FastQCFastQC Report
Fri 10 Feb 2017
ERR1633440.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633440.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754427
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22520.29850469296565474No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCA20930.27742909519410097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.19856129221250035No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCA14900.19750088477745362No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCA14190.18808976879141387No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCTTGTTCTTCTGGGCA11700.1550845873755844No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT10210.13533449889783902No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG9400.12459787361799088No Hit
CTCTACCACCATGAATCCACTCCTGATCCTTACCTTTGTGGCA9310.12340491525356331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9060.12009114201904227No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG8150.1080290074453857No Hit
CCTTAATCCAGTCCACATAGTTGTAGACCTTGGTGTAGACTCC7920.10498033606962634No Hit
CTTTATTGGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCT7750.10272697027015205No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT7660.10153401190572447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAG358.8665565E-426.42857228
GACGGTT809.767973E-1025.437529
GTCTTAT451.3226233E-424.6666661
GTATCAA11750.023.3021281
CTCGGTA400.001930575523.12527
GACGTAT1850.023.026
ACGTATG1950.021.82051327
CGAATCA603.7241014E-521.58333426
ACGGTTT957.1358954E-921.42105330
GACAGCG2100.021.14285737
TTAACGG1351.8189894E-1220.55555335
GGTATCA5800.020.4137941
GCCGCAA555.140841E-420.18181834
AGCCTTA1750.020.0857147
AGACGTA2150.019.79069725
CAGACGT2250.019.73333424
GCTCTAG1800.019.5277771
AAGCCTT1900.019.4736846
TGACGGT1153.0449883E-919.30434828
TAACGGC1457.2759576E-1219.1379336
Produced by FastQC (version 0.11.5)