FastQCFastQC Report
Fri 10 Feb 2017
ERR1633431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101779
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66560.6041138921689377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43710.3967220286463982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27480.2494148100481131No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18060.16391672014079048No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16890.1532975306300084No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12680.11508660085189498No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT12170.11045772337283612No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.10455817364462383No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC11450.10392283752004713No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11340.10292445218142657No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC11040.10020158307609785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10700.030.77571
CGAGTCG754.620233E-1027.13333321
GTATCAA31500.023.8444441
GCCGGCA2050.021.65853715
TTTGCCG603.7261067E-521.5833329
CCGTCCG957.1449904E-921.42105328
TTCGCCG1900.021.42105324
GCTTCGC2250.021.37777722
CCGCTCT1850.021.028
AGGACCG1251.8189894E-1120.725
TTCGATC555.142909E-420.18181833
CGCTCTC1850.020.029
ACGTATA656.901797E-519.92307729
AAACACG759.265199E-619.73333415
AGCTTCG2550.019.58823621
GCCGCTC2100.019.3809527
GAGTCGC1153.0504452E-919.30434622
CGTCCGC1205.173206E-918.529
GCGAATC1002.874658E-718.49999833
CGCCGGC2500.017.7614
Produced by FastQC (version 0.11.5)