FastQCFastQC Report
Fri 10 Feb 2017
ERR1633445.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633445.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246562
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT84710.6795490316566685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59560.47779412496129353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32640.26184016519033954No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15830.12698927129176085No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15800.1267486093752256No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.11832544229649228No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT14040.11262977693849163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.028.6019741
GCAACGC358.869818E-426.428573
GTATCAA43250.023.611561
GCCGCTC3450.023.59420427
TCTCGCG551.902826E-523.54545420
TTCGCCG3550.023.45070524
GCCGGCA3800.023.36842215
GCTTCGC3750.022.69333322
CCGGCAG3850.022.10389716
AGCTTCG3850.022.10389721
CGCTCTC3850.022.10389729
CCGCTCT4050.020.09876428
TGCTCGC4500.019.73333410
CCGATCA1052.2599124E-819.3809528
CGGCAGC4700.018.89361817
ATCTCGT801.6168491E-518.537
CCGGTCG609.2374126E-418.59
CCGTCCG2900.017.86206828
GCCGTCC2900.017.86206827
CGCCGCT5100.017.7745126
Produced by FastQC (version 0.11.5)