FastQCFastQC Report
Fri 10 Feb 2017
ERR1633432.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633432.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287455
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT159431.2383345437316255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT113610.8824386095047981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63140.4904249080550388No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA59390.461297676423642No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA55310.42960724840868225No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG46260.35931352940491124No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA42940.33352622033391455No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT34640.26905794765642294No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT34430.26742682268506474No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT30720.23861028152440278No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC30280.23519268634631887No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC28840.22400782939986252No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.21787169260284825No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC27940.21701729380832727No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG26710.20746356183322912No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT26530.20606545471492207No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC25610.1989195738880194No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC25270.19627870488677274No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC24560.19076394903122829No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA23850.1852491931756838No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC23830.18509384794031636No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC23450.1821422884683348No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA23440.1820646158506511No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC23160.1798897825555068No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG23100.17942374684940446No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA22970.17841400281951603No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC22900.17787029449572994No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT22180.17227786602250175No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG21500.16699612802000846No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG21460.16668543754927356No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA21130.16412224116571064No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21110.1639668959303432No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG21090.16381155069497574No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT21050.16350086022424085No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG21000.1631124971358222No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG20830.1617920626351989No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA20400.15845214007479874No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA19970.15511221751439855No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT19660.1527043663662031No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC19610.15231600327778447No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA19290.14983047951190526No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC19110.14843237239359822No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC18650.1448594319801469No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG18530.14392736056794217No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA18520.14384968795025846No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG18460.1433836522441561No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT18270.14190787250816533No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC18230.14159718203743044No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG18210.141441836802063No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG18100.140587438007542No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT18070.14035442015449084No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC18050.1401990749191234No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG17850.13864562256544888No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT17830.13849027733008143No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG17820.13841260471239772No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG17730.13771355115324418No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC17580.13654846188798833No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG17490.1358494083288348No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG17450.1355387178580999No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA17140.13313086670990443No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT17050.1324318131507509No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16710.12979094414950426No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG16680.12955792629645307No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT16600.12893654535498328No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG16440.1276937834720437No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG16340.12691705729520641No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG16280.1264510215891041No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC16170.1255966227945831No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG15900.12349946211712255No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA15690.12186833714576432No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA15680.1217906645280806No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC15650.12155764667502941No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG15630.12140230143966196No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT15400.1196158312329363No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT15380.11946048599756885No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG15370.11938281337988511No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA15210.11814005149694554No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA14540.11293598611213597No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG14370.11161555161151263No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG14310.11114951590541029No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA14120.10967373616941951No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC14040.10905235522794972No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG13950.1083533016687962No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA13910.1080426111980613No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT13850.10757657549195895No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT13710.1064891588443868No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC13640.10594545052060073No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC13550.1052463969614472No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC13230.10276087319556801No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA13210.10260552796020055No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT13060.10144043869494469No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT13000.10097440298884233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACTC451.0801341E-732.8888931
ACTCGGT451.0801341E-732.8888934
GACTCGG451.0801341E-732.8888933
GTACAAC250.00549658529.61
AGACTCG604.3237378E-827.75000232
GGACTTA604.3237378E-827.75000224
TCGTTGC556.262744E-726.9090916
CTCGTTG556.262744E-726.9090915
CGTTGCG556.262744E-726.9090917
ACTTATG556.262744E-726.9090912
TTAAGAC556.262744E-726.9090929
CCCATCC358.869978E-426.428578
TAACAAC509.092195E-625.9000021
CGCTCGT601.3376157E-624.66666813
TTGCGGG601.3376157E-624.66666819
GCTCGTT601.3376157E-624.66666814
TATGTCA601.3376157E-624.66666815
GTTGCGG601.3376157E-624.66666818
GGGGACA451.3233333E-424.66666618
TCTTTGT1550.023.87096837
Produced by FastQC (version 0.11.5)