##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633445.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1246562
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.895847137968268	33.0	33.0	33.0	33.0	33.0
2	31.99985961388202	33.0	33.0	33.0	33.0	33.0
3	31.720909188632415	33.0	33.0	33.0	27.0	33.0
4	32.00375432589795	33.0	33.0	33.0	33.0	33.0
5	32.09993325642848	33.0	33.0	33.0	33.0	33.0
6	35.26420426741711	37.0	37.0	37.0	33.0	37.0
7	35.409708462154306	37.0	37.0	37.0	33.0	37.0
8	35.53712210062556	37.0	37.0	37.0	33.0	37.0
9	35.65031021321041	37.0	37.0	37.0	33.0	37.0
10	35.64910690362774	37.0	37.0	37.0	33.0	37.0
11	35.66798442436076	37.0	37.0	37.0	33.0	37.0
12	35.65937273878074	37.0	37.0	37.0	33.0	37.0
13	35.653146012793584	37.0	37.0	37.0	33.0	37.0
14	35.63043234111099	37.0	37.0	37.0	33.0	37.0
15	35.667648299884	37.0	37.0	37.0	33.0	37.0
16	35.65226759679823	37.0	37.0	37.0	33.0	37.0
17	35.64006042218518	37.0	37.0	37.0	33.0	37.0
18	35.62669325713442	37.0	37.0	37.0	33.0	37.0
19	35.61038841228916	37.0	37.0	37.0	33.0	37.0
20	35.61200646257466	37.0	37.0	37.0	33.0	37.0
21	35.60551581068571	37.0	37.0	37.0	33.0	37.0
22	35.51862562792705	37.0	37.0	37.0	33.0	37.0
23	35.56878037353938	37.0	37.0	37.0	33.0	37.0
24	35.561425745370066	37.0	37.0	37.0	33.0	37.0
25	35.58551921204079	37.0	37.0	37.0	33.0	37.0
26	35.4797386732469	37.0	37.0	37.0	33.0	37.0
27	35.486724286477525	37.0	37.0	37.0	33.0	37.0
28	35.501805766580404	37.0	37.0	37.0	33.0	37.0
29	35.50570448962827	37.0	37.0	37.0	33.0	37.0
30	35.50730328696046	37.0	37.0	37.0	33.0	37.0
31	35.50196781227087	37.0	37.0	37.0	33.0	37.0
32	35.49737197187144	37.0	37.0	37.0	33.0	37.0
33	35.487915562964375	37.0	37.0	37.0	33.0	37.0
34	35.463506026976596	37.0	37.0	37.0	33.0	37.0
35	35.401792289513075	37.0	37.0	37.0	33.0	37.0
36	35.42215148544557	37.0	37.0	37.0	33.0	37.0
37	35.416767878372674	37.0	37.0	37.0	33.0	37.0
38	35.395430792852665	37.0	37.0	37.0	33.0	37.0
39	35.292993850285825	37.0	37.0	37.0	33.0	37.0
40	35.09250161644587	37.0	37.0	37.0	33.0	37.0
41	35.24617147001112	37.0	37.0	37.0	33.0	37.0
42	35.27416285752333	37.0	37.0	37.0	33.0	37.0
43	34.93782018062479	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	11.0
15	38.0
16	70.0
17	60.0
18	40.0
19	31.0
20	60.0
21	201.0
22	520.0
23	1270.0
24	2702.0
25	4816.0
26	7854.0
27	11905.0
28	16980.0
29	23002.0
30	30190.0
31	38645.0
32	49833.0
33	65086.0
34	94394.0
35	191588.0
36	707266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.13396525804573	20.123427474927038	14.1040718391865	23.638535427840736
2	15.813894535530522	22.252884333069677	39.43341767196497	22.49980345943483
3	17.95835265313719	27.754656406981766	30.58002730710546	23.706963632775587
4	11.9609774724402	18.047397562255227	40.79492235444366	29.196702610860914
5	13.629085436584784	37.076134199502306	35.07831940970445	14.216460954208454
6	28.07080594467022	40.89616080066615	17.41493804560062	13.618095209063007
7	25.669802224036992	33.04199871326095	23.32487272995647	17.963326332745584
8	24.61417883747459	34.90215488680066	20.879025672208844	19.60464060351591
9	26.588168097535466	15.113407917135287	20.30649097277151	37.99193301255774
10	15.0838867220403	28.023876870945852	33.59046722104476	23.30176918596909
11	33.498133265734076	24.359959632974533	24.084883062374757	18.057024038916637
12	23.15905666946369	26.257097521021816	30.334552152239517	20.24929365727497
13	28.749873652493818	22.66072606095806	26.08229674897839	22.50710353756973
14	22.281523101137367	21.55632852597785	28.655373739934316	27.506774632950464
15	23.907114126694058	28.617349157121748	25.336886572829908	22.138650143354283
16	21.826511637608075	28.755970421046044	25.835939167085154	23.581578774260727
17	22.075115397388977	27.27076551346824	26.655794096081863	23.998324993060915
18	23.636289249953073	26.20086285319142	28.263576139814944	21.899271757040566
19	25.32004023867244	25.915357599541778	28.227958176167732	20.536643985618046
20	25.110905033203323	25.651271256463776	27.845867273348617	21.391956436984284
21	23.257326952048913	26.887872404260676	27.979274195747983	21.87552644794242
22	22.67749217447668	26.63630048084251	28.44270882635601	22.2434985183248
23	22.64821164129823	26.35167765422017	28.694441191051865	22.305669513429738
24	22.420304806339356	26.66806785382516	28.246970467574016	22.664656872261467
25	22.77889106197686	26.890439464703718	28.546434112382695	21.78423536093672
26	22.810096890487596	26.82530030596152	28.155117836096398	22.209484967454486
27	22.79702092635585	26.77002828579726	28.121344947142624	22.311605840704274
28	22.331661000415544	26.78254270545709	28.274726808614414	22.611069485512957
29	22.99933737752314	26.777408584571006	27.87667199866513	22.34658203924073
30	23.21697597070984	26.584397727509746	28.255874958485823	21.942751343294596
31	22.998134067940462	26.592660453310785	28.02419775350123	22.385007725247522
32	21.846647017958194	26.52383114518171	28.640131818553748	22.98939001830635
33	21.943874432238427	26.657318288219923	28.99213998180596	22.40666729773569
34	22.426963119363496	26.40550570288522	28.367542087758167	22.799989089993115
35	22.409073916901047	26.911938595914204	28.188810504411332	22.49017698277342
36	22.825659694423543	26.70464846513852	28.205576617929957	22.264115222507986
37	23.053887411937794	26.312690423741458	28.08845448521614	22.54496767910461
38	22.785870257556383	26.7457214322272	27.776636862025317	22.691771448191105
39	22.50124743093404	26.440642342699363	28.267346509840667	22.790763716525934
40	22.503654050099392	26.77604483371064	28.50022702440793	22.22007409178204
41	22.663373342039947	26.157463487576234	28.299675427295234	22.879487743088593
42	22.042385376740185	26.980206359571362	28.355829874486787	22.62157838920166
43	22.60200455332346	26.42909057070567	28.194666611047026	22.774238264923845
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	60.0
1	87.5
2	115.0
3	486.0
4	857.0
5	857.0
6	1021.5
7	1186.0
8	1108.5
9	1031.0
10	1415.0
11	1799.0
12	1799.0
13	2959.5
14	4120.0
15	7407.5
16	10695.0
17	12201.0
18	13707.0
19	13707.0
20	12556.5
21	11406.0
22	12461.5
23	13517.0
24	17313.5
25	21110.0
26	21110.0
27	25684.0
28	30258.0
29	36042.5
30	41827.0
31	46908.0
32	51989.0
33	51989.0
34	58929.0
35	65869.0
36	70008.5
37	74148.0
38	77218.5
39	80289.0
40	80289.0
41	83825.0
42	87361.0
43	88433.0
44	89505.0
45	90814.5
46	92124.0
47	92124.0
48	91806.5
49	91489.0
50	90835.5
51	90182.0
52	87852.0
53	85522.0
54	85522.0
55	80120.5
56	74719.0
57	66631.0
58	58543.0
59	54174.5
60	49806.0
61	49806.0
62	43321.0
63	36836.0
64	29874.0
65	22912.0
66	19645.0
67	16378.0
68	16378.0
69	13650.5
70	10923.0
71	9173.0
72	7423.0
73	5811.0
74	4199.0
75	4199.0
76	3084.5
77	1970.0
78	1480.0
79	990.0
80	796.0
81	602.0
82	602.0
83	506.5
84	411.0
85	362.0
86	313.0
87	258.5
88	204.0
89	204.0
90	164.5
91	125.0
92	81.5
93	38.0
94	21.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1246562.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.85461034350797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.90178740129976	50.557162253143595
2	8.80107528593039	10.359677131146222
3	2.0294610285966996	3.58329414136181
4	0.8346400883907581	1.9648966871723648
5	0.4812130583208953	1.4160803519842036
6	0.3194837743688425	1.1281855830930867
7	0.21467303056439296	0.8844148295589129
8	0.15908186302989782	0.7490160849075155
9	0.13035772867511003	0.6904937993794503
>10	0.9239169933968273	10.745890225297416
>50	0.12043776743134114	4.977505144527212
>100	0.07737778037088751	8.7476544324419
>500	0.004974280563343986	1.9028544158767398
>1k	0.0012435701408359966	1.119641847847812
>5k	2.76348920185777E-4	1.1732330722617943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8471	0.6795490316566685	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5956	0.47779412496129353	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3264	0.26184016519033954	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1583	0.12698927129176085	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1580	0.1267486093752256	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1475	0.11832544229649228	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1404	0.11262977693849163	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.2088255538031805E-4	0.0	0.0
2	8.022063884507951E-5	0.0	3.2088255538031805E-4	0.0	0.0
3	8.022063884507951E-5	0.0	7.219857496057156E-4	0.0	0.0
4	8.022063884507951E-5	0.0	0.0013637508603663517	8.022063884507951E-5	0.0
5	8.022063884507951E-5	0.0	0.0017648540545917493	8.022063884507951E-5	0.0
6	8.022063884507951E-5	0.0	0.0029681636372679418	8.022063884507951E-5	0.0
7	8.022063884507951E-5	0.0	0.006658313024141599	1.6044127769015903E-4	0.0
8	8.022063884507951E-5	0.0	0.007460519412592394	1.6044127769015903E-4	0.0
9	8.022063884507951E-5	0.0	0.008984711550648904	9.626476661409541E-4	0.0
10	4.011031942253975E-4	0.0	0.010268241772170178	9.626476661409541E-4	0.0
11	5.615444719155565E-4	0.0	0.01628478968555114	0.001123088943831113	0.0
12	5.615444719155565E-4	0.0	0.017568319907072413	0.001123088943831113	0.0
13	5.615444719155565E-4	0.0	0.01925295332281908	0.001123088943831113	0.0
14	6.417651107606361E-4	0.0	0.020215600988960036	0.0012033095826761925	0.0
15	6.417651107606361E-4	0.0	0.022622220154312422	0.0013637508603663517	0.0
16	7.219857496057156E-4	0.0	0.023665088459298454	0.0014439714992114311	0.0
17	7.219857496057156E-4	0.0	0.02430685357005909	0.0015241921380565106	0.0
18	7.219857496057156E-4	0.0	0.024948618680819726	0.0018450746934368287	0.0
19	7.219857496057156E-4	0.0	0.025109059958509886	0.0019252953322819082	0.0
20	8.02206388450795E-4	0.0	0.02551016315273528	0.002085736609972067	0.0
21	9.626476661409541E-4	0.0	0.026392590180031157	0.0025670604430425444	0.0
22	9.626476661409541E-4	0.0	0.026793693374256557	0.0028879429984228623	0.0
23	9.626476661409541E-4	0.0	0.027275017207327033	0.0032890461926482597	0.0
24	0.0010428683049860336	0.0	0.027435458485017192	0.004492355775324452	0.0
25	0.0010428683049860336	0.0	0.02759589976270735	0.00489345896954985	0.0
26	0.0010428683049860336	0.0	0.02767612040155243	0.006016547913380963	0.0
27	0.0010428683049860336	0.0	0.02767612040155243	0.006818754301831758	0.0
28	0.0012033095826761925	0.0	0.02775634104039751	0.01572324521363558	0.0
29	0.0012033095826761925	0.0	0.02775634104039751	0.0364201700356661	0.0
30	0.0012835302215212722	0.0	0.02775634104039751	0.06313364277107757	0.0
31	0.0012835302215212722	8.022063884507951E-5	0.02775634104039751	0.13212339217784594	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1520	0.0	28.601974	1
GCAACGC	35	8.869818E-4	26.42857	3
GTATCAA	4325	0.0	23.61156	1
GCCGCTC	345	0.0	23.594204	27
TCTCGCG	55	1.902826E-5	23.545454	20
TTCGCCG	355	0.0	23.450705	24
GCCGGCA	380	0.0	23.368422	15
GCTTCGC	375	0.0	22.693333	22
CCGGCAG	385	0.0	22.103897	16
AGCTTCG	385	0.0	22.103897	21
CGCTCTC	385	0.0	22.103897	29
CCGCTCT	405	0.0	20.098764	28
TGCTCGC	450	0.0	19.733334	10
CCGATCA	105	2.2599124E-8	19.38095	28
CGGCAGC	470	0.0	18.893618	17
ATCTCGT	80	1.6168491E-5	18.5	37
CCGGTCG	60	9.2374126E-4	18.5	9
CCGTCCG	290	0.0	17.862068	28
GCCGTCC	290	0.0	17.862068	27
CGCCGCT	510	0.0	17.77451	26
>>END_MODULE