##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633437.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1241496
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91104038998112	33.0	33.0	33.0	33.0	33.0
2	32.01369879564654	33.0	33.0	33.0	33.0	33.0
3	31.72246386617436	33.0	33.0	33.0	27.0	33.0
4	32.000955299090776	33.0	33.0	33.0	33.0	33.0
5	32.09838452963199	33.0	33.0	33.0	33.0	33.0
6	35.277840605205334	37.0	37.0	37.0	33.0	37.0
7	35.424938944628096	37.0	37.0	37.0	33.0	37.0
8	35.54618621405143	37.0	37.0	37.0	33.0	37.0
9	35.665551882567485	37.0	37.0	37.0	33.0	37.0
10	35.66526190982492	37.0	37.0	37.0	33.0	37.0
11	35.68276257031839	37.0	37.0	37.0	33.0	37.0
12	35.679257122052746	37.0	37.0	37.0	33.0	37.0
13	35.676370282304575	37.0	37.0	37.0	33.0	37.0
14	35.651973908897006	37.0	37.0	37.0	33.0	37.0
15	35.6704387287595	37.0	37.0	37.0	33.0	37.0
16	35.66246447833904	37.0	37.0	37.0	33.0	37.0
17	35.6512787797947	37.0	37.0	37.0	33.0	37.0
18	35.6365135288394	37.0	37.0	37.0	33.0	37.0
19	35.62952840766301	37.0	37.0	37.0	33.0	37.0
20	35.62448771482148	37.0	37.0	37.0	33.0	37.0
21	35.630681049314695	37.0	37.0	37.0	33.0	37.0
22	35.53693769452338	37.0	37.0	37.0	33.0	37.0
23	35.58860117148988	37.0	37.0	37.0	33.0	37.0
24	35.57540660622346	37.0	37.0	37.0	33.0	37.0
25	35.59044169292531	37.0	37.0	37.0	33.0	37.0
26	35.48934511267052	37.0	37.0	37.0	33.0	37.0
27	35.50838021226005	37.0	37.0	37.0	33.0	37.0
28	35.5364004394698	37.0	37.0	37.0	33.0	37.0
29	35.5446243886408	37.0	37.0	37.0	33.0	37.0
30	35.53967552050107	37.0	37.0	37.0	33.0	37.0
31	35.53327115028965	37.0	37.0	37.0	33.0	37.0
32	35.52887645228015	37.0	37.0	37.0	33.0	37.0
33	35.53217972510584	37.0	37.0	37.0	33.0	37.0
34	35.513706044965105	37.0	37.0	37.0	33.0	37.0
35	35.4538025092308	37.0	37.0	37.0	33.0	37.0
36	35.49542245806672	37.0	37.0	37.0	33.0	37.0
37	35.49335398583644	37.0	37.0	37.0	33.0	37.0
38	35.47985333823065	37.0	37.0	37.0	33.0	37.0
39	35.3861591177096	37.0	37.0	37.0	33.0	37.0
40	35.188871329428366	37.0	37.0	37.0	33.0	37.0
41	35.363364843704694	37.0	37.0	37.0	33.0	37.0
42	35.40587082036511	37.0	37.0	37.0	33.0	37.0
43	35.075851231095385	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	34.0
16	56.0
17	35.0
18	32.0
19	31.0
20	63.0
21	150.0
22	509.0
23	1229.0
24	2485.0
25	4500.0
26	7581.0
27	11270.0
28	16106.0
29	21998.0
30	29364.0
31	37505.0
32	48579.0
33	64481.0
34	92824.0
35	191977.0
36	710681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64507980694259	19.930148788236128	13.538021870388627	24.886749534432653
2	16.482453427155626	20.891247333861727	38.874792991681005	23.75150624730164
3	17.81487817922893	27.178178584546387	29.46775503102708	25.5391882051976
4	12.57184880176819	17.61987150985585	39.53568920077068	30.272590487605278
5	13.936170555523336	36.94212466250394	35.29685154039965	13.82485324157307
6	29.782375456707072	39.66835173049289	17.12176277652123	13.427510036278811
7	27.357075657110453	31.681294180569246	23.224319691726755	17.737310470593542
8	24.76415550271608	35.45093983387784	20.1154896995238	19.669414963882286
9	26.417725067177017	15.369521931605096	19.771549807651414	38.44120319356647
10	15.829934208406632	27.291267954145642	33.256410008570306	23.622387828877418
11	34.21541430661074	23.530240935129875	23.68151004916649	18.572834709092902
12	23.902694813354213	25.601532344848472	29.082655119307677	21.41311772248964
13	27.9696430757731	21.904540973148524	25.5847783641671	24.541037586911273
14	22.422706154510365	20.636071320406995	27.219096960441274	29.722125564641367
15	24.63221790485028	27.925905520436633	24.41425506002436	23.027621514688732
16	23.46354720434057	27.704479112296777	24.671364225096173	24.160609458266478
17	22.90462474305193	27.05800099235116	25.630449071120648	24.40692519347626
18	23.60378124456301	26.757154271942884	27.116076088847645	22.52298839464646
19	25.859366441776693	25.461862140514345	26.61087913291706	22.067892284791895
20	24.848891981931477	25.677489093802958	26.956671628422484	22.516947295843078
21	23.855332598735718	26.553448420292934	26.75787920379929	22.833339777172057
22	23.488758723346674	26.361502574313572	26.65389175639712	23.495846945942635
23	23.34288632424108	26.368671344893578	27.3677079910044	22.920734339860942
24	23.75400323480704	26.980030543795554	25.519534497090607	23.746431724306806
25	23.68392648868784	26.426263153485795	26.936292988459087	22.95351736936728
26	23.77873146590887	26.609832009124474	26.967867798204747	22.643568726761906
27	23.69568649435842	26.48836564918453	25.98244376139754	23.83350409505951
28	22.93249434553152	26.203145237680992	27.51325819817382	23.351102218613672
29	24.85155006540496	25.952802103268958	26.198231810654242	22.997416020671835
30	24.03680720678923	25.862346717186362	26.945233814688084	23.155612261336323
31	23.664111684612756	26.064602705123498	26.956832724390573	23.314452885873173
32	22.606838846037363	25.87918124585178	27.16150515184906	24.3524747562618
33	22.826332102560137	26.1044739572258	27.57302480233525	23.496169137878816
34	23.72202568514115	25.948855252050752	27.201698595887542	23.127420466920555
35	23.754567070695355	26.56996075702217	26.4709672846308	23.204504887651673
36	24.48167372267007	26.261059238209388	26.10246025762467	23.154806781495875
37	23.56858177553532	25.875234394633573	26.427551921230513	24.128631908600592
38	23.95795073040912	26.191626875962548	26.358119558983677	23.492302834644654
39	23.081024828110603	26.272094312023558	26.891186117393858	23.755694742471984
40	23.476515429771823	26.71220849684574	27.015713300727512	22.795562772654925
41	23.28094492451043	25.544021084240303	27.142818019550607	24.032215971698662
42	22.796207156527288	26.935648604586724	26.739030975532746	23.529113263353242
43	23.936847158589313	25.369956890718935	26.799441963566533	23.89375398712521
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	39.5
2	54.0
3	196.0
4	338.0
5	338.0
6	443.0
7	548.0
8	510.5
9	473.0
10	632.5
11	792.0
12	792.0
13	1298.5
14	1805.0
15	3295.5
16	4786.0
17	5535.5
18	6285.0
19	6285.0
20	5735.5
21	5186.0
22	5474.5
23	5763.0
24	8086.0
25	10409.0
26	10409.0
27	14287.0
28	18165.0
29	22895.5
30	27626.0
31	34266.0
32	40906.0
33	40906.0
34	52080.5
35	63255.0
36	70003.0
37	76751.0
38	83285.0
39	89819.0
40	89819.0
41	95113.0
42	100407.0
43	103687.0
44	106967.0
45	103875.0
46	100783.0
47	100783.0
48	96971.0
49	93159.0
50	93530.0
51	93901.0
52	89459.5
53	85018.0
54	85018.0
55	83741.5
56	82465.0
57	71012.5
58	59560.0
59	54454.0
60	49348.0
61	49348.0
62	44325.5
63	39303.0
64	31851.0
65	24399.0
66	20499.0
67	16599.0
68	16599.0
69	13970.5
70	11342.0
71	9826.0
72	8310.0
73	7799.5
74	7289.0
75	7289.0
76	6165.5
77	5042.0
78	3730.0
79	2418.0
80	1659.0
81	900.0
82	900.0
83	714.5
84	529.0
85	467.5
86	406.0
87	310.0
88	214.0
89	214.0
90	169.0
91	124.0
92	73.5
93	23.0
94	13.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1241496.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.051173007597896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.3062213025118	39.196554311137206
2	9.420468172736388	8.864881556159789
3	2.5634504346198717	3.6184004968710473
4	1.1685489924526862	2.199264032469822
5	0.6717464630601593	1.5803229525342763
6	0.4298842166295171	1.213593398992266
7	0.3118184142746487	1.026999550989393
8	0.24037858401731513	0.904807547513608
9	0.1876338953965194	0.7945555386952045
>10	1.3322330687604638	12.655312137120678
>50	0.19113441784623297	6.249756938781656
>100	0.16099199757881436	14.777778133239467
>500	0.010442723673473563	3.3920641365883997
>1k	0.005047316442178889	3.5257092689072707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4015	0.3234001559408971	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2637	0.2124050339268109	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	2262	0.18219953990991514	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	2011	0.16198199591460624	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1821	0.14667787894604575	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1775	0.14297267167997318	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1632	0.1314543099615303	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1604	0.12919896640826875	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1532	0.12339951155702475	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1505	0.12122471598780826	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1420	0.11437813734397856	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1362	0.10970635426936534	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1362	0.10970635426936534	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	1275	0.10269867965744554	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1256	0.10116826796058949	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1247	0.10044333610418398	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.4164395213516595E-4	0.0	0.0
2	0.0	0.0	3.221919361802213E-4	0.0	0.0
3	0.0	0.0	4.832879042703319E-4	0.0	0.0
4	0.0	0.0	4.832879042703319E-4	1.6109596809011064E-4	0.0
5	0.0	0.0	5.638358883153872E-4	1.6109596809011064E-4	0.0
6	0.0	0.0	0.0012082197606758298	1.6109596809011064E-4	0.0
7	0.0	0.0	0.003221919361802213	4.027399202252766E-4	0.0
8	0.0	0.0	0.004107947186297822	4.027399202252766E-4	0.0
9	0.0	0.0	0.004832879042703319	0.0012887677447208851	0.0
10	8.054798404505532E-5	0.0	0.005316166946973651	0.0014498637128109958	0.0
11	1.6109596809011064E-4	0.0	0.007974250420460476	0.0015304116968560511	0.0
12	1.6109596809011064E-4	0.0	0.008296442356640697	0.0016109596809011065	0.0
13	1.6109596809011064E-4	0.0	0.008699182276865974	0.0017720556489912171	0.0
14	1.6109596809011064E-4	0.0	0.009182470181136307	0.0017720556489912171	0.0
15	1.6109596809011064E-4	0.0	0.009746306069451694	0.0017720556489912171	0.0
16	1.6109596809011064E-4	0.0	0.010229593973722025	0.0017720556489912171	0.0
17	2.4164395213516595E-4	0.0	0.010712881877992358	0.0017720556489912171	0.0
18	2.4164395213516595E-4	0.0	0.010793429862037412	0.0017720556489912171	0.0
19	2.4164395213516595E-4	0.0	0.01103507381417258	0.0018526036330362725	0.0
20	2.4164395213516595E-4	0.0	0.011437813734397855	0.002013699601126383	0.0
21	3.221919361802213E-4	0.0	0.011840553654623132	0.0021747955692164936	0.0
22	4.027399202252766E-4	0.0	0.012001649622713243	0.0024164395213516596	0.0
23	4.832879042703319E-4	0.0	0.012162745590803353	0.0025775354894417703	0.0
24	4.832879042703319E-4	0.0	0.012243293574848409	0.002980275409667047	0.0
25	4.832879042703319E-4	0.0	0.012243293574848409	0.0036246592820274896	0.0
26	4.832879042703319E-4	0.0	0.012243293574848409	0.004430139122478043	0.0
27	4.832879042703319E-4	0.0	0.012243293574848409	0.0056383588831538725	0.0
28	4.832879042703319E-4	0.0	0.012243293574848409	0.01256548551102863	0.0
29	4.832879042703319E-4	0.0	0.012243293574848409	0.027225218607228697	0.0
30	5.638358883153872E-4	0.0	0.012243293574848409	0.05114796986861013	0.0
31	5.638358883153872E-4	0.0	0.012243293574848409	0.10865923047677963	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1085	0.0	25.235023	1
ACAACGC	55	1.9028179E-5	23.545454	3
GTATCAA	2885	0.0	22.571922	1
CGGCCTA	50	0.0070351935	18.499998	5
TGCGGTA	125	8.580173E-9	17.76	36
GTGACAC	585	0.0	17.709402	24
CACATGT	650	0.0	17.076923	28
GCGTCAA	120	1.0413714E-7	16.958332	26
TATTAGA	730	0.0	16.726027	2
AACGATT	145	2.9795046E-9	16.586205	22
ATTAGAG	685	0.0	16.474453	3
CAACCCA	675	0.0	16.444445	17
AGTGACA	630	0.0	16.444445	23
TAGGACC	350	0.0	16.385715	4
GCCGGCA	170	8.54925E-11	16.32353	15
TTAACGG	635	0.0	16.31496	35
GTCAACC	690	0.0	16.086956	15
TCAACCC	680	0.0	16.051472	16
CTAGTAC	70	0.0025926502	15.857143	3
TACCCTC	770	0.0	15.857142	5
>>END_MODULE