FastQCFastQC Report
Fri 10 Feb 2017
ERR1633443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929293
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24020.25847606728986444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16400.17647824744187246No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10420.11212825233806775No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10000.10760868746455639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9310.10018368802950199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA659.387804E-825.61538512
GTATCAA15550.023.675241
GGTATCA5700.022.3947371
ATTAGAC957.1413524E-921.4210533
TCGGCGT806.9571615E-720.812511
ACACCGT450.003825265420.5555556
CGTAAAT902.1513133E-618.515
TTTGCCG1205.169568E-918.59
ATCGGCG902.1513133E-618.510
CATAATA2150.017.2093032
CGAGTCG650.001579626517.07692321
CCGTAGG650.001579626517.07692333
ACGTCGC650.001579626517.07692322
GTATTAT1201.0405347E-716.9583341
TTGCCGA1201.0405347E-716.95833410
ATCAACG21300.016.9366193
TCAACGC21450.016.9044284
CCTTATA1107.8036464E-716.8181822
ATTATAC2100.016.7380943
CCGATCG904.444483E-516.44444514
Produced by FastQC (version 0.11.5)