FastQCFastQC Report
Fri 10 Feb 2017
ERR1633446.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633446.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134984
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59920.5279369577016063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41360.3644104234068498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24450.21542153898204733No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA13380.11788712439999155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.026.6679251
GTATCAA35000.022.728571
AACGCAC502.702073E-422.232
TTAGAGT1600.020.81254
ACGAACG555.1430386E-420.18181829
ACAACGC500.007034828418.53
CGGCGTA500.007034828418.512
TGGACGT1103.851528E-818.526
TCTATCG500.007034828418.529
AAACACG1258.578354E-917.7615
GACGTAT953.6063047E-617.52631628
GGACGTA953.6063047E-617.52631627
ACGTATA953.6063047E-617.52631629
ATTTACG650.00157991817.07692127
CGAATTA904.445876E-516.44444515
ATACTAA2300.016.0869566
GCCGGCA2200.015.97727315
TTCGCCG1751.3096724E-1015.85714324
TCAACGC50000.015.7994
ATCAACG49800.015.7881523
Produced by FastQC (version 0.11.5)