FastQCFastQC Report
Fri 10 Feb 2017
ERR1633442.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633442.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644323
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27570.42789098014505145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.31319695246017915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10810.1677729958421475No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8540.1325422187319093No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7320.11360761605592226No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6570.10196749146002858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA303.597739E-430.8333326
TCGCTAA250.005494573629.59999814
GGTATCA4250.027.423531
TGCGGTA451.3223306E-424.66666636
GTATCAA13350.022.4494361
ATAACGC502.7003168E-422.1999993
GACCCGC705.0961808E-621.14285916
GCCGCTC1001.2871169E-820.34999827
TAACGGC2250.019.73333436
AACGGCC2300.019.30434837
GTATTAG3200.019.0781251
TTAACGG2350.018.89361835
GCACCGC2400.018.510
CACCGCC2400.018.511
GTGACAC2400.018.524
TGTCCCA801.614874E-518.510
ATTAGAG2750.018.1636353
TATTAGA2700.017.8148162
CCGCTCT953.6014117E-617.52631628
GAGGCAC2550.017.4117647
Produced by FastQC (version 0.11.5)