##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633436.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1239242
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.893072539503986	33.0	33.0	33.0	33.0	33.0
2	32.005367797411644	33.0	33.0	33.0	33.0	33.0
3	31.72585015678939	33.0	33.0	33.0	27.0	33.0
4	31.994337667703324	33.0	33.0	33.0	33.0	33.0
5	32.09473371625558	33.0	33.0	33.0	33.0	33.0
6	35.25458627128519	37.0	37.0	37.0	33.0	37.0
7	35.41756493082062	37.0	37.0	37.0	33.0	37.0
8	35.56217268297879	37.0	37.0	37.0	33.0	37.0
9	35.67523615242221	37.0	37.0	37.0	33.0	37.0
10	35.66440372421206	37.0	37.0	37.0	33.0	37.0
11	35.6886992209754	37.0	37.0	37.0	33.0	37.0
12	35.68134956691268	37.0	37.0	37.0	33.0	37.0
13	35.668552227894146	37.0	37.0	37.0	33.0	37.0
14	35.65343734315009	37.0	37.0	37.0	33.0	37.0
15	35.68945371444803	37.0	37.0	37.0	33.0	37.0
16	35.67325429577112	37.0	37.0	37.0	33.0	37.0
17	35.65773190385736	37.0	37.0	37.0	33.0	37.0
18	35.63372529336481	37.0	37.0	37.0	33.0	37.0
19	35.61638727544741	37.0	37.0	37.0	33.0	37.0
20	35.62209721749263	37.0	37.0	37.0	33.0	37.0
21	35.61707156471456	37.0	37.0	37.0	33.0	37.0
22	35.51461780669151	37.0	37.0	37.0	33.0	37.0
23	35.55875849914706	37.0	37.0	37.0	33.0	37.0
24	35.55898767149596	37.0	37.0	37.0	33.0	37.0
25	35.58062912651443	37.0	37.0	37.0	33.0	37.0
26	35.47958832899466	37.0	37.0	37.0	33.0	37.0
27	35.49135439244312	37.0	37.0	37.0	33.0	37.0
28	35.50676784679667	37.0	37.0	37.0	33.0	37.0
29	35.510035973603216	37.0	37.0	37.0	33.0	37.0
30	35.52143649101628	37.0	37.0	37.0	33.0	37.0
31	35.5180440946966	37.0	37.0	37.0	33.0	37.0
32	35.50628771458682	37.0	37.0	37.0	33.0	37.0
33	35.50379748265472	37.0	37.0	37.0	33.0	37.0
34	35.47640412445672	37.0	37.0	37.0	33.0	37.0
35	35.413262300664435	37.0	37.0	37.0	33.0	37.0
36	35.43392331764095	37.0	37.0	37.0	33.0	37.0
37	35.43434938454313	37.0	37.0	37.0	33.0	37.0
38	35.41532485180457	37.0	37.0	37.0	33.0	37.0
39	35.315945553814345	37.0	37.0	37.0	33.0	37.0
40	35.10522077205259	37.0	37.0	37.0	33.0	37.0
41	35.28173754601603	37.0	37.0	37.0	33.0	37.0
42	35.30649300136697	37.0	37.0	37.0	33.0	37.0
43	34.963304181104256	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	30.0
16	43.0
17	40.0
18	37.0
19	39.0
20	55.0
21	171.0
22	482.0
23	1180.0
24	2531.0
25	4548.0
26	7710.0
27	11442.0
28	16443.0
29	22788.0
30	30124.0
31	38324.0
32	49498.0
33	65002.0
34	93998.0
35	190614.0
36	704138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.979982925046116	19.20432006016581	13.616468776881351	24.19922823790672
2	16.764522183721986	21.79888996660862	38.26968421018655	23.166903639482843
3	19.184630604837473	26.31527982427968	29.686534187834173	24.81355538304867
4	12.757395246449038	17.65974684524895	38.7987979748911	30.784059933410905
5	15.014581494171436	35.79704367669914	33.69809932200491	15.490275507124515
6	29.58308385287135	38.89651900113134	17.0953695888293	14.42502755716801
7	27.11237998712116	32.26173741690485	21.458278528326186	19.167604067647805
8	25.573939553372142	33.63806262215128	20.19306963450238	20.594928189974194
9	27.33856663992989	14.505560657240474	19.219813402063522	38.93605930076611
10	16.11355974055108	26.90975612511519	32.097201353730746	24.87948278060298
11	35.15600665568145	23.177313228570366	23.02334814346189	18.643331972286283
12	24.11320791257882	24.874399027792794	29.36004428513559	21.652348774492793
13	30.283834795786458	21.96423297467323	24.52813897527682	23.223793254263494
14	23.16875961273101	20.964186171869578	27.380769857703335	28.486284357696075
15	25.480898807496843	27.182826276062304	24.12749083714077	23.208784079300088
16	23.0536892713449	27.242217420003517	24.506028685276966	25.19806462337461
17	23.318609278897906	26.301561761141084	25.29336481494333	25.08646414501768
18	24.985918811660675	24.660558631808797	26.898458896648116	23.45506365988241
19	26.728032135773322	25.06072260301055	26.381287916323043	21.82995734489309
20	26.481591166212894	24.195354902432292	26.530572721066587	22.792481210288223
21	24.94508740020109	25.319913301840963	26.448990592636466	23.28600870532148
22	23.99071367820006	25.661412379502956	26.679776831321085	23.668097110975904
23	23.98328978520741	25.464033659285274	26.90556001168456	23.647116543822754
24	23.98062686706874	25.343556787132783	26.355062207381607	24.32075413841687
25	24.484160478744265	25.640028339904553	26.74199228237907	23.133818898972113
26	24.61085082655365	25.468391161694004	26.351673038841483	23.569084972910858
27	24.545246206955543	25.484368670526013	26.784276194641564	23.18610892787688
28	23.856276659441818	25.841764562530965	26.303094956433043	23.998863821594167
29	24.64441973399869	25.397622094796656	26.074971635887103	23.882986535317556
30	24.53499800684612	25.234215754469265	26.92113404807132	23.309652190613296
31	24.72818061363317	24.91958794166111	26.32827163701682	24.023959807688893
32	22.98179048160085	25.491389091073412	26.87812388540737	24.648696541918365
33	23.59046901250926	25.388342228555842	27.30088231354328	23.720306445391618
34	23.23734992842399	25.60508762614566	26.585122195664766	24.57244024976558
35	23.924624891667648	25.8173948268377	26.497165202599653	23.760815078895
36	24.489082842576348	25.202422125783343	26.587785113803434	23.72070991783687
37	24.777081473997814	25.277790778556568	26.133152362492556	23.81197538495306
38	24.285894119147027	25.518825217350603	25.816749270925293	24.378531392577074
39	23.84925623889442	25.200324069068024	26.780160775699986	24.170258916337566
40	24.16622419188504	25.893166952056173	26.417196963950545	23.52341189210824
41	24.074474557834545	24.782084532318947	27.237779223105736	23.905661686740764
42	23.620083889990816	25.489775201292403	27.003523121391947	23.88661778732483
43	23.889442094441602	25.372526108701933	26.62837444179587	24.109657355060595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	52.0
2	77.0
3	356.5
4	636.0
5	636.0
6	794.5
7	953.0
8	934.0
9	915.0
10	1276.5
11	1638.0
12	1638.0
13	2500.5
14	3363.0
15	6292.0
16	9221.0
17	10476.5
18	11732.0
19	11732.0
20	10225.0
21	8718.0
22	8782.5
23	8847.0
24	10782.5
25	12718.0
26	12718.0
27	17109.0
28	21500.0
29	24442.5
30	27385.0
31	30898.0
32	34411.0
33	34411.0
34	40939.5
35	47468.0
36	49676.0
37	51884.0
38	56620.0
39	61356.0
40	61356.0
41	67519.0
42	73682.0
43	79135.5
44	84589.0
45	88324.5
46	92060.0
47	92060.0
48	98153.0
49	104246.0
50	104200.0
51	104154.0
52	105976.0
53	107798.0
54	107798.0
55	101109.0
56	94420.0
57	86389.0
58	78358.0
59	70562.5
60	62767.0
61	62767.0
62	54505.5
63	46244.0
64	38096.0
65	29948.0
66	25611.0
67	21274.0
68	21274.0
69	17958.0
70	14642.0
71	11950.5
72	9259.0
73	7454.0
74	5649.0
75	5649.0
76	4385.0
77	3121.0
78	2427.5
79	1734.0
80	1371.0
81	1008.0
82	1008.0
83	816.0
84	624.0
85	523.0
86	422.0
87	329.0
88	236.0
89	236.0
90	178.5
91	121.0
92	76.5
93	32.0
94	18.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1239242.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.1944829418399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.02079360131621	36.96519185008493
2	10.899487482255243	10.069923771476745
3	3.3029527597101023	4.57734584748394
4	1.5475274565214714	2.8594892276924
5	0.9000381066691772	2.0788397482767595
6	0.5865825579714566	1.6258126780915958
7	0.4072304187920365	1.3168259044000934
8	0.2982511639130605	1.1022046643012615
9	0.25115582529045627	1.0441812138411335
>10	1.4628328563047845	13.034907838630108
>50	0.17590667882159233	5.741646281525445
>100	0.13177010905062342	11.900720342552953
>500	0.010669643713068963	3.4759447054575556
>1k	0.004445684880445402	3.1706885130512537
>5k	3.556547904356321E-4	1.0362774131338814
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7454	0.6014967213829099	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5161	0.4164642579899648	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	3161	0.25507527988883527	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2915	0.23522443558239634	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2781	0.22441137404962067	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	2042	0.1647781466412533	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	2003	0.16163106156828125	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1972	0.15912953240771374	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1756	0.14169952277279177	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1657	0.13371076835678586	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1597	0.12886909901375196	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1352	0.10909894919636358	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1314	0.10603255861244211	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1275	0.10288547353947008	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1267	0.10223991762706558	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1257	0.10143297273655992	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.4208346715169434E-4	0.0	0.0
2	0.0	0.0	6.455559124045183E-4	0.0	0.0
3	0.0	0.0	0.001129722846707907	0.0	0.0
4	0.0	0.0	0.001452500802910166	8.069448905056478E-5	0.0
5	0.0	0.0	0.0020173622262641194	8.069448905056478E-5	0.0
6	0.0	0.0	0.002582223649618073	8.069448905056478E-5	0.0
7	0.0	0.0	0.005890697700691229	2.4208346715169434E-4	0.0
8	0.0	0.0	0.006859031569298006	2.4208346715169434E-4	0.0
9	8.069448905056478E-5	0.0	0.008069448905056478	4.034724452528239E-4	0.0
10	4.841669343033887E-4	0.0	0.008472921350309302	4.841669343033887E-4	0.0
11	5.648614233539535E-4	0.0	0.01331459069334319	8.069448905056478E-4	0.0
12	5.648614233539535E-4	0.0	0.014605702518152226	9.683338686067774E-4	0.0
13	5.648614233539535E-4	0.0	0.015735425364860132	9.683338686067774E-4	0.0
14	6.455559124045183E-4	0.0	0.017026537189669167	0.0010490283576573421	0.0
15	6.455559124045183E-4	0.0	0.018236954525427642	0.0010490283576573421	0.0
16	6.455559124045183E-4	0.0	0.019366677372135547	0.0010490283576573421	0.0
17	6.455559124045183E-4	0.0	0.01977014981738837	0.001129722846707907	0.0
18	6.455559124045183E-4	0.0	0.019850844306438936	0.0012104173357584717	0.0
19	6.455559124045183E-4	0.0	0.02025431675169176	0.0012104173357584717	0.0
20	6.455559124045183E-4	0.0	0.020899872664096278	0.0013718063138596012	0.0
21	6.455559124045183E-4	0.0	0.02194890102175362	0.0019366677372135547	0.0
22	6.455559124045183E-4	0.0	0.022190984488905316	0.0020980567153146843	0.0
23	6.455559124045183E-4	0.0	0.02243306795605701	0.002259445693415814	0.0
24	6.455559124045183E-4	0.0	0.02243306795605701	0.0031470850729720264	0.0
25	6.455559124045183E-4	0.0	0.022513762445107575	0.0037926409853765446	0.0
26	6.455559124045183E-4	0.0	0.02259445693415814	0.004518891386831628	0.0
27	6.455559124045183E-4	0.0	0.02259445693415814	0.006052086678792358	0.0
28	6.455559124045183E-4	0.0	0.02259445693415814	0.016461675766315215	0.0
29	6.455559124045183E-4	0.0	0.02259445693415814	0.04026655003623183	0.0
30	6.455559124045183E-4	0.0	0.02259445693415814	0.0723829566783566	0.0
31	6.455559124045183E-4	0.0	0.02259445693415814	0.14702535905012903	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1680	0.0	24.666666	1
GTATCAA	4525	0.0	23.140333	1
TATACTG	160	0.0	20.8125	5
GACCGTA	50	0.0070351865	18.499998	7
TAACCGT	100	2.8753675E-7	18.499998	7
ACCGTGC	125	8.580173E-9	17.760002	9
CTACGGG	405	0.0	17.358025	11
TTAACGG	65	0.0015800283	17.076923	35
CGACTCG	135	2.2195309E-8	16.444445	34
CCTACGG	420	0.0	16.297619	10
GTATAGA	125	1.6578997E-7	16.28	1
AGACCGT	80	3.382832E-4	16.1875	6
TTAGTAC	80	3.382832E-4	16.1875	3
GGACGTA	115	1.2423097E-6	16.086956	27
TACCCGA	140	3.4733603E-8	15.857143	30
CAACGAT	70	0.0025926463	15.857143	21
GACCGTT	210	0.0	15.857142	7
ATTACGC	130	2.5892405E-7	15.653846	3
TAGGACC	285	0.0	15.578947	4
TATCAAC	6710	0.0	15.522354	2
>>END_MODULE