FastQCFastQC Report
Fri 10 Feb 2017
ERR1633436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239242
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74540.6014967213829099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51610.4164642579899648No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA31610.25507527988883527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29150.23522443558239634No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27810.22441137404962067No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA20420.1647781466412533No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG20030.16163106156828125No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT19720.15912953240771374No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT17560.14169952277279177No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC16570.13371076835678586No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15970.12886909901375196No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13520.10909894919636358No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC13140.10603255861244211No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12750.10288547353947008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.10223991762706558No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12570.10143297273655992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.024.6666661
GTATCAA45250.023.1403331
TATACTG1600.020.81255
GACCGTA500.007035186518.4999987
TAACCGT1002.8753675E-718.4999987
ACCGTGC1258.580173E-917.7600029
CTACGGG4050.017.35802511
TTAACGG650.001580028317.07692335
CGACTCG1352.2195309E-816.44444534
CCTACGG4200.016.29761910
GTATAGA1251.6578997E-716.281
AGACCGT803.382832E-416.18756
TTAGTAC803.382832E-416.18753
GGACGTA1151.2423097E-616.08695627
TACCCGA1403.4733603E-815.85714330
CAACGAT700.002592646315.85714321
GACCGTT2100.015.8571427
ATTACGC1302.5892405E-715.6538463
TAGGACC2850.015.5789474
TATCAAC67100.015.5223542
Produced by FastQC (version 0.11.5)