##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633444.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1259227
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.873038776963963	33.0	33.0	33.0	33.0	33.0
2	31.996211961782905	33.0	33.0	33.0	33.0	33.0
3	31.722046144182105	33.0	33.0	33.0	27.0	33.0
4	31.99259307495789	33.0	33.0	33.0	33.0	33.0
5	32.09753126322736	33.0	33.0	33.0	33.0	33.0
6	35.27075261251546	37.0	37.0	37.0	33.0	37.0
7	35.409053331925065	37.0	37.0	37.0	33.0	37.0
8	35.541373398124406	37.0	37.0	37.0	33.0	37.0
9	35.64412135381468	37.0	37.0	37.0	33.0	37.0
10	35.66105237578292	37.0	37.0	37.0	33.0	37.0
11	35.6774235304675	37.0	37.0	37.0	33.0	37.0
12	35.675143560295325	37.0	37.0	37.0	33.0	37.0
13	35.666380247564575	37.0	37.0	37.0	33.0	37.0
14	35.63960350278385	37.0	37.0	37.0	33.0	37.0
15	35.67518167891889	37.0	37.0	37.0	33.0	37.0
16	35.65267183756384	37.0	37.0	37.0	33.0	37.0
17	35.64533082597498	37.0	37.0	37.0	33.0	37.0
18	35.6275476939424	37.0	37.0	37.0	33.0	37.0
19	35.61962060851618	37.0	37.0	37.0	33.0	37.0
20	35.62308622670893	37.0	37.0	37.0	33.0	37.0
21	35.62067045894029	37.0	37.0	37.0	33.0	37.0
22	35.531043251137405	37.0	37.0	37.0	33.0	37.0
23	35.58248909847073	37.0	37.0	37.0	33.0	37.0
24	35.567044702821654	37.0	37.0	37.0	33.0	37.0
25	35.59115234981461	37.0	37.0	37.0	33.0	37.0
26	35.47935995654477	37.0	37.0	37.0	33.0	37.0
27	35.49769104379115	37.0	37.0	37.0	33.0	37.0
28	35.52066148518099	37.0	37.0	37.0	33.0	37.0
29	35.53142126082112	37.0	37.0	37.0	33.0	37.0
30	35.53011331554994	37.0	37.0	37.0	33.0	37.0
31	35.52709956187407	37.0	37.0	37.0	33.0	37.0
32	35.526276834915386	37.0	37.0	37.0	33.0	37.0
33	35.52190669355088	37.0	37.0	37.0	33.0	37.0
34	35.50310388833785	37.0	37.0	37.0	33.0	37.0
35	35.45338211458299	37.0	37.0	37.0	33.0	37.0
36	35.47922257067233	37.0	37.0	37.0	33.0	37.0
37	35.48644843225249	37.0	37.0	37.0	33.0	37.0
38	35.478741323049775	37.0	37.0	37.0	33.0	37.0
39	35.38021182836772	37.0	37.0	37.0	33.0	37.0
40	35.17833639208816	37.0	37.0	37.0	33.0	37.0
41	35.354011627768465	37.0	37.0	37.0	33.0	37.0
42	35.40638026344734	37.0	37.0	37.0	33.0	37.0
43	35.07576791158385	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	32.0
16	58.0
17	56.0
18	29.0
19	40.0
20	68.0
21	167.0
22	505.0
23	1215.0
24	2619.0
25	4954.0
26	7764.0
27	11585.0
28	16979.0
29	22785.0
30	29739.0
31	38238.0
32	48865.0
33	64892.0
34	94062.0
35	192769.0
36	721802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.356880054191976	20.227885837898967	14.003591091995327	24.411643015913732
2	15.731635360423498	21.896369757001715	39.4575402211039	22.914454661470888
3	17.94148314799476	26.970832105728356	30.781423841769595	24.30626090450729
4	12.763544619040093	17.985875461691975	39.0740509852473	30.176528934020634
5	13.63900234032466	37.429470619673815	35.32389315032158	13.607633889679938
6	29.205854067614496	39.41656270076801	17.76558158298702	13.61200164863047
7	26.087591832131935	32.60436760012293	23.513552361885505	17.794488205859626
8	24.180707688129306	36.083406724919335	20.814118502859294	18.921767084092068
9	25.931702544497536	15.994097966450846	20.012912683733752	38.06128680531787
10	14.89135795214048	28.40409235189525	33.79128624147989	22.91326345448438
11	33.17320864308024	24.0946231299043	24.400763325436955	18.33140490157851
12	22.645877192912796	25.806784638512358	30.35060398164906	21.196734186925788
13	27.912520935462787	22.404062174651592	26.64055011526913	23.042866774616492
14	22.158435294033563	21.361676647657653	27.622660568745744	28.85722748956304
15	23.915306771535235	28.529804395871434	24.744704489341476	22.81018434325185
16	22.9021455226103	28.759707344267554	24.885743396544072	23.452403736578077
17	22.56821049739245	27.710651058149168	26.183761942842715	23.53737650161567
18	23.029048773572995	27.266886748775242	27.467962488097857	22.23610198955391
19	25.14248820903618	26.43058003044725	27.499013283546176	20.927918476970394
20	24.789335044435994	25.92789068214071	27.594309842466846	21.688464430956454
21	22.975603286778316	27.323429373734836	26.80525433460369	22.895713004883156
22	22.532156632600795	27.211614744601253	26.994973900654927	23.26125472214303
23	22.617923535629398	27.179531569764627	27.511401836205863	22.691143058400115
24	23.42222649291986	27.21042353761474	26.622126113877798	22.745223855587597
25	23.435568011168755	26.77666536692749	27.316520373213088	22.471246248690665
26	23.324229864829775	26.833049164288887	26.948437414382	22.894283556499346
27	23.080588329189254	26.80549257600099	26.8065249553893	23.307394139420452
28	22.401838588276775	27.149433739905515	27.46923310888347	22.979494562934242
29	23.416746940781923	27.081534941674533	26.781986091467225	22.719732026076315
30	22.81558448159069	27.426587898766464	27.092891114945918	22.66493650469693
31	23.455580288542098	26.80104540325136	27.012683177854353	22.73069113035219
32	22.337433997206222	26.89054475483769	27.33978861634955	23.432232631606535
33	22.57972549826203	26.71718443140117	27.664114571876237	23.038975498460562
34	22.697019679533557	26.821692991017503	27.111076874939943	23.370210454508996
35	23.049140464745435	27.254895265111056	26.996721004235134	22.69924326590837
36	23.190814682340832	27.08320263145565	26.899756755533353	22.826225930670166
37	23.757829207918828	26.280408536348094	26.798821816876544	23.162940438856534
38	23.46193339247014	26.864258787335405	26.971705657518463	22.702102162675992
39	23.052872913303162	26.69883984380894	27.17841977657722	23.069867466310683
40	23.08527374333619	26.684148290975333	27.254498196115552	22.97607976957292
41	22.77008037470607	26.616011251347054	27.593833359672242	23.020075014274628
42	22.994106701968747	26.68073349761401	27.05207242220823	23.273087378209013
43	22.815505067791587	26.492046310951086	27.233453539353906	23.45899508190342
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	49.0
2	69.0
3	214.0
4	359.0
5	359.0
6	454.5
7	550.0
8	512.5
9	475.0
10	681.5
11	888.0
12	888.0
13	1367.0
14	1846.0
15	3706.0
16	5566.0
17	6531.0
18	7496.0
19	7496.0
20	7527.0
21	7558.0
22	9324.0
23	11090.0
24	14453.5
25	17817.0
26	17817.0
27	22632.5
28	27448.0
29	35800.0
30	44152.0
31	48254.0
32	52356.0
33	52356.0
34	59071.5
35	65787.0
36	70125.5
37	74464.0
38	81853.5
39	89243.0
40	89243.0
41	93526.0
42	97809.0
43	94176.0
44	90543.0
45	92634.0
46	94725.0
47	94725.0
48	93627.5
49	92530.0
50	94375.0
51	96220.0
52	96799.0
53	97378.0
54	97378.0
55	85860.0
56	74342.0
57	66732.5
58	59123.0
59	52220.0
60	45317.0
61	45317.0
62	40229.5
63	35142.0
64	29192.0
65	23242.0
66	19715.5
67	16189.0
68	16189.0
69	13483.5
70	10778.0
71	9329.5
72	7881.0
73	6337.0
74	4793.0
75	4793.0
76	3643.0
77	2493.0
78	1964.5
79	1436.0
80	1119.0
81	802.0
82	802.0
83	656.5
84	511.0
85	455.5
86	400.0
87	315.0
88	230.0
89	230.0
90	168.5
91	107.0
92	70.5
93	34.0
94	21.5
95	9.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1259227.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.821450199653306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.62746003979362	45.547726260549034
2	9.041794019499202	9.732849330719986
3	2.4203650635383505	3.9080267319663013
4	1.0253601994659274	2.2074549164904798
5	0.6006304876103982	1.6163401938658264
6	0.3860786886184598	1.2467588947575534
7	0.26435057542597157	0.9959411931377035
8	0.1991681570103128	0.8575615235109824
9	0.1455295212766262	0.704934889377239
>10	1.011654129242766	10.79139311364852
>50	0.14720476135030883	5.5639381249311
>100	0.12203936117808556	12.753824016750542
>500	0.005974997135663929	2.1139259213636925
>1k	0.0023899988542655714	1.9593248889311081
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3939	0.3128109546571031	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2637	0.2094141882281749	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1636	0.12992097532851504	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1561	0.12396494039597308	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1543	0.12253549201216303	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1501	0.11920011244993953	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1355	0.10760569778125786	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1305	0.1036350078262299	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1290	0.10244380083972152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.941379910055932E-5	7.941379910055932E-5	0.0
2	0.0	0.0	2.3824139730167792E-4	7.941379910055932E-5	0.0
3	0.0	0.0	3.9706899550279656E-4	7.941379910055932E-5	0.0
4	0.0	0.0	5.558965937039152E-4	7.941379910055932E-5	0.0
5	0.0	0.0	7.941379910055931E-4	7.941379910055932E-5	0.0
6	0.0	0.0	0.0015088621829106269	7.941379910055932E-5	0.0
7	7.941379910055932E-5	0.0	0.0031765519640223725	7.941379910055932E-5	0.0
8	7.941379910055932E-5	0.0	0.00349420716042461	7.941379910055932E-5	0.0
9	7.941379910055932E-5	0.0	0.004526586548731881	3.9706899550279656E-4	0.0
10	3.1765519640223727E-4	0.0	0.005161896941536355	3.9706899550279656E-4	0.0
11	3.1765519640223727E-4	0.0	0.007703138512754253	5.558965937039152E-4	0.0
12	3.1765519640223727E-4	0.0	0.008417862704659287	5.558965937039152E-4	0.0
13	3.1765519640223727E-4	0.0	0.008735517901061524	5.558965937039152E-4	0.0
14	3.1765519640223727E-4	0.0	0.009450242092966557	5.558965937039152E-4	0.0
15	3.1765519640223727E-4	0.0	0.010085552485771032	5.558965937039152E-4	0.0
16	3.1765519640223727E-4	0.0	0.010641449079474948	6.353103928044745E-4	0.0
17	3.9706899550279656E-4	0.0	0.010959104275877185	7.147241919050338E-4	0.0
18	3.9706899550279656E-4	0.0	0.011038518074977745	7.147241919050338E-4	0.0
19	3.9706899550279656E-4	0.0	0.011117931874078304	7.147241919050338E-4	0.0
20	3.9706899550279656E-4	0.0	0.011117931874078304	7.941379910055931E-4	0.0
21	3.9706899550279656E-4	0.0	0.0115150008695811	9.529655892067117E-4	0.0
22	3.9706899550279656E-4	0.0	0.01159441466868166	0.0013500345847095082	0.0
23	3.9706899550279656E-4	0.0	0.011753242266882779	0.0015882759820111862	0.0
24	3.9706899550279656E-4	0.0	0.011753242266882779	0.0018265173793128643	0.0
25	3.9706899550279656E-4	0.0	0.011753242266882779	0.002144172575715101	0.0
26	3.9706899550279656E-4	0.0	0.011753242266882779	0.0026206553703184573	0.0
27	4.7648279460335585E-4	0.0	0.011753242266882779	0.0036530347586257285	0.0
28	5.558965937039152E-4	0.0	0.011753242266882779	0.009847311088469354	0.0
29	5.558965937039152E-4	0.0	0.011753242266882779	0.022712346542759963	0.0
30	5.558965937039152E-4	0.0	0.011753242266882779	0.040580451340385806	0.0
31	5.558965937039152E-4	0.0	0.011753242266882779	0.0993466626747997	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1010	0.0	28.940596	1
GTATCAA	2755	0.0	21.891108	1
GTCGCCA	250	0.0	19.980001	12
TTCGCCG	85	1.2455021E-6	19.588236	24
TCGCCAT	280	0.0	18.5	13
TACCGTA	50	0.0070352466	18.5	7
TAACCGT	130	1.3933459E-8	17.076923	7
CGGCCTT	330	0.0	16.257576	24
GTACTAA	115	1.2423498E-6	16.086956	1
ATCTCGC	335	0.0	16.014925	11
CTAACCG	130	2.5893314E-7	15.653846	6
TCTCGCC	355	0.0	15.633803	12
AGAGGTC	355	0.0	15.633803	8
CGAACTA	180	2.0190782E-10	15.416667	29
TTACGTT	75	0.0041046944	14.800001	4
AACCTCG	75	0.0041046944	14.800001	5
TTAGCCG	275	0.0	14.8	29
TATTGTC	405	0.0	14.617284	5
TATCAAC	4115	0.0	14.611179	2
GCGAACT	190	4.5474735E-10	14.605264	28
>>END_MODULE