FastQCFastQC Report
Fri 10 Feb 2017
ERR1633437.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633437.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241496
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40150.3234001559408971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.2124050339268109No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC22620.18219953990991514No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG20110.16198199591460624No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC18210.14667787894604575No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA17750.14297267167997318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16320.1314543099615303No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC16040.12919896640826875No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG15320.12339951155702475No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC15050.12122471598780826No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG14200.11437813734397856No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT13620.10970635426936534No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA13620.10970635426936534No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG12750.10269867965744554No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG12560.10116826796058949No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC12470.10044333610418398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10850.025.2350231
ACAACGC551.9028179E-523.5454543
GTATCAA28850.022.5719221
CGGCCTA500.007035193518.4999985
TGCGGTA1258.580173E-917.7636
GTGACAC5850.017.70940224
CACATGT6500.017.07692328
GCGTCAA1201.0413714E-716.95833226
TATTAGA7300.016.7260272
AACGATT1452.9795046E-916.58620522
ATTAGAG6850.016.4744533
CAACCCA6750.016.44444517
AGTGACA6300.016.44444523
TAGGACC3500.016.3857154
GCCGGCA1708.54925E-1116.3235315
TTAACGG6350.016.3149635
GTCAACC6900.016.08695615
TCAACCC6800.016.05147216
CTAGTAC700.002592650215.8571433
TACCCTC7700.015.8571425
Produced by FastQC (version 0.11.5)