FastQCFastQC Report
Fri 10 Feb 2017
ERR1633438.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633438.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936772
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43660.4660685844581179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29740.3174731951851678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17430.18606448527496552No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10810.11539627572130677No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9770.10429432135033924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9550.025.7643971
GTATCAA27250.024.0330281
GCGTAAT450.003825287820.5555551
ATCGGCG656.90039E-519.92307510
ATTGGGC1052.2580934E-819.3809533
CGGCGTA701.2188919E-418.512
CATTCGC500.007033933418.523
AACGGCC1800.018.537
CGAACTA2050.018.04878229
GCGAACT2100.017.61904728
TTAACGG1900.017.52631635
TTTGGCG852.7222453E-517.41176422
TCGGCGT752.0667519E-417.26666611
TATACGC650.001579639517.0769217
TAACCGT2000.016.6500027
TGGTCTA904.444544E-516.44444535
TAACGGC2050.016.24390436
TGCGGTA803.381729E-416.187536
CGGGATA1151.2415185E-616.08695617
GCCAACG1151.2415185E-616.08695631
Produced by FastQC (version 0.11.5)