FastQCFastQC Report
Fri 10 Feb 2017
ERR1633435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262248
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46320.3669643366438291No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31690.2510600135631033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17590.13935454839302577No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14870.11780569270064202No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13120.10394153922208631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.025.375481
GTATCAA33150.022.3785821
TTAACGG2250.021.37777735
AACGGCC2400.020.812537
TCGCGCT450.003826012620.55555723
TAACGGC2550.018.86274536
CATAATA2900.018.52
GCGTAAT500.00703525518.51
TTCTGCG1653.6379788E-1217.93939418
TAGGACC3200.017.9218754
CGAACTA3650.017.23287629
AATACGC650.0015800517.0769235
ATAATAT3550.016.6760563
GCGAACT3800.016.55263128
TGCGGGT1951.8189894E-1216.12820621
ACGTATA700.002592682415.85714329
GTACCCT2350.015.7446811
AAAGCGA4000.015.72525
TAACCGT2400.015.4166677
CATGCTA4100.015.3414644
Produced by FastQC (version 0.11.5)