FastQCFastQC Report
Fri 10 Feb 2017
ERR1633444.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633444.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259227
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39390.3128109546571031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.2094141882281749No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC16360.12992097532851504No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT15610.12396494039597308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15430.12253549201216303No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC15010.11920011244993953No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA13550.10760569778125786No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13050.1036350078262299No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT12900.10244380083972152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.028.9405961
GTATCAA27550.021.8911081
GTCGCCA2500.019.98000112
TTCGCCG851.2455021E-619.58823624
TCGCCAT2800.018.513
TACCGTA500.007035246618.57
TAACCGT1301.3933459E-817.0769237
CGGCCTT3300.016.25757624
GTACTAA1151.2423498E-616.0869561
ATCTCGC3350.016.01492511
CTAACCG1302.5893314E-715.6538466
TCTCGCC3550.015.63380312
AGAGGTC3550.015.6338038
CGAACTA1802.0190782E-1015.41666729
TTACGTT750.004104694414.8000014
AACCTCG750.004104694414.8000015
TTAGCCG2750.014.829
TATTGTC4050.014.6172845
TATCAAC41150.014.6111792
GCGAACT1904.5474735E-1014.60526428
Produced by FastQC (version 0.11.5)