##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633446.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1134984
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.891848695664432	33.0	33.0	33.0	33.0	33.0
2	32.00938779753724	33.0	33.0	33.0	33.0	33.0
3	31.726969719396926	33.0	33.0	33.0	27.0	33.0
4	32.00939660823413	33.0	33.0	33.0	33.0	33.0
5	32.10148953641637	33.0	33.0	33.0	33.0	33.0
6	35.27546467615402	37.0	37.0	37.0	33.0	37.0
7	35.41139434564716	37.0	37.0	37.0	33.0	37.0
8	35.541007626539226	37.0	37.0	37.0	33.0	37.0
9	35.65084089291127	37.0	37.0	37.0	33.0	37.0
10	35.6605679022788	37.0	37.0	37.0	33.0	37.0
11	35.66789575888294	37.0	37.0	37.0	33.0	37.0
12	35.66948432753237	37.0	37.0	37.0	33.0	37.0
13	35.66124720700908	37.0	37.0	37.0	33.0	37.0
14	35.641660146750965	37.0	37.0	37.0	33.0	37.0
15	35.66970635709402	37.0	37.0	37.0	33.0	37.0
16	35.655446244176126	37.0	37.0	37.0	33.0	37.0
17	35.647234674673825	37.0	37.0	37.0	33.0	37.0
18	35.624361224475415	37.0	37.0	37.0	33.0	37.0
19	35.61887744673053	37.0	37.0	37.0	33.0	37.0
20	35.61800342559895	37.0	37.0	37.0	33.0	37.0
21	35.62260349044568	37.0	37.0	37.0	33.0	37.0
22	35.536761751707516	37.0	37.0	37.0	33.0	37.0
23	35.56733310777949	37.0	37.0	37.0	33.0	37.0
24	35.5617453638113	37.0	37.0	37.0	33.0	37.0
25	35.58870433415802	37.0	37.0	37.0	33.0	37.0
26	35.47265600219915	37.0	37.0	37.0	33.0	37.0
27	35.49217345795183	37.0	37.0	37.0	33.0	37.0
28	35.51978089558972	37.0	37.0	37.0	33.0	37.0
29	35.52620389362317	37.0	37.0	37.0	33.0	37.0
30	35.52433426374292	37.0	37.0	37.0	33.0	37.0
31	35.515996701275085	37.0	37.0	37.0	33.0	37.0
32	35.50421327525322	37.0	37.0	37.0	33.0	37.0
33	35.50661771443474	37.0	37.0	37.0	33.0	37.0
34	35.482187414095705	37.0	37.0	37.0	33.0	37.0
35	35.416582083976515	37.0	37.0	37.0	33.0	37.0
36	35.440407970508836	37.0	37.0	37.0	33.0	37.0
37	35.44208464612717	37.0	37.0	37.0	33.0	37.0
38	35.43082457550062	37.0	37.0	37.0	33.0	37.0
39	35.33415272814418	37.0	37.0	37.0	33.0	37.0
40	35.11704834605598	37.0	37.0	37.0	33.0	37.0
41	35.283091215382775	37.0	37.0	37.0	33.0	37.0
42	35.32626363014809	37.0	37.0	37.0	33.0	37.0
43	34.99598584649652	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	19.0
16	50.0
17	45.0
18	25.0
19	38.0
20	70.0
21	187.0
22	494.0
23	1222.0
24	2501.0
25	4337.0
26	7159.0
27	10945.0
28	15341.0
29	20733.0
30	27029.0
31	34953.0
32	44401.0
33	58554.0
34	84548.0
35	173135.0
36	649195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5260479442882	20.621083645232	14.108657038337105	23.74421137214269
2	15.650705208179147	22.10101640199333	39.90875642299803	22.339521966829487
3	17.625182381425642	27.688055514438968	30.949951717381037	23.73681038675435
4	12.319997462519297	18.063690765684804	40.12074179019264	29.495569981603264
5	13.42239185750636	37.46925066785082	35.44913408470956	13.659223389933251
6	28.41705257519049	40.45343370479231	17.630821227435806	13.498692492581393
7	25.667586503422076	33.02275626792977	23.651875268726254	17.657781959921902
8	24.063599134437137	36.08887878595645	20.879677598979367	18.96784448062704
9	26.05323070633595	15.821015979079881	20.396322767545623	37.729430547038554
10	14.828490974322106	28.379871434310967	33.868230741578735	22.92340684978819
11	32.96874669598866	24.400696397482253	24.40853791771514	18.22201898881394
12	22.718293826168477	26.382045914303635	30.452764091828605	20.44689616769928
13	27.83422497585869	22.83679769935083	26.718526428566392	22.610450896224087
14	22.063042298393633	21.793346866563756	28.37881415068406	27.764796684358544
15	23.804124111000682	28.880495231650844	25.302383117295047	22.012997540053426
16	22.26145919237628	29.140675110838565	25.477627878454673	23.120237818330477
17	22.156435685436975	27.864269452256597	26.652798629760422	23.32649623254601
18	22.809396432020186	27.14038259570179	28.286037512423086	21.76418345985494
19	24.74228711594172	26.35675921422681	28.390620484517843	20.510333185313627
20	24.407921168932777	26.061512761413375	28.340135191333093	21.19043087832075
21	22.605781226871922	27.39298527556336	27.883652985416536	22.117580512148187
22	22.17220683287165	27.37703791419086	27.86550294982132	22.585252303116167
23	22.288508031831284	27.10099878059955	28.447097051588393	22.16339613598077
24	22.787457796761892	27.38576050411283	27.54611518752687	22.2806665115984
25	22.779880597435735	26.95941088156309	28.30797614768138	21.9527323733198
26	22.71864625404411	27.095712362465026	27.96198008077647	22.2236613027144
27	22.551507334024095	27.174920527514047	27.71616163752088	22.557410500940982
28	21.935199086506945	27.19976669274633	28.405422455294527	22.459611765452202
29	23.194688207058427	27.185845791658735	27.564529544028815	22.054936457254023
30	22.61423949588717	27.235802443030032	28.146828501547162	22.00312955953564
31	22.81811902194216	26.898000324233646	28.069382475876314	22.214498177947885
32	21.707442571877667	26.973684210526315	28.275552783122933	23.043320434473085
33	21.859691414152095	26.891480408534395	28.88736757522573	22.36146060208778
34	22.307891564991227	26.746720658617214	28.14198261825717	22.80340515813439
35	22.44410493892425	27.539507164858712	27.851405834795912	22.164982061421128
36	22.947724373207024	27.145492799898502	27.607613851825224	22.299168975069254
37	22.980852593516737	26.614648312222904	27.61827479506319	22.78622429919717
38	22.894772084892825	27.07412615508236	27.733166282520283	22.29793547750453
39	22.488246530347563	26.77799863257984	28.101805840434757	22.63194899663784
40	22.57908481529255	27.279415392639894	28.053347007534907	22.088152784532646
41	22.374764754393013	26.697821290872824	28.25581682208736	22.671597132646802
42	22.31247312737448	27.147519260183405	28.04647466396002	22.49353294848209
43	22.51732183008747	26.633679417507206	27.975724767926245	22.873273984479077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	59.5
2	63.0
3	304.0
4	545.0
5	545.0
6	695.0
7	845.0
8	793.5
9	742.0
10	1081.0
11	1420.0
12	1420.0
13	2174.0
14	2928.0
15	5580.5
16	8233.0
17	9444.5
18	10656.0
19	10656.0
20	10265.5
21	9875.0
22	11237.0
23	12599.0
24	16114.5
25	19630.0
26	19630.0
27	23944.0
28	28258.0
29	35102.0
30	41946.0
31	45729.5
32	49513.0
33	49513.0
34	55827.0
35	62141.0
36	66085.0
37	70029.0
38	74420.0
39	78811.0
40	78811.0
41	82016.0
42	85221.0
43	84021.0
44	82821.0
45	82992.5
46	83164.0
47	83164.0
48	81520.0
49	79876.0
50	81191.5
51	82507.0
52	81496.5
53	80486.0
54	80486.0
55	73811.5
56	67137.0
57	59052.5
58	50968.0
59	45059.0
60	39150.0
61	39150.0
62	34201.5
63	29253.0
64	24025.0
65	18797.0
66	15986.5
67	13176.0
68	13176.0
69	10874.0
70	8572.0
71	7516.5
72	6461.0
73	5182.0
74	3903.0
75	3903.0
76	2953.0
77	2003.0
78	1565.5
79	1128.0
80	901.5
81	675.0
82	675.0
83	584.5
84	494.0
85	450.0
86	406.0
87	339.0
88	272.0
89	272.0
90	218.5
91	165.0
92	108.0
93	51.0
94	28.5
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1134984.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.22395016208586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.4166890726038	45.46213602210649
2	8.409305742193126	8.95152939440459
3	2.180077981018949	3.4809708543363995
4	0.9804232594833184	2.0872799480195967
5	0.5462275189113814	1.453619312184959
6	0.3809781202076252	1.2166296289665484
7	0.26698494537578554	0.9946995398695618
8	0.21154430538889982	0.9007378853673501
9	0.15198586715859427	0.7280359397091382
>10	1.1469410149012331	12.31355770200316
>50	0.17553316583573378	6.569737055907128
>100	0.12540796909198018	12.033799456040272
>500	0.007060501890278371	2.4726837287868784
>1k	6.72428751455083E-4	0.7984596032600269
>5k	1.6810718786377076E-4	0.5361239290378844
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5992	0.5279369577016063	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4136	0.3644104234068498	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2445	0.21542153898204733	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1338	0.11788712439999155	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.7621393781762563E-4	0.0	0.0
2	0.0	0.0	1.7621393781762563E-4	0.0	0.0
3	0.0	0.0	1.7621393781762563E-4	0.0	0.0
4	0.0	0.0	1.7621393781762563E-4	8.810696890881281E-5	0.0
5	0.0	0.0	1.7621393781762563E-4	8.810696890881281E-5	0.0
6	0.0	0.0	8.810696890881281E-4	1.7621393781762563E-4	0.0
7	0.0	0.0	0.002378888160537946	2.643209067264384E-4	0.0
8	0.0	0.0	0.0025551020983555716	2.643209067264384E-4	0.0
9	0.0	0.0	0.003348064818534887	7.929627201793153E-4	0.0
10	8.810696890881281E-5	0.0	0.0038767066319877635	7.929627201793153E-4	0.0
11	8.810696890881281E-5	0.0	0.0052864181345287685	8.810696890881281E-4	0.0
12	8.810696890881281E-5	0.0	0.006167487823616897	8.810696890881281E-4	0.0
13	8.810696890881281E-5	0.0	0.006784236605978587	8.810696890881281E-4	0.0
14	8.810696890881281E-5	0.0	0.007400985388340276	9.691766579969409E-4	0.0
15	8.810696890881281E-5	0.0	0.008370162046337217	9.691766579969409E-4	0.0
16	8.810696890881281E-5	0.0	0.009427445673242971	0.0010572836269057537	0.0
17	8.810696890881281E-5	0.0	0.009691766579969409	0.0012334975647233795	0.0
18	8.810696890881281E-5	0.0	0.009691766579969409	0.001409711502541005	0.0
19	8.810696890881281E-5	0.0	0.009779873548878222	0.0014978184714498177	0.0
20	8.810696890881281E-5	0.0	0.010132301424513473	0.0015859254403586306	0.0
21	8.810696890881281E-5	0.0	0.010396622331239912	0.0017621393781762562	0.0
22	8.810696890881281E-5	0.0	0.010484729300148725	0.0021145672538115073	0.0
23	8.810696890881281E-5	0.0	0.010484729300148725	0.002378888160537946	0.0
24	8.810696890881281E-5	0.0	0.01066094323796635	0.0029075299739908227	0.0
25	1.7621393781762563E-4	0.0	0.01066094323796635	0.0030837439118084485	0.0
26	1.7621393781762563E-4	0.0	0.01066094323796635	0.0035242787563525124	0.0
27	1.7621393781762563E-4	0.0	0.01066094323796635	0.004581562383258266	0.0
28	1.7621393781762563E-4	0.0	0.01066094323796635	0.012158761709416169	0.0
29	2.643209067264384E-4	0.0	0.010749050206875162	0.02704883945500553	0.0
30	2.643209067264384E-4	0.0	0.010749050206875162	0.04872315380657349	0.0
31	2.643209067264384E-4	0.0	0.010749050206875162	0.10616889753511943	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1325	0.0	26.667925	1
GTATCAA	3500	0.0	22.72857	1
AACGCAC	50	2.702073E-4	22.2	32
TTAGAGT	160	0.0	20.8125	4
ACGAACG	55	5.1430386E-4	20.181818	29
ACAACGC	50	0.0070348284	18.5	3
CGGCGTA	50	0.0070348284	18.5	12
TGGACGT	110	3.851528E-8	18.5	26
TCTATCG	50	0.0070348284	18.5	29
AAACACG	125	8.578354E-9	17.76	15
GACGTAT	95	3.6063047E-6	17.526316	28
GGACGTA	95	3.6063047E-6	17.526316	27
ACGTATA	95	3.6063047E-6	17.526316	29
ATTTACG	65	0.001579918	17.076921	27
CGAATTA	90	4.445876E-5	16.444445	15
ATACTAA	230	0.0	16.086956	6
GCCGGCA	220	0.0	15.977273	15
TTCGCCG	175	1.3096724E-10	15.857143	24
TCAACGC	5000	0.0	15.799	4
ATCAACG	4980	0.0	15.788152	3
>>END_MODULE