##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633442.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644323
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.857166359108707	33.0	33.0	33.0	33.0	33.0
2	31.974286188759365	33.0	33.0	33.0	33.0	33.0
3	31.685533497950562	33.0	33.0	33.0	27.0	33.0
4	31.973857832174236	33.0	33.0	33.0	33.0	33.0
5	32.076242816103104	33.0	33.0	33.0	33.0	33.0
6	35.23021528022436	37.0	37.0	37.0	33.0	37.0
7	35.371718842878494	37.0	37.0	37.0	33.0	37.0
8	35.50283165431003	37.0	37.0	37.0	33.0	37.0
9	35.60376705472255	37.0	37.0	37.0	33.0	37.0
10	35.60264649872812	37.0	37.0	37.0	33.0	37.0
11	35.618477068178535	37.0	37.0	37.0	33.0	37.0
12	35.6322248313346	37.0	37.0	37.0	33.0	37.0
13	35.61746670536361	37.0	37.0	37.0	33.0	37.0
14	35.57869733037622	37.0	37.0	37.0	33.0	37.0
15	35.60697196902796	37.0	37.0	37.0	33.0	37.0
16	35.60489381878344	37.0	37.0	37.0	33.0	37.0
17	35.59432458565036	37.0	37.0	37.0	33.0	37.0
18	35.572172652536075	37.0	37.0	37.0	33.0	37.0
19	35.56741416960127	37.0	37.0	37.0	33.0	37.0
20	35.56759109949513	37.0	37.0	37.0	33.0	37.0
21	35.572793459181185	37.0	37.0	37.0	33.0	37.0
22	35.482787359755896	37.0	37.0	37.0	33.0	37.0
23	35.53985656262465	37.0	37.0	37.0	33.0	37.0
24	35.52426034768276	37.0	37.0	37.0	33.0	37.0
25	35.52620968054842	37.0	37.0	37.0	33.0	37.0
26	35.42538602533202	37.0	37.0	37.0	33.0	37.0
27	35.4461535596277	37.0	37.0	37.0	33.0	37.0
28	35.46179633506797	37.0	37.0	37.0	33.0	37.0
29	35.4646644617684	37.0	37.0	37.0	33.0	37.0
30	35.467290473877235	37.0	37.0	37.0	33.0	37.0
31	35.46195153672925	37.0	37.0	37.0	33.0	37.0
32	35.451888261011945	37.0	37.0	37.0	33.0	37.0
33	35.44211831643446	37.0	37.0	37.0	33.0	37.0
34	35.42298971168187	37.0	37.0	37.0	33.0	37.0
35	35.36338296165122	37.0	37.0	37.0	33.0	37.0
36	35.39411909865083	37.0	37.0	37.0	33.0	37.0
37	35.39309942373623	37.0	37.0	37.0	33.0	37.0
38	35.38167037339968	37.0	37.0	37.0	33.0	37.0
39	35.28558192086888	37.0	37.0	37.0	33.0	37.0
40	35.07360749189459	37.0	37.0	37.0	33.0	37.0
41	35.24234894610312	37.0	37.0	37.0	33.0	37.0
42	35.282349070264445	37.0	37.0	37.0	33.0	37.0
43	34.96377127620774	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	27.0
16	29.0
17	22.0
18	29.0
19	17.0
20	38.0
21	104.0
22	323.0
23	690.0
24	1446.0
25	2691.0
26	4312.0
27	6356.0
28	9234.0
29	12185.0
30	15998.0
31	20302.0
32	25622.0
33	34184.0
34	49293.0
35	100238.0
36	361181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.932420540629465	20.61885110418222	13.730225368332343	24.718502986855974
2	15.918723994021633	21.390979369043166	38.92752548023274	23.76277115670246
3	17.623924646489417	27.38533313260585	29.904721700141078	25.086020520763654
4	12.378884503579727	17.875661741083277	39.99717532976473	29.748278425572266
5	13.627947473549757	37.339967687014116	35.620022876724875	13.412061962711249
6	29.262031620786466	40.17658845020277	17.432405796471645	13.128974132539115
7	26.46141764301445	32.15033453718089	23.831370290987593	17.55687752881707
8	24.457453792585397	35.8452204872401	20.664325190936843	19.033000529237665
9	25.95902986545568	15.499058701924346	20.323502342769075	38.218409089850894
10	15.084359862987974	27.96920178233588	33.98342135854222	22.963016996133927
11	33.64756496353537	23.89531337543437	24.261744497713103	18.195377163317154
12	22.86011829470623	25.951424984053027	30.156769198057496	21.031687523183248
13	27.581042427478142	22.133464116599903	26.409269884824848	23.876223571097103
14	21.86015399108832	21.004682434120774	27.976961865399808	29.158201709391097
15	23.94978915854315	28.598078913836694	24.63531489641065	22.816817031209503
16	22.820697072741467	28.387315057820377	25.196058498610167	23.595929370827985
17	22.156278760807854	27.719637510999917	26.585578972037315	23.53850475615491
18	22.946720821699675	27.272501524856324	27.731277635595813	22.049500017848192
19	24.64850703761933	26.294265453817417	27.496302320420035	21.560925188143216
20	23.84254481059965	26.509219754688257	27.62620611091021	22.02202932380188
21	22.978847565584342	27.02231644687525	27.66997297939077	22.32886300814964
22	22.786863110582736	26.834367235066885	27.535878744046077	22.842890910304302
23	22.658821740027904	26.71191312431808	28.100967992761394	22.52829714289262
24	22.883398543898014	27.354292800350134	26.71874199741434	23.04356665833751
25	22.948428039973738	26.97932558670108	27.83541795031374	22.23682842301144
26	22.911490044589435	27.187295812814384	27.76185236286769	22.13936177972849
27	22.87812168741454	27.093088404418282	26.984602443184553	23.044187464982624
28	22.359592937082798	26.798981256295367	28.233044606509466	22.608381200112365
29	23.74212933575241	26.62888023553404	27.143839347656378	22.48515108105717
30	23.34822751942737	26.595667080020423	27.572195932785267	22.483909467766942
31	22.97589873402005	26.60513438135842	27.782338982156464	22.63662790246507
32	22.16791888540375	26.582630140473025	27.892842564986815	23.356608409136413
33	22.212151358868145	26.767630520717095	28.141475626355106	22.878742494059658
34	22.980554783858405	26.653557299677338	27.82688185894342	22.539006057520837
35	22.94951445160269	27.11155740211043	27.48047175096962	22.458456395317256
36	23.636437004421694	26.864631559016207	26.943163599623173	22.555767836938927
37	22.915214884460124	26.39933697850302	27.46417557653537	23.22127256050149
38	23.084229493592503	26.834367235066885	27.28600406938756	22.795399201953057
39	22.3726298766302	26.906846410883983	27.6907700020021	23.029753710483718
40	22.717177564668653	27.15222023736542	27.915812410856045	22.214789787109883
41	22.50951774187791	26.374194309375888	27.811672096138118	23.304615852608087
42	22.225188298415546	27.458743518390623	27.495526312113643	22.820541871080188
43	23.01097430946901	26.248325762078956	27.627447724200437	23.113252204251594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	37.5
2	46.0
3	173.0
4	300.0
5	300.0
6	371.5
7	443.0
8	391.0
9	339.0
10	500.5
11	662.0
12	662.0
13	1061.5
14	1461.0
15	2593.5
16	3726.0
17	4243.5
18	4761.0
19	4761.0
20	4648.0
21	4535.0
22	5084.0
23	5633.0
24	7118.5
25	8604.0
26	8604.0
27	10974.5
28	13345.0
29	16137.5
30	18930.0
31	22025.0
32	25120.0
33	25120.0
34	30233.5
35	35347.0
36	38542.0
37	41737.0
38	44406.5
39	47076.0
40	47076.0
41	48924.0
42	50772.0
43	51605.5
44	52439.0
45	51619.0
46	50799.0
47	50799.0
48	48585.0
49	46371.0
50	46052.5
51	45734.0
52	43295.5
53	40857.0
54	40857.0
55	39577.5
56	38298.0
57	33509.0
58	28720.0
59	26166.0
60	23612.0
61	23612.0
62	21034.5
63	18457.0
64	15165.5
65	11874.0
66	9908.5
67	7943.0
68	7943.0
69	6651.5
70	5360.0
71	4581.5
72	3803.0
73	3497.5
74	3192.0
75	3192.0
76	2612.0
77	2032.0
78	1555.5
79	1079.0
80	730.5
81	382.0
82	382.0
83	305.5
84	229.0
85	185.5
86	142.0
87	111.5
88	81.0
89	81.0
90	59.5
91	38.0
92	25.5
93	13.0
94	7.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	644323.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.69222992927335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.4695072539369	51.615577088729715
2	7.882664846267169	9.410676849175601
3	2.0448663927569077	3.6618790467326745
4	0.910681032094735	2.174423262401076
5	0.5272661550808863	1.5736846281506085
6	0.33556498128545237	1.201837321146212
7	0.24084054251021064	1.00634163278673
8	0.18518543200262658	0.8843305109322082
9	0.147571633556748	0.7927991893177085
>10	1.0473272623929302	12.34235368664873
>50	0.12532533462208373	5.182599556047026
>100	0.08003967272783256	8.327895125933262
>500	0.002369595574177381	0.9052572406603824
>1k	7.898651913924603E-4	0.9203448613380554
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2757	0.42789098014505145	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2018	0.31319695246017915	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1081	0.1677729958421475	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	854	0.1325422187319093	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	732	0.11360761605592226	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	657	0.10196749146002858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	3.1040332255716463E-4	1.5520166127858231E-4	0.0
4	0.0	0.0	6.208066451143293E-4	1.5520166127858231E-4	0.0
5	1.5520166127858231E-4	0.0	6.208066451143293E-4	1.5520166127858231E-4	0.0
6	1.5520166127858231E-4	0.0	0.0010864116289500763	1.5520166127858231E-4	0.0
7	1.5520166127858231E-4	0.0	0.0031040332255716464	1.5520166127858231E-4	0.0
8	3.1040332255716463E-4	0.0	0.004500848177078887	1.5520166127858231E-4	0.0
9	3.1040332255716463E-4	0.0	0.004966453160914634	4.6560498383574697E-4	0.0
10	6.208066451143293E-4	0.0	0.005742461467307546	4.6560498383574697E-4	0.0
11	7.760083063929116E-4	0.0	0.009467301337993521	4.6560498383574697E-4	0.0
12	7.760083063929116E-4	0.0	0.010243309644386434	4.6560498383574697E-4	0.0
13	7.760083063929116E-4	0.0	0.011174519612057928	4.6560498383574697E-4	0.0
14	7.760083063929116E-4	0.0	0.011640124595893675	4.6560498383574697E-4	0.0
15	7.760083063929116E-4	0.0	0.012105729579729422	6.208066451143293E-4	0.0
16	7.760083063929116E-4	0.0	0.013192141208679498	6.208066451143293E-4	0.0
17	7.760083063929116E-4	0.0	0.01334734286995808	6.208066451143293E-4	0.0
18	7.760083063929116E-4	0.0	0.01396814951507241	6.208066451143293E-4	0.0
19	7.760083063929116E-4	0.0	0.014433754498908156	7.760083063929116E-4	0.0
20	7.760083063929116E-4	0.0	0.01474415782146532	7.760083063929116E-4	0.0
21	7.760083063929116E-4	0.0	0.015054561144022486	0.0010864116289500763	0.0
22	7.760083063929116E-4	0.0	0.015209762805301067	0.0010864116289500763	0.0
23	7.760083063929116E-4	0.0	0.01536496446657965	0.0017072182740644056	0.0
24	7.760083063929116E-4	0.0	0.015520166127858233	0.0017072182740644056	0.0
25	7.760083063929116E-4	0.0	0.015520166127858233	0.0021728232579001526	0.0
26	7.760083063929116E-4	0.0	0.015520166127858233	0.0026384282417358995	0.0
27	7.760083063929116E-4	0.0	0.015520166127858233	0.0029488315642930644	0.0
28	9.312099676714939E-4	0.0	0.015520166127858233	0.00931209967671494	0.0
29	9.312099676714939E-4	0.0	0.015520166127858233	0.02328024919178735	0.0
30	9.312099676714939E-4	0.0	0.015520166127858233	0.04345646515800305	0.0
31	0.0010864116289500763	0.0	0.015520166127858233	0.09824265158934262	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	30	3.597739E-4	30.833332	6
TCGCTAA	25	0.0054945736	29.599998	14
GGTATCA	425	0.0	27.42353	1
TGCGGTA	45	1.3223306E-4	24.666666	36
GTATCAA	1335	0.0	22.449436	1
ATAACGC	50	2.7003168E-4	22.199999	3
GACCCGC	70	5.0961808E-6	21.142859	16
GCCGCTC	100	1.2871169E-8	20.349998	27
TAACGGC	225	0.0	19.733334	36
AACGGCC	230	0.0	19.304348	37
GTATTAG	320	0.0	19.078125	1
TTAACGG	235	0.0	18.893618	35
GCACCGC	240	0.0	18.5	10
CACCGCC	240	0.0	18.5	11
GTGACAC	240	0.0	18.5	24
TGTCCCA	80	1.614874E-5	18.5	10
ATTAGAG	275	0.0	18.163635	3
TATTAGA	270	0.0	17.814816	2
CCGCTCT	95	3.6014117E-6	17.526316	28
GAGGCAC	255	0.0	17.411764	7
>>END_MODULE