FastQCFastQC Report
Fri 10 Feb 2017
ERR1633433.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633433.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317885
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT208121.579196970904138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT150541.1422847972319283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85780.6508913903716941No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA79890.6061985681603478No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA74880.5681831115765033No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG62610.47507938856577014No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA57740.43812624014993723No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT54620.4144519438342496No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC46440.35238279516042753No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT45510.345326033758636No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT42010.3187683295583454No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC38120.2892513383185938No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC35680.2707368245332483No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35560.2698262746749527No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG35450.26899160397151495No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT35140.26663935017091783No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC35080.26618407524176996No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA34110.25882379722054655No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC33560.254650443703358No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC32930.2498700569473057No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA32360.24554494512040126No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA31870.24182686653236055No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA30680.23279724710426175No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA29820.22627163978647608No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC29770.22589224401218616No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA28140.21352394177033657No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG28030.21268927106689886No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG27620.20957822571772197No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA27530.20889531332400021No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG26980.20472195980681165No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC26660.20229382685135652No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC26300.1995621772764695No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC26190.19872750657303181No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT26100.19804459417931006No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC25130.19068431615808665No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG24900.18893909559635325No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT24850.1885596998220634No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG24750.18780090827348364No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG24560.18635920433118217No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG24040.18241348827856754No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG23730.18006123447797037No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT23690.17975771785853847No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT23500.178316013916237No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA23470.1780883764516631No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG23400.17755722236765728No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG23360.17725370574822538No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT23250.17641903504478768No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA22970.17429441870876441No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA22720.1723974398373151No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21480.1629884246349264No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT21430.16260902886063655No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA21310.16169847900234088No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG20860.15828391703373207No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG20810.1579045212594422No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG20690.15699397140114654No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC20450.15517287168455518No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC19820.15039248492850285No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG19770.150013089154213No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA19370.1469779229598941No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT19160.14538446070787664No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC18400.13961764493867068No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC18100.1373412702929315No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC18070.13711363282835756No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA17760.1347613790277604No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG17750.1346854998729024No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA17750.1346854998729024No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG17390.13195385029801537No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA17260.13096742128486172No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT17120.1299051131168501No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG17010.1290704424134124No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA16960.12869104663912254No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG16940.1285392883294066No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC16600.1259593970642355No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG16570.12573175959966157No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG16500.12520060551565576No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT16460.12489708889622388No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA16400.12444181396707604No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16010.12148252692761508No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG16000.1214066477727571No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG15920.12079961453389332No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG15750.11950966890130778No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG15490.11753681087500047No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC15420.11700565679099466No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT15220.1154880736938352No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC14850.11268054496409018No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG14700.11154235764122059No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG14560.11048004947320897No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA14340.10881070806633357No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG14330.1087348289114756No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA14300.10850719144690167No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT14260.10820367482746976No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC14240.10805191651775381No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG14110.10706548750460017No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA13970.10600317933658855No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA13750.10433383792971315No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA13670.10372680469084936No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC13530.10266449652283773No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA13490.10236097990340583No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13470.10220922159368988No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG13380.10152630919996813No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA13360.10137455089025219No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGGT250.005496633729.5999984
GGACCCA250.005496633729.59999834
GGTATCA46700.023.9271951
GAATCAA400.001931336123.1250022
CCACTAG400.001931336123.1250023
ACAACGC903.8289727E-922.611113
GTATCAA116400.022.4574741
CCGCCGC4500.021.78888932
CGCCGCC4350.021.68965533
CATAATA603.7268208E-521.5833322
ATACGGG603.7268208E-521.5833323
TAGACCG957.1468094E-921.4210535
CGCTCTC5700.021.42105329
TAATATA705.10286E-621.1428574
TCCGCCG4650.021.08602131
GCCGGCA5800.021.05172315
GCTCTCT6000.020.65833330
GCCGTCC5200.020.63461527
GGCAGCT6100.020.62295218
ATAAATT450.003826101320.55555521
Produced by FastQC (version 0.11.5)