FastQCFastQC Report
Fri 10 Feb 2017
ERR1633434.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633434.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302557
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT156551.2018667897067077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT110440.8478707649646042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59970.46040211675957365No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA42520.3264348508357024No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA38930.29887367692930134No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG31470.2416017110959444No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA28800.22110356782850962No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC27580.211737375024663No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.20713105069490242No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA26390.2026014984373045No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC25690.19722745338591707No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT25340.1945404308602234No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG23840.18302462003582184No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC23180.17795766327308515No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG23160.17780411912875982No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT23110.17742025876794643No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC21270.16329419749001386No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT20890.16037685874783214No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG20880.16030008667566947No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA20880.16030008667566947No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG20070.15408154883049263No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG19810.15208547495426303No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT19750.15162484252128697No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT19410.14901459206775597No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC19300.14817009927396652No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG19190.14732560648017706No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG18940.14540630467611015No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT18910.1451759884596221No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC18710.14364054701636858No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT18420.14141415692365095No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC18080.13880390647011992No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG18040.1384968181814692No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC17770.13642397223307692No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC17430.13381372177954592No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC17400.1335834055630579No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG17270.13258536862494308No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA17270.13258536862494308No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16400.1259061983467902No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16190.12429398483137398No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA16140.12391012447056059No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG16030.12306563167677116No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC15680.12037860915107745No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT15370.11799867491403447No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA15340.11776835869754644No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA15180.11654000554294361No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT15120.11607937310996753No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA15090.11584905689347952No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG15020.11531165238834078No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG14990.11508133617185275No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG14840.1139297550894126No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC14750.1132388064399485No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG14710.1129317181512978No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG14540.11162659292453228No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA14480.11116596049155622No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT14460.11101241634723087No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14400.11055178391425481No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC14360.11024469562560409No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA14310.10986083526479072No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG14220.10916988661532662No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA14130.10847893796586254No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA14020.10763444517207309No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC14020.10763444517207309No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG13920.1068667244504463No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC13740.10548482715151813No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT13710.10525451093503009No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC13710.10525451093503009No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13510.10371906949177656No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC13470.10341198120312585No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT13250.10172299561554696No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC13140.1008785028217575No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT13140.1008785028217575No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG13080.10041787038878144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG303.5997393E-430.8333342
GGTATCA29650.023.6475541
GTATCAA79400.023.4861471
CCGCTCT5100.021.76470628
CGCTCTC4950.021.67676729
CCTAGAC450.003826076620.5555573
GCTCTCT5350.020.05607430
TGCACTG1202.382876E-1020.0416685
GCCGCTC5600.019.8214327
TTCGCCG5500.019.5090924
TTGCGGT1900.019.47368435
ACTAGTG1457.2759576E-1219.137938
GGCGAGT4650.019.09677319
GCCGGCA5950.018.96638715
GGACGTA2050.018.95121827
TTCTGCG4250.018.71764618
CTAGATA609.237715E-418.53
CCGGCAG6200.018.516
ACTGTCC801.6169408E-518.58
GCAGCTT6100.018.49999819
Produced by FastQC (version 0.11.5)