FastQCFastQC Report
Fri 10 Feb 2017
ERR1633441.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633441.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977475
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39730.4064554080667025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28150.2879869050359344No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG27620.2825647714775314No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA25790.2638430650400266No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG24780.25351031995703216No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG23180.23714161487506077No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG19940.20399498708406866No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG17120.17514514437709403No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC16860.1724852298012737No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA16600.16982531522545335No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT15990.16358474641295173No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT15970.1633801375994271No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC15710.16072022302360675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15630.15990178776950817No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC15490.1584695260748357No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT15170.1551957850584414No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG14100.14424921353487302No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC13930.14251003861991354No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA12900.13197268472339446No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12430.12716437760556537No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA11780.12051459116601448No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG11030.11284176065884038No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG10730.10977262845597074No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG10550.10793114913424896No Hit
CATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCC10510.10752193150719969No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA10490.10731732269367504No Hit
CAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAG10450.10690810506662574No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10300.10537353896519093No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT10250.10486201693137931No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG10120.10353205964346915No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG10030.10261131998260825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAC358.868441E-426.42857228
GGTATCA9550.024.0209431
AACGCAC400.001930981523.12532
GTATCAA24750.022.8727281
CGAGTCG903.8271537E-922.6111121
TTAACGG2550.022.49019635
AACGGCC2700.021.24074237
CGAACGC450.003825404720.55555530
ACGAACG450.003825404720.55555529
CGCCGTC951.6754711E-719.47368426
TGTCCGG1254.129106E-1019.2414
TTTAACG3100.019.09677334
ATATAGC2000.018.56
GCGGATA500.00703414818.519
GCGTCAA500.00703414818.526
TCTATCG500.00703414818.531
CTAGCGC609.2354655E-418.528
TAACGGC3250.017.64615436
GGACCGT852.7224416E-517.4117646
GTTTAAC3450.017.1594233
Produced by FastQC (version 0.11.5)