FastQCFastQC Report
Fri 10 Feb 2017
ERR1633439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862588
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT142341.6501504774005666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101781.179937583179919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56910.6597587724382904No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA54350.6300806410476381No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA49820.5775642601102728No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG41970.4865590525256554No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA37590.4357816245994611No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT34820.4036689589931694No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT29910.3467472304275042No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC29140.3378206049701596No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT27450.3182284010442992No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC24610.2853042240327943No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC24350.28229003881343123No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23580.27336341335608655No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG23270.26976957713299976No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT22530.2611907422778893No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC22380.2594517892667183No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA21350.2475109785900105No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA21300.24693132758628686No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC21050.24403307256766846No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA20850.24171446855277373No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA20040.23232412229245014No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC19860.23023737867904492No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA18910.21922400960829505No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG18490.21435494117701617No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA18140.21029738415095037No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG17820.20658761772711887No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA17690.20508052511743732No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG17610.2041530835114794No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC17540.20334157210626624No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT17450.20229820029956364No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC17380.20148668889435048No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC17350.20113889829211626No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC17220.19963180568243472No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG16790.19464680705041107No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA16630.19279192383849533No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT16630.19279192383849533No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG16470.19093704062657954No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG15890.18421308898338487No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG15800.18316971717668226No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA15470.179344020552106No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT15100.17505460312455076No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG14950.17331565011337974No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG14900.17273599910965606No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG14880.17250413870816658No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA14590.16914216288656928No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT14160.16415716425454563No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.16369344345156667No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC14040.1627660018456088No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT13930.16149076963741668No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA13710.1589403052210325No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG13610.15778100321358518No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT13250.1536075159867747No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG13110.15198449317634838No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC13020.15094112136964577No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG12960.15024554016517735No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG12940.15001367976368787No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC12130.1406233335033643No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT11950.13853658988995907No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG11860.13749321808325643No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC11660.1351746140683617No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT11480.13308787045495646No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG11430.1325082194512328No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC11380.13192856844750914No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG11310.13111705704229598No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11040.1279869416221881No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC10980.1272913604177197No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG10920.1265957792132513No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10900.12636391881176182No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC10820.12543647720580392No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT10770.12485682620208025No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG10660.12358159399388817No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA10550.12230636178569607No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA10500.12172671078197238No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG10480.12149485038048292No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG10470.12137892017973818No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT10450.12114705977824872No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA10300.11940810676707768No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC10230.11859659536186452No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA10220.1184806651611198No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA9930.11511868933952246No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA9900.11477089873728825No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG9890.11465496853654351No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA9810.11372752693058563No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT9800.11361159672984089No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG9740.11291601552537248No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG9630.11164078331718039No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGA9590.11117706251420145No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG9550.11071334171122252No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG9360.10851066789707253No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9170.10630799408292255No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA9080.10526462227621992No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA9080.10526462227621992No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC8940.10364159946579363No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG8910.10329380886355943No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA8870.10283008806058049No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC8680.1006274142464305No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA8680.1006274142464305No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC8650.1002796236441963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAGT250.00549559329.59999819
GAGGTGT358.867594E-426.428576
AACGCCC358.867594E-426.4285720
TAGGCAG358.867594E-426.428575
GGTATCA29850.025.1624771
AGGTCCG1103.6379788E-1223.5454548
AAGACTC400.001930798223.12500231
TACTAAT400.001930798223.1250022
GTATCAA76800.022.6914081
TTGCTCT1155.456968E-1222.5217416
GGACGTA3250.022.19999927
GACACCC603.7248974E-521.58333426
GGACACC603.7248974E-521.58333425
CTTTATG1301.8189894E-1221.3461531
AAACACG3350.020.98507315
ACGAAAG3350.020.98507319
TCTCGCG1151.3460522E-1020.91304420
GACCGTT3100.020.8870967
CCGGCAG4350.020.8390816
GTCCGCT1251.8189894E-1120.7210
Produced by FastQC (version 0.11.5)