.0377448558987494E-4	0.0	0.0
3	0.0	0.0	2.0754897117974987E-4	0.0	0.0
4	0.0	0.0	2.0754897117974987E-4	2.0754897117974987E-4	0.0
5	0.0	0.0	2.0754897117974987E-4	2.0754897117974987E-4	0.0
6	0.0	0.0	6.226469135392496E-4	2.0754897117974987E-4	0.0
7	0.0	0.0	0.001556617283848124	2.0754897117974987E-4	0.0
8	0.0	0.0	0.0018679407406177488	2.0754897117974987E-4	0.0
9	1.0377448558987494E-4	0.0	0.002075489711797499	8.301958847189995E-4	0.0
10	1.0377448558987494E-4	0.0	0.002801911110926623	8.301958847189995E-4	0.0
11	1.0377448558987494E-4	0.0	0.004150979423594998	8.301958847189995E-4	0.0
12	1.0377448558987494E-4	0.0	0.004566077365954497	9.339703703088744E-4	0.0
13	1.0377448558987494E-4	0.0	0.005292498765083621	0.0011415193414886242	0.0
14	1.0377448558987494E-4	0.0	0.005603822221853246	0.0011415193414886242	0.0
15	1.0377448558987494E-4	0.0	0.006122694649802621	0.0011415193414886242	0.0
16	1.0377448558987494E-4	0.0	0.0064340181065722455	0.0011415193414886242	0.0
17	1.0377448558987494E-4	0.0	0.00653779259216212	0.0012452938270784992	0.0
18	1.0377448558987494E-4	0.0	0.006745341563341871	0.0012452938270784992	0.0
19	1.0377448558987494E-4	0.0	0.0068491160489317455	0.0012452938270784992	0.0
20	1.0377448558987494E-4	0.0	0.00695289053452162	0.0013490683126683742	0.0
21	2.0754897117974987E-4	0.0	0.00716043950570137	0.001660391769437999	0.0
22	2.0754897117974987E-4	0.0	0.0072642139912912455	0.0018679407406177488	0.0
23	2.0754897117974987E-4	0.0	0.00736798847688112	0.0026981366253367484	0.0
24	2.0754897117974987E-4	0.0	0.007471762962470995	0.003320783538875998	0.0
25	2.0754897117974987E-4	0.0	0.007471762962470995	0.003943430452415247	0.0
26	2.0754897117974987E-4	0.0	0.007471762962470995	0.004358528394774747	0.0
27	2.0754897117974987E-4	0.0	0.00757553744806087	0.005188724279493746	0.0
28	2.0754897117974987E-4	0.0	0.00757553744806087	0.011415193414886242	0.0
29	2.0754897117974987E-4	0.0	0.00757553744806087	0.027396464195726982	0.0
30	2.0754897117974987E-4	0.0	0.00757553744806087	0.04763248888575259	0.0
31	2.0754897117974987E-4	0.0	0.00757553744806087	0.1038782600754648	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGTA	25	0.0054959073	29.6	29
GGTATCA	1070	0.0	28.700933	1
GATCGCG	35	8.8683487E-4	26.428572	8
TATATCG	50	9.089095E-6	25.900002	5
GCTACCG	50	9.089095E-6	25.900002	7
GTATCAA	2940	0.0	22.841837	1
ATTACGC	45	0.0038253665	20.555555	3
TTTGACG	65	6.900653E-5	19.923077	31
GTCAACC	280	0.0	19.160715	15
ATTCGTG	60	9.23534E-4	18.5	11
CACGTTG	50	0.0070340764	18.5	6
TCGCGCC	50	0.0070340764	18.5	10
TACCGTT	70	1.2189374E-4	18.5	9
TGCGGTA	105	4.7952744E-7	17.619047	36
TTGCGGT	105	4.7952744E-7	17.619047	35
TGCGGGT	95	3.6051642E-6	17.526316	21
GTATTAG	455	0.0	17.483517	1
GTCGCCA	75	2.066829E-4	17.266666	12
TTAACGG	270	0.0	17.12963	35
GTCGGCT	65	0.0015796839	17.076923	23
>>END_MODULE