##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633435.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1262248
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.902787724757733	33.0	33.0	33.0	33.0	33.0
2	32.0044587117587	33.0	33.0	33.0	33.0	33.0
3	31.730347760503484	33.0	33.0	33.0	27.0	33.0
4	32.00692653107789	33.0	33.0	33.0	33.0	33.0
5	32.11006394939822	33.0	33.0	33.0	33.0	33.0
6	35.27805550097921	37.0	37.0	37.0	33.0	37.0
7	35.41333794943625	37.0	37.0	37.0	33.0	37.0
8	35.54241797174565	37.0	37.0	37.0	33.0	37.0
9	35.64942388500516	37.0	37.0	37.0	33.0	37.0
10	35.65643597771595	37.0	37.0	37.0	33.0	37.0
11	35.67232746655174	37.0	37.0	37.0	33.0	37.0
12	35.668770320887816	37.0	37.0	37.0	33.0	37.0
13	35.66777685526141	37.0	37.0	37.0	33.0	37.0
14	35.646792072556266	37.0	37.0	37.0	33.0	37.0
15	35.674922043845584	37.0	37.0	37.0	33.0	37.0
16	35.66065543379748	37.0	37.0	37.0	33.0	37.0
17	35.6486348166129	37.0	37.0	37.0	33.0	37.0
18	35.62775223252483	37.0	37.0	37.0	33.0	37.0
19	35.616929478200795	37.0	37.0	37.0	33.0	37.0
20	35.61805920864997	37.0	37.0	37.0	33.0	37.0
21	35.61727410144441	37.0	37.0	37.0	33.0	37.0
22	35.530333183336396	37.0	37.0	37.0	33.0	37.0
23	35.57139563699051	37.0	37.0	37.0	33.0	37.0
24	35.56521935467515	37.0	37.0	37.0	33.0	37.0
25	35.592857346575315	37.0	37.0	37.0	33.0	37.0
26	35.482078006857606	37.0	37.0	37.0	33.0	37.0
27	35.50428679625557	37.0	37.0	37.0	33.0	37.0
28	35.516972100569774	37.0	37.0	37.0	33.0	37.0
29	35.52644092127696	37.0	37.0	37.0	33.0	37.0
30	35.529234350143554	37.0	37.0	37.0	33.0	37.0
31	35.52572156977076	37.0	37.0	37.0	33.0	37.0
32	35.520055488303406	37.0	37.0	37.0	33.0	37.0
33	35.515435952364356	37.0	37.0	37.0	33.0	37.0
34	35.4985137627471	37.0	37.0	37.0	33.0	37.0
35	35.436050601783485	37.0	37.0	37.0	33.0	37.0
36	35.47557215380812	37.0	37.0	37.0	33.0	37.0
37	35.48199719864876	37.0	37.0	37.0	33.0	37.0
38	35.467880321458225	37.0	37.0	37.0	33.0	37.0
39	35.36978470158004	37.0	37.0	37.0	33.0	37.0
40	35.163265855838155	37.0	37.0	37.0	33.0	37.0
41	35.34586705623617	37.0	37.0	37.0	33.0	37.0
42	35.390061224101764	37.0	37.0	37.0	33.0	37.0
43	35.071293438373445	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	37.0
16	60.0
17	47.0
18	29.0
19	37.0
20	76.0
21	189.0
22	514.0
23	1246.0
24	2539.0
25	4689.0
26	7872.0
27	11865.0
28	16803.0
29	22717.0
30	29885.0
31	38440.0
32	49646.0
33	64913.0
34	94254.0
35	193257.0
36	723126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.634449014773644	20.095337841691965	13.662925193781254	24.60728794975314
2	16.207987653773266	21.578168474024125	39.09818038927374	23.11566348292887
3	17.73985777755243	27.167561366704483	30.095908252577942	24.996672603165145
4	12.209248895621146	18.14999904931519	39.94706270083216	29.693689354231502
5	13.724640482694367	36.915724960546584	35.31223658108391	14.047397975675144
6	28.965860908474404	40.206758101419055	17.499968310506333	13.327412679600206
7	26.791882419302702	32.2702036366863	23.148145213935774	17.78976873007523
8	24.705921498786292	35.222159195340375	20.6156793276757	19.45623997819763
9	26.608717145917442	15.241616544450853	20.100091265741757	38.04957504388995
10	15.363224976391326	27.70113321629347	33.6264347418257	23.309207065489506
11	33.85499521488646	23.761178468890424	24.1353521653431	18.248474150880018
12	23.331152039852707	25.897525684334617	29.98135073297799	20.78997154283469
13	28.49344978165939	22.356858557113974	25.747792826766215	23.40189883446042
14	22.329209473891027	21.229742491174473	27.744230927678238	28.696817107256262
15	24.228836171655647	28.385784726931636	24.666071960502215	22.7193071409105
16	22.73024001622502	28.327317611119206	25.176510479715553	23.765931892940216
17	22.640241854215652	27.257321857511364	26.186375419093554	23.916060869179432
18	23.572625981582064	26.724700692732334	27.477326167282502	22.225347158403103
19	25.58760243628827	25.903467464396858	27.120740139814046	21.388189959500828
20	24.964983109499876	26.037038680195966	27.214620264797407	21.783357945506747
21	23.759435546738832	26.78800045632871	27.01038147812474	22.442182518807716
22	22.87609091081943	26.77437397405264	27.3396353173069	23.00989979782103
23	22.996827881684105	26.57322491301234	27.768631837800495	22.661315367503057
24	23.080725816162907	26.920462539849538	26.70299338957162	23.29581825441593
25	23.32322966643639	26.84836894176105	27.356272301481166	22.472129090321395
26	23.410454997750048	26.84638834840697	27.140308402152353	22.602848251690634
27	23.325527154727123	26.841555700623015	26.841476476888854	22.991440667761008
28	22.900967163346664	26.74007009715999	27.431534848936185	22.927427890557166
29	23.90180059703006	26.664886773439132	26.763044980067306	22.670267649463497
30	23.468446771157492	26.443614883921384	27.386614991665663	22.70132335325546
31	23.505998821150836	26.37659160481934	27.09166502937616	23.025744544653666
32	22.36676152388437	26.4046368067131	27.42448393659566	23.804117732806866
33	22.473396670068006	26.444407121263012	28.074277004202024	23.00791920446695
34	23.119228550966213	26.27455143521717	27.4099859932438	23.19623402057282
35	23.066940886418514	26.855736749038222	27.020046773692652	23.057275590850608
36	23.856801516025378	26.597229704463782	26.87023469239008	22.675734087120755
37	23.660168207832374	26.070471096012827	26.930048611683283	23.339312084471516
38	23.419961845849627	26.584633130731838	26.833791774675024	23.161613248743514
39	22.99809546143072	26.359241607037603	27.3486668230015	23.293996108530177
40	22.9153066592302	26.992556137938028	27.410619783117106	22.681517419714666
41	23.10544362122182	25.74272250777977	27.610501264410797	23.541332606587613
42	22.687934542181885	26.791010958226906	27.458074799880848	23.062979699710358
43	23.216198401581938	25.92256038433018	27.285208611936802	23.576032602151084
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	49.0
2	66.0
3	242.5
4	419.0
5	419.0
6	504.0
7	589.0
8	569.5
9	550.0
10	755.5
11	961.0
12	961.0
13	1539.0
14	2117.0
15	3989.0
16	5861.0
17	6819.0
18	7777.0
19	7777.0
20	7518.5
21	7260.0
22	8387.5
23	9515.0
24	12510.5
25	15506.0
26	15506.0
27	20157.5
28	24809.0
29	31280.0
30	37751.0
31	43480.0
32	49209.0
33	49209.0
34	58187.5
35	67166.0
36	72354.5
37	77543.0
38	81570.5
39	85598.0
40	85598.0
41	90202.0
42	94806.0
43	97114.0
44	99422.0
45	98254.5
46	97087.0
47	97087.0
48	95570.5
49	94054.0
50	94005.5
51	93957.0
52	91911.0
53	89865.0
54	89865.0
55	85059.0
56	80253.0
57	70827.5
58	61402.0
59	55549.5
60	49697.0
61	49697.0
62	44240.5
63	38784.0
64	31773.0
65	24762.0
66	20820.5
67	16879.0
68	16879.0
69	14164.5
70	11450.0
71	9387.5
72	7325.0
73	5721.0
74	4117.0
75	4117.0
76	3173.0
77	2229.0
78	1709.5
79	1190.0
80	1004.5
81	819.0
82	819.0
83	666.5
84	514.0
85	492.5
86	471.0
87	348.5
88	226.0
89	226.0
90	193.5
91	161.0
92	100.0
93	39.0
94	24.0
95	9.0
96	9.0
97	4.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1262248.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.22425412092728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.83988880687109	47.4044383318279
2	8.447791226745666	9.330459389326851
3	2.0305666166329406	3.36409580539227
4	0.9105207090466874	2.011313080750446
5	0.5127594933642101	1.415838028223153
6	0.3372827792672889	1.1175713947721628
7	0.2378834597640316	0.9195855643221982
8	0.17535874227658277	0.774724459664656
9	0.1488146002353563	0.7396357770271369
>10	1.1031166413743523	12.327329706775911
>50	0.14423628824936585	5.569764560240718
>100	0.10248978248121286	10.590304630148507
>500	0.007839157802095636	2.9918202941822036
>1k	0.0014516958892769694	1.4431189773459592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4632	0.3669643366438291	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3169	0.2510600135631033	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1759	0.13935454839302577	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1487	0.11780569270064202	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1312	0.10394153922208631	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.922373416317554E-5	0.0	0.0
2	7.922373416317554E-5	0.0	1.5844746832635107E-4	7.922373416317554E-5	0.0
3	7.922373416317554E-5	0.0	1.5844746832635107E-4	7.922373416317554E-5	0.0
4	7.922373416317554E-5	0.0	1.5844746832635107E-4	1.5844746832635107E-4	0.0
5	1.5844746832635107E-4	0.0	5.545661391422288E-4	3.1689493665270214E-4	0.0
6	1.5844746832635107E-4	0.0	0.0011091322782844576	3.1689493665270214E-4	0.0
7	1.5844746832635107E-4	0.0	0.002772830695711144	3.1689493665270214E-4	0.0
8	1.5844746832635107E-4	0.0	0.0034858443031797236	3.1689493665270214E-4	0.0
9	1.5844746832635107E-4	0.0	0.004198857910648304	3.961186708158777E-4	0.0
10	4.7534240497905324E-4	0.0	0.004832647783953708	4.7534240497905324E-4	0.0
11	4.7534240497905324E-4	0.0	0.007605478479664852	5.545661391422288E-4	0.0
12	4.7534240497905324E-4	0.0	0.00816004461880708	5.545661391422288E-4	0.0
13	4.7534240497905324E-4	0.0	0.009031505694602012	6.337898733054043E-4	0.0
14	4.7534240497905324E-4	0.0	0.009665295567907416	6.337898733054043E-4	0.0
15	4.7534240497905324E-4	0.0	0.009902966770396943	7.922373416317554E-4	0.0
16	4.7534240497905324E-4	0.0	0.010140637972886469	8.714610757949309E-4	0.0
17	4.7534240497905324E-4	0.0	0.010378309175375995	0.001029908544121282	0.0
18	4.7534240497905324E-4	0.0	0.010536756643702346	0.0011883560124476332	0.0
19	4.7534240497905324E-4	0.0	0.010695204112028699	0.0011883560124476332	0.0
20	4.7534240497905324E-4	0.0	0.011091322782844576	0.0012675797466108086	0.0
21	4.7534240497905324E-4	0.0	0.01117054651700775	0.0014260272149371597	0.0
22	5.545661391422288E-4	0.0	0.011487441453660454	0.0018221458857530374	0.0
23	5.545661391422288E-4	0.0	0.011487441453660454	0.0021390408224057395	0.0
24	5.545661391422288E-4	0.0	0.011566665187823628	0.0026936069615479683	0.0
25	5.545661391422288E-4	0.0	0.01172511265614998	0.003089725632363846	0.0
26	5.545661391422288E-4	0.0	0.01172511265614998	0.003961186708158777	0.0
27	5.545661391422288E-4	0.0	0.01172511265614998	0.004594976581464181	0.0
28	6.337898733054043E-4	0.0	0.01172511265614998	0.012121231326965859	0.0
29	6.337898733054043E-4	0.0	0.01172511265614998	0.028124425627927316	0.0
30	6.337898733054043E-4	0.0	0.01172511265614998	0.05450592910426477	0.0
31	6.337898733054043E-4	0.0	0.01172511265614998	0.12446048637034877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1305	0.0	25.37548	1
GTATCAA	3315	0.0	22.378582	1
TTAACGG	225	0.0	21.377777	35
AACGGCC	240	0.0	20.8125	37
TCGCGCT	45	0.0038260126	20.555557	23
TAACGGC	255	0.0	18.862745	36
CATAATA	290	0.0	18.5	2
GCGTAAT	50	0.007035255	18.5	1
TTCTGCG	165	3.6379788E-12	17.939394	18
TAGGACC	320	0.0	17.921875	4
CGAACTA	365	0.0	17.232876	29
AATACGC	65	0.00158005	17.076923	5
ATAATAT	355	0.0	16.676056	3
GCGAACT	380	0.0	16.552631	28
TGCGGGT	195	1.8189894E-12	16.128206	21
ACGTATA	70	0.0025926824	15.857143	29
GTACCCT	235	0.0	15.744681	1
AAAGCGA	400	0.0	15.725	25
TAACCGT	240	0.0	15.416667	7
CATGCTA	410	0.0	15.341464	4
>>END_MODULE