##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633443.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	929293
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85734208694136	33.0	33.0	33.0	33.0	33.0
2	31.973504588972478	33.0	33.0	33.0	33.0	33.0
3	31.68914002365239	33.0	33.0	33.0	27.0	33.0
4	31.97301389335764	33.0	33.0	33.0	33.0	33.0
5	32.07035778812495	33.0	33.0	33.0	33.0	33.0
6	35.247230959449816	37.0	37.0	37.0	33.0	37.0
7	35.381357655766266	37.0	37.0	37.0	33.0	37.0
8	35.50352579864477	37.0	37.0	37.0	33.0	37.0
9	35.62797847395816	37.0	37.0	37.0	33.0	37.0
10	35.631929864961855	37.0	37.0	37.0	33.0	37.0
11	35.64950559188544	37.0	37.0	37.0	33.0	37.0
12	35.637754723214314	37.0	37.0	37.0	33.0	37.0
13	35.635533679905045	37.0	37.0	37.0	33.0	37.0
14	35.613663290264746	37.0	37.0	37.0	33.0	37.0
15	35.64073225559646	37.0	37.0	37.0	33.0	37.0
16	35.6142723554358	37.0	37.0	37.0	33.0	37.0
17	35.60935463841867	37.0	37.0	37.0	33.0	37.0
18	35.60105693252828	37.0	37.0	37.0	33.0	37.0
19	35.58440341205626	37.0	37.0	37.0	33.0	37.0
20	35.58610470540508	37.0	37.0	37.0	33.0	37.0
21	35.58040144496945	37.0	37.0	37.0	33.0	37.0
22	35.4984617338127	37.0	37.0	37.0	33.0	37.0
23	35.54356806733721	37.0	37.0	37.0	33.0	37.0
24	35.536549828740775	37.0	37.0	37.0	33.0	37.0
25	35.550658403754255	37.0	37.0	37.0	33.0	37.0
26	35.44635760734236	37.0	37.0	37.0	33.0	37.0
27	35.45859271510707	37.0	37.0	37.0	33.0	37.0
28	35.48434239793047	37.0	37.0	37.0	33.0	37.0
29	35.48937418015631	37.0	37.0	37.0	33.0	37.0
30	35.48996387576362	37.0	37.0	37.0	33.0	37.0
31	35.48322326758084	37.0	37.0	37.0	33.0	37.0
32	35.48035334388616	37.0	37.0	37.0	33.0	37.0
33	35.48480941963407	37.0	37.0	37.0	33.0	37.0
34	35.46263126914762	37.0	37.0	37.0	33.0	37.0
35	35.406414338642385	37.0	37.0	37.0	33.0	37.0
36	35.444272150979295	37.0	37.0	37.0	33.0	37.0
37	35.44607459649433	37.0	37.0	37.0	33.0	37.0
38	35.43811801014319	37.0	37.0	37.0	33.0	37.0
39	35.34834653871276	37.0	37.0	37.0	33.0	37.0
40	35.15626072724103	37.0	37.0	37.0	33.0	37.0
41	35.31102461763943	37.0	37.0	37.0	33.0	37.0
42	35.36823370024309	37.0	37.0	37.0	33.0	37.0
43	35.03673114937915	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	34.0
16	37.0
17	36.0
18	29.0
19	28.0
20	65.0
21	145.0
22	438.0
23	1030.0
24	2024.0
25	3740.0
26	5927.0
27	9076.0
28	12729.0
29	17116.0
30	22356.0
31	28751.0
32	36777.0
33	48544.0
34	70922.0
35	144134.0
36	525352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.279338163528614	20.166944117732513	13.538894622040626	25.01482309669824
2	16.189834637729973	21.075053831245903	38.708028576563045	24.02708295446108
3	18.034893192997256	26.679744709149862	29.50178253790785	25.783579559945036
4	12.648432733271422	17.676986698490143	39.107256807056544	30.567323761181886
5	14.022488063506344	37.03977109480003	35.277356011505525	13.66038483018811
6	30.37136834130893	39.27006875119042	17.133778044169063	13.224784863331587
7	27.21423705978631	31.729174759736704	23.380139525424166	17.67644865505282
8	24.83619267550708	36.012538564263366	20.157797379298025	18.993471380931524
9	26.035814323361954	15.564520554873438	20.00886695584708	38.39079816591753
10	15.216083624863202	27.863009836510123	33.674094176971096	23.246812361655582
11	34.3231897797573	23.451268867838237	23.994369913471854	18.23117143893261
12	23.227980841349282	25.42470458725074	30.011847716489847	21.33546685491013
13	28.194336985213493	21.960242894329348	25.736877389585416	24.108542730871747
14	22.195260267751937	20.685617991311673	27.320554443001292	29.798567297935097
15	24.128342729365226	28.382114144839143	23.91850578880934	23.57103733698629
16	23.405858001728195	28.13805764166953	24.674026383497992	23.782057973104283
17	22.76246565937761	27.419554435468683	26.16914148713054	23.648838418023164
18	23.44685691165219	27.096297938325158	27.132669674688177	22.324175475334474
19	25.024185052507658	26.327003431641042	26.75862187706138	21.89018963878992
20	24.492060092995427	26.22854148261097	26.89915882289009	22.38023960150351
21	23.464935171146237	26.73408709631946	26.961141426869673	22.83983630566463
22	23.14027976106567	26.66446427552989	26.836961001535574	23.35829496186886
23	23.134253674567656	26.57773167343346	27.317433791064822	22.970580860934064
24	23.284690619643104	27.135252283187327	26.00826649937103	23.571790597798543
25	23.446211259527406	26.70621644626614	26.956944688058552	22.890627606147902
26	23.465150388521167	26.98137186011301	26.845677305220207	22.70780044614562
27	23.249502578842197	26.955545775121514	26.26082408885034	23.534127557185947
28	22.970688469621532	26.678130578837887	27.165705541739793	23.185475409800784
29	24.26070141494663	26.48583385433873	26.38145342749811	22.87201130321653
30	23.725132977435536	26.411583859988184	26.808337090670005	23.054946071906276
31	23.653788417646535	26.407279512489602	26.721712097261037	23.21721997260283
32	22.716193923767854	26.423420815609283	27.134606631062542	23.72577862956032
33	22.812826525111024	26.516825156328522	27.411806609971233	23.258541708589217
34	23.550484077680558	26.14363822820144	27.191208800668896	23.114668893449107
35	23.360016700868293	26.91594577813456	26.739252313317756	22.984785207679387
36	24.259840545446913	26.420300163672817	26.351861038445357	22.967998252434914
37	23.441691694653894	26.091555623468594	26.668553405653544	23.79819927622397
38	23.506794950569947	26.67899144833761	26.61786971385774	23.196343887234704
39	23.151686281936914	26.44601863997684	26.837283827597968	23.565011250488276
40	23.266181925399202	26.869243607774944	27.13891097856112	22.725663488264736
41	23.114238458699248	26.14761974963763	27.0703642446462	23.66777754701693
42	22.838006957977733	26.93811316775226	26.896683823078405	23.327196051191603
43	23.55973842480251	26.041087149047716	26.798975134860587	23.600199291289183
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	28.5
2	36.0
3	130.5
4	225.0
5	225.0
6	284.5
7	344.0
8	310.0
9	276.0
10	421.0
11	566.0
12	566.0
13	847.0
14	1128.0
15	2107.5
16	3087.0
17	3743.0
18	4399.0
19	4399.0
20	4354.5
21	4310.0
22	5109.5
23	5909.0
24	8007.0
25	10105.0
26	10105.0
27	13806.5
28	17508.0
29	21317.0
30	25126.0
31	29952.5
32	34779.0
33	34779.0
34	41950.0
35	49121.0
36	53606.0
37	58091.0
38	62311.5
39	66532.0
40	66532.0
41	69575.0
42	72618.0
43	74021.5
44	75425.0
45	74247.5
46	73070.0
47	73070.0
48	70848.0
49	68626.0
50	68242.0
51	67858.0
52	65020.5
53	62183.0
54	62183.0
55	59719.5
56	57256.0
57	50444.0
58	43632.0
59	40700.5
60	37769.0
61	37769.0
62	33781.0
63	29793.0
64	25188.5
65	20584.0
66	17502.5
67	14421.0
68	14421.0
69	12056.5
70	9692.0
71	7879.0
72	6066.0
73	4864.5
74	3663.0
75	3663.0
76	2908.5
77	2154.0
78	1671.0
79	1188.0
80	967.0
81	746.0
82	746.0
83	588.0
84	430.0
85	367.5
86	305.0
87	237.0
88	169.0
89	169.0
90	116.5
91	64.0
92	37.0
93	10.0
94	9.0
95	8.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	929293.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.561163979501174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14074565330243	52.413634044999526
2	8.837340701194082	10.880739600978576
3	2.404369560699612	4.440473663806501
4	1.013218697566219	2.4949968955188244
5	0.595126428376158	1.8318337822899766
6	0.36000217921510036	1.3297291912583136
7	0.26554086848522274	1.1442903463654566
8	0.19319560836175445	0.9514677221181963
9	0.14295311421024903	0.7920324094749749
>10	0.8844665663792703	10.416714475181383
>50	0.0962733207976523	4.1688435707825136
>100	0.06270474081738985	7.10370394887358
>500	0.003532661386849494	1.4787195416818175
>1k	5.298992080274242E-4	0.5528208066703699
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2402	0.25847606728986444	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1640	0.17647824744187246	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1042	0.11212825233806775	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1000	0.10760868746455639	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	931	0.10018368802950199	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.152173749291128E-4	0.0	0.0
2	0.0	0.0	2.152173749291128E-4	0.0	0.0
3	0.0	0.0	3.228260623936692E-4	0.0	0.0
4	0.0	0.0	5.380434373227819E-4	0.0	0.0
5	0.0	0.0	6.456521247873384E-4	0.0	0.0
6	0.0	0.0	8.608694997164512E-4	0.0	0.0
7	0.0	0.0	0.0022597824367556842	1.076086874645564E-4	0.0
8	0.0	0.0	0.002474999811684797	1.076086874645564E-4	0.0
9	0.0	0.0	0.003013043249007579	6.456521247873384E-4	0.0
10	0.0	0.0	0.003335869311401248	7.532608122518947E-4	0.0
11	0.0	0.0	0.005595651748156932	8.608694997164512E-4	0.0
12	0.0	0.0	0.006133695185479714	0.0010760868746455638	0.0
13	0.0	0.0	0.006671738622802496	0.0011836955621101202	0.0
14	0.0	0.0	0.006779347310267052	0.0011836955621101202	0.0
15	0.0	0.0	0.007424999435054391	0.0012913042495746767	0.0
16	1.076086874645564E-4	0.0	0.007855434184912616	0.001398912937039233	0.0
17	1.076086874645564E-4	0.0	0.007963042872377172	0.001398912937039233	0.0
18	1.076086874645564E-4	0.0	0.008178260247306286	0.001398912937039233	0.0
19	1.076086874645564E-4	0.0	0.008178260247306286	0.001398912937039233	0.0
20	1.076086874645564E-4	0.0	0.008393477622235399	0.0016141303119683458	0.0
21	1.076086874645564E-4	0.0	0.00860869499716451	0.001936956374362015	0.0
22	1.076086874645564E-4	0.0	0.00860869499716451	0.0021521737492911277	0.0
23	1.076086874645564E-4	0.0	0.00860869499716451	0.0023673911242202404	0.0
24	1.076086874645564E-4	0.0	0.00860869499716451	0.0032282606239366915	0.0
25	1.076086874645564E-4	0.0	0.008716303684629067	0.003335869311401248	0.0
26	1.076086874645564E-4	0.0	0.008716303684629067	0.0036586953737949173	0.0
27	1.076086874645564E-4	0.0	0.008716303684629067	0.0045195648735113685	0.0
28	1.076086874645564E-4	0.0	0.008716303684629067	0.009792390559274632	0.0
29	1.076086874645564E-4	0.0	0.008716303684629067	0.020553259305730272	0.0
30	1.076086874645564E-4	0.0	0.008716303684629067	0.036909779800342844	0.0
31	1.076086874645564E-4	0.0	0.008716303684629067	0.08576412390925144	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGTA	65	9.387804E-8	25.615385	12
GTATCAA	1555	0.0	23.67524	1
GGTATCA	570	0.0	22.394737	1
ATTAGAC	95	7.1413524E-9	21.421053	3
TCGGCGT	80	6.9571615E-7	20.8125	11
ACACCGT	45	0.0038252654	20.555555	6
CGTAAAT	90	2.1513133E-6	18.5	15
TTTGCCG	120	5.169568E-9	18.5	9
ATCGGCG	90	2.1513133E-6	18.5	10
CATAATA	215	0.0	17.209303	2
CGAGTCG	65	0.0015796265	17.076923	21
CCGTAGG	65	0.0015796265	17.076923	33
ACGTCGC	65	0.0015796265	17.076923	22
GTATTAT	120	1.0405347E-7	16.958334	1
TTGCCGA	120	1.0405347E-7	16.958334	10
ATCAACG	2130	0.0	16.936619	3
TCAACGC	2145	0.0	16.904428	4
CCTTATA	110	7.8036464E-7	16.818182	2
ATTATAC	210	0.0	16.738094	3
CCGATCG	90	4.444483E-5	16.444445	14
>>END_MODULE