##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633441.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	977475
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.828476431622292	33.0	33.0	33.0	27.0	33.0
2	31.929617637279726	33.0	33.0	33.0	33.0	33.0
3	31.650761400547328	33.0	33.0	33.0	27.0	33.0
4	31.94038210695926	33.0	33.0	33.0	33.0	33.0
5	32.04239699225044	33.0	33.0	33.0	33.0	33.0
6	35.197716565641066	37.0	37.0	37.0	33.0	37.0
7	35.34216527276912	37.0	37.0	37.0	33.0	37.0
8	35.475032098007624	37.0	37.0	37.0	33.0	37.0
9	35.60250031970127	37.0	37.0	37.0	33.0	37.0
10	35.60154684263025	37.0	37.0	37.0	33.0	37.0
11	35.62080360111512	37.0	37.0	37.0	33.0	37.0
12	35.60002455305762	37.0	37.0	37.0	33.0	37.0
13	35.59118340622522	37.0	37.0	37.0	33.0	37.0
14	35.58019284380675	37.0	37.0	37.0	33.0	37.0
15	35.6140862937671	37.0	37.0	37.0	33.0	37.0
16	35.59176961047597	37.0	37.0	37.0	33.0	37.0
17	35.578941660912044	37.0	37.0	37.0	33.0	37.0
18	35.56169006879971	37.0	37.0	37.0	33.0	37.0
19	35.557745210874955	37.0	37.0	37.0	33.0	37.0
20	35.5548653418246	37.0	37.0	37.0	33.0	37.0
21	35.553549707153635	37.0	37.0	37.0	33.0	37.0
22	35.46066958234226	37.0	37.0	37.0	33.0	37.0
23	35.49808230389524	37.0	37.0	37.0	33.0	37.0
24	35.500813831555796	37.0	37.0	37.0	33.0	37.0
25	35.516817309905626	37.0	37.0	37.0	33.0	37.0
26	35.41018644978132	37.0	37.0	37.0	33.0	37.0
27	35.41548888718381	37.0	37.0	37.0	33.0	37.0
28	35.45099670068288	37.0	37.0	37.0	33.0	37.0
29	35.44962889076447	37.0	37.0	37.0	33.0	37.0
30	35.453251489807926	37.0	37.0	37.0	33.0	37.0
31	35.447330110744524	37.0	37.0	37.0	33.0	37.0
32	35.44112841760659	37.0	37.0	37.0	33.0	37.0
33	35.441822041484436	37.0	37.0	37.0	33.0	37.0
34	35.42888667229341	37.0	37.0	37.0	33.0	37.0
35	35.346780224558174	37.0	37.0	37.0	33.0	37.0
36	35.39280697716054	37.0	37.0	37.0	33.0	37.0
37	35.37932632548147	37.0	37.0	37.0	33.0	37.0
38	35.3836671014604	37.0	37.0	37.0	33.0	37.0
39	35.28970971124581	37.0	37.0	37.0	33.0	37.0
40	35.083397529348574	37.0	37.0	37.0	33.0	37.0
41	35.25290979308934	37.0	37.0	37.0	33.0	37.0
42	35.29627253893962	37.0	37.0	37.0	33.0	37.0
43	34.972748152126655	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	22.0
16	50.0
17	31.0
18	34.0
19	30.0
20	64.0
21	184.0
22	447.0
23	1128.0
24	2177.0
25	4069.0
26	6621.0
27	9882.0
28	14071.0
29	18883.0
30	24384.0
31	31047.0
32	39619.0
33	52553.0
34	75388.0
35	150998.0
36	545792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.686641602087015	19.82495716002967	12.836747742909028	23.651653494974294
2	16.90917926289675	21.668277961073173	38.47771042737666	22.944832348653417
3	18.517711450420727	26.18992813115425	29.194199340136578	26.098161078288445
4	12.184761758612753	17.595744136678686	39.13010562929998	31.08938847540858
5	14.661346837515026	35.93759431187498	34.78646512698534	14.614593723624644
6	30.75781989309189	38.99301772423847	16.874088851377273	13.375073531292362
7	28.962070641192874	31.09266221642497	22.144402670145016	17.80086447223714
8	25.333844855367143	34.92334842323333	20.168086140310493	19.574720581089032
9	26.3323358653674	14.804061484948464	19.870891838665948	38.992710811018185
10	15.653085756668968	27.395585564848208	33.34387068722985	23.60745799125297
11	34.85623673239725	23.71569605360751	22.94493465306018	18.48313256093506
12	23.790992096984578	25.013529757794316	29.714928770556792	21.480549374664314
13	28.59674160464462	22.012020767794574	25.761784188853937	23.62945343870687
14	22.99035780966265	20.72850968055449	26.885598097138036	29.395534412644825
15	24.041944806772552	28.351415637228573	24.91889818153917	22.687741374459705
16	24.494846415509347	27.82710555257168	24.117547763369906	23.560500268549067
17	23.96879715593749	26.502570398219905	24.99020435305251	24.538428092790095
18	23.38699199468017	25.714212639709455	27.912529732218218	22.986265633392158
19	26.919256246962835	25.431750172638683	26.4987851351697	21.150208445228778
20	25.876262820020973	25.781017417325252	26.44967901992378	21.893040742729994
21	23.62311056548761	26.82380623545359	26.293460190797717	23.259623008261084
22	24.419754980945804	25.530473925164326	26.65479935548224	23.394971738407634
23	22.91495946187882	26.16885342336121	27.632829484130028	23.28335763062994
24	23.48735261771401	27.334202920790812	25.59942709532213	23.579017366173048
25	23.615335430573673	27.006419601524335	27.089286171001813	22.288958796900175
26	25.097317066932657	26.07923476303742	25.78521189800251	23.03823627202742
27	24.090641704391416	26.02347886135195	25.419678252640733	24.466201181615897
28	23.555487352617714	25.845980715619326	27.24468656487378	23.35384536688918
29	24.867336760530957	26.770607943937186	26.415202434844883	21.946852860686974
30	24.63377579989258	25.87257986137753	26.18992813115425	23.303716207575643
31	23.645822143788845	25.246067674365076	26.941149390009976	24.166960791836107
32	22.624619555487353	26.11146065116755	26.787692779866497	24.476227013478606
33	23.41512570653981	25.447198138059797	27.431392107215018	23.706284048185374
34	24.45883526432901	25.654569170567022	27.116089925573544	22.770505639530423
35	24.2362208752142	26.569375175835695	25.58305839024016	23.611345558709942
36	24.100872145067648	25.884549476968722	25.62244558684365	24.392132791119977
37	24.212179339625052	25.676871531241208	26.69490268293307	23.41604644620067
38	23.696155911915906	25.393283715696054	26.129875444384766	24.780684928003275
39	23.40018926315251	25.715440292590603	27.05603723880406	23.828333205452825
40	24.30670861147344	26.49540908974654	26.219801017928845	22.978081280851175
41	23.65216501700811	25.67523466073301	26.806209877490474	23.86639044476841
42	22.707690733778357	27.884293715951813	26.014373769150108	23.393641781119722
43	24.952965548991024	24.960536075091436	25.752474487838562	24.334023888078978
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30.0
1	53.5
2	77.0
3	258.0
4	439.0
5	439.0
6	532.0
7	625.0
8	582.5
9	540.0
10	715.0
11	890.0
12	890.0
13	1426.0
14	1962.0
15	3519.5
16	5077.0
17	5834.5
18	6592.0
19	6592.0
20	6024.0
21	5456.0
22	6243.5
23	7031.0
24	9026.5
25	11022.0
26	11022.0
27	13332.5
28	15643.0
29	18729.5
30	21816.0
31	26045.5
32	30275.0
33	30275.0
34	41021.0
35	51767.0
36	56280.5
37	60794.0
38	62273.5
39	63753.0
40	63753.0
41	64103.5
42	64454.0
43	67804.0
44	71154.0
45	69702.0
46	68250.0
47	68250.0
48	66017.5
49	63785.0
50	67366.5
51	70948.0
52	73995.5
53	77043.0
54	77043.0
55	75473.0
56	73903.0
57	62087.5
58	50272.0
59	46772.5
60	43273.0
61	43273.0
62	40375.5
63	37478.0
64	28735.0
65	19992.0
66	17135.0
67	14278.0
68	14278.0
69	12640.5
70	11003.0
71	11985.5
72	12968.0
73	11778.5
74	10589.0
75	10589.0
76	6196.5
77	1804.0
78	1390.5
79	977.0
80	781.0
81	585.0
82	585.0
83	477.5
84	370.0
85	338.0
86	306.0
87	230.5
88	155.0
89	155.0
90	116.5
91	78.0
92	48.0
93	18.0
94	10.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	977475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.26559286528149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.54276322752673	40.43229419283452
2	8.304821636521023	7.850646365811668
3	2.126834486482862	3.01578278789817
4	0.9844900366848004	1.8613002101547926
5	0.5609415508385879	1.3256617481578148
6	0.3697799657128936	1.048672158547403
7	0.25589745936812713	0.8466601590827665
8	0.20169092654076823	0.762643297479788
9	0.16540046025762736	0.7035975732840469
>10	1.1406464903865072	10.792931356218837
>50	0.1645672226715531	5.460341957023469
>100	0.15730564488351476	15.153136589562743
>500	0.018040647382738334	5.512301793979833
>1k	0.006820244742254736	5.234029809964099
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3973	0.4064554080667025	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2815	0.2879869050359344	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	2762	0.2825647714775314	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	2579	0.2638430650400266	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	2478	0.25351031995703216	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	2318	0.23714161487506077	No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1994	0.20399498708406866	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	1712	0.17514514437709403	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1686	0.1724852298012737	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	1660	0.16982531522545335	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	1599	0.16358474641295173	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	1597	0.1633801375994271	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	1571	0.16072022302360675	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1563	0.15990178776950817	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	1549	0.1584695260748357	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	1517	0.1551957850584414	No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG	1410	0.14424921353487302	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	1393	0.14251003861991354	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	1290	0.13197268472339446	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1243	0.12716437760556537	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	1178	0.12051459116601448	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	1103	0.11284176065884038	No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG	1073	0.10977262845597074	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	1055	0.10793114913424896	No Hit
CATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCC	1051	0.10752193150719969	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	1049	0.10731732269367504	No Hit
CAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAG	1045	0.10690810506662574	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1030	0.10537353896519093	No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT	1025	0.10486201693137931	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	1012	0.10353205964346915	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	1003	0.10261131998260825	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.0460881352464257E-4	0.0	0.0
2	0.0	0.0	3.069132202869639E-4	0.0	0.0
3	0.0	0.0	4.0921762704928513E-4	0.0	0.0
4	0.0	0.0	6.138264405739278E-4	2.0460881352464257E-4	0.0
5	0.0	0.0	8.184352540985703E-4	2.0460881352464257E-4	0.0
6	0.0	0.0	0.0019437837284841043	2.0460881352464257E-4	0.0
7	0.0	0.0	0.003478349829918924	2.0460881352464257E-4	0.0
8	0.0	0.0	0.0041944806772551725	2.0460881352464257E-4	0.0
9	1.0230440676232128E-4	0.0	0.004706002711066779	4.0921762704928513E-4	0.0
10	2.0460881352464257E-4	0.0	0.005012915931353743	5.115220338116064E-4	0.0
11	2.0460881352464257E-4	0.0	0.007672830507174096	7.161308473362491E-4	0.0
12	2.0460881352464257E-4	0.0	0.008082048134223382	7.161308473362491E-4	0.0
13	2.0460881352464257E-4	0.0	0.008491265761272666	7.161308473362491E-4	0.0
14	2.0460881352464257E-4	0.0	0.00869587457479731	7.161308473362491E-4	0.0
15	2.0460881352464257E-4	0.0	0.009309701015371237	7.161308473362491E-4	0.0
16	2.0460881352464257E-4	0.0	0.010741962710043735	8.184352540985703E-4	0.0
17	2.0460881352464257E-4	0.0	0.01115118033709302	0.0010230440676232129	0.0
18	2.0460881352464257E-4	0.0	0.011458093557379985	0.001125348474385534	0.0
19	2.0460881352464257E-4	0.0	0.011560397964142306	0.001125348474385534	0.0
20	2.0460881352464257E-4	0.0	0.012174224404716234	0.0012276528811478555	0.0
21	2.0460881352464257E-4	0.0	0.012788050845290161	0.001739174914959462	0.0
22	2.0460881352464257E-4	0.0	0.012890355252052482	0.002148392542008747	0.0
23	2.0460881352464257E-4	0.0	0.013197268472339445	0.002762218982582675	0.0
24	2.0460881352464257E-4	0.0	0.013197268472339445	0.003478349829918924	0.0
25	2.0460881352464257E-4	0.0	0.013197268472339445	0.0038875674569682087	0.0
26	3.069132202869639E-4	0.0	0.013197268472339445	0.004296785084017494	0.0
27	3.069132202869639E-4	0.0	0.013197268472339445	0.0057290467786899925	0.0
28	3.069132202869639E-4	0.0	0.013197268472339445	0.011765006777666948	0.0
29	3.069132202869639E-4	0.0	0.013197268472339445	0.02782679863935139	0.0
30	4.0921762704928513E-4	0.0	0.013197268472339445	0.05084529016087368	0.0
31	4.0921762704928513E-4	0.0	0.013197268472339445	0.11192102099797949	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAC	35	8.868441E-4	26.428572	28
GGTATCA	955	0.0	24.020943	1
AACGCAC	40	0.0019309815	23.125	32
GTATCAA	2475	0.0	22.872728	1
CGAGTCG	90	3.8271537E-9	22.61111	21
TTAACGG	255	0.0	22.490196	35
AACGGCC	270	0.0	21.240742	37
CGAACGC	45	0.0038254047	20.555555	30
ACGAACG	45	0.0038254047	20.555555	29
CGCCGTC	95	1.6754711E-7	19.473684	26
TGTCCGG	125	4.129106E-10	19.24	14
TTTAACG	310	0.0	19.096773	34
ATATAGC	200	0.0	18.5	6
GCGGATA	50	0.007034148	18.5	19
GCGTCAA	50	0.007034148	18.5	26
TCTATCG	50	0.007034148	18.5	31
CTAGCGC	60	9.2354655E-4	18.5	28
TAACGGC	325	0.0	17.646154	36
GGACCGT	85	2.7224416E-5	17.411764	6
GTTTAAC	345	0.0	17.15942	33
>>END_MODULE