##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633431.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1101779
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.854243001545683	33.0	33.0	33.0	33.0	33.0
2	31.97428794703838	33.0	33.0	33.0	33.0	33.0
3	31.69627121228486	33.0	33.0	33.0	27.0	33.0
4	31.969515665119776	33.0	33.0	33.0	33.0	33.0
5	32.068703433265654	33.0	33.0	33.0	33.0	33.0
6	35.23589304207105	37.0	37.0	37.0	33.0	37.0
7	35.38485758033145	37.0	37.0	37.0	33.0	37.0
8	35.516834138243695	37.0	37.0	37.0	33.0	37.0
9	35.62744978802464	37.0	37.0	37.0	33.0	37.0
10	35.63739461362034	37.0	37.0	37.0	33.0	37.0
11	35.63794009506444	37.0	37.0	37.0	33.0	37.0
12	35.64666053718577	37.0	37.0	37.0	33.0	37.0
13	35.64460658625732	37.0	37.0	37.0	33.0	37.0
14	35.61767105744437	37.0	37.0	37.0	33.0	37.0
15	35.649014003715806	37.0	37.0	37.0	33.0	37.0
16	35.62798982373053	37.0	37.0	37.0	33.0	37.0
17	35.62201947940558	37.0	37.0	37.0	33.0	37.0
18	35.60525840481621	37.0	37.0	37.0	33.0	37.0
19	35.59304270638667	37.0	37.0	37.0	33.0	37.0
20	35.5929038400623	37.0	37.0	37.0	33.0	37.0
21	35.58374138552287	37.0	37.0	37.0	33.0	37.0
22	35.49703797222492	37.0	37.0	37.0	33.0	37.0
23	35.529079788233396	37.0	37.0	37.0	33.0	37.0
24	35.52936931998159	37.0	37.0	37.0	33.0	37.0
25	35.55360557788813	37.0	37.0	37.0	33.0	37.0
26	35.43736992627378	37.0	37.0	37.0	33.0	37.0
27	35.45428166628698	37.0	37.0	37.0	33.0	37.0
28	35.47044007918103	37.0	37.0	37.0	33.0	37.0
29	35.48048655855666	37.0	37.0	37.0	33.0	37.0
30	35.486386108284876	37.0	37.0	37.0	33.0	37.0
31	35.47538390185328	37.0	37.0	37.0	33.0	37.0
32	35.46714359231751	37.0	37.0	37.0	33.0	37.0
33	35.45956947808953	37.0	37.0	37.0	33.0	37.0
34	35.43599941549076	37.0	37.0	37.0	33.0	37.0
35	35.382114743519345	37.0	37.0	37.0	33.0	37.0
36	35.402518109348605	37.0	37.0	37.0	33.0	37.0
37	35.40621576559364	37.0	37.0	37.0	33.0	37.0
38	35.38909617990541	37.0	37.0	37.0	33.0	37.0
39	35.28027399324184	37.0	37.0	37.0	33.0	37.0
40	35.071491651229515	37.0	37.0	37.0	33.0	37.0
41	35.243304691775755	37.0	37.0	37.0	33.0	37.0
42	35.28396348087956	37.0	37.0	37.0	33.0	37.0
43	34.95011522274431	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	9.0
15	35.0
16	50.0
17	36.0
18	30.0
19	31.0
20	66.0
21	174.0
22	479.0
23	1162.0
24	2427.0
25	4437.0
26	7168.0
27	10767.0
28	15407.0
29	20434.0
30	26782.0
31	34496.0
32	44348.0
33	58695.0
34	84337.0
35	171860.0
36	618549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1476539305977	19.90480849607771	14.009615358433953	23.937922214890644
2	16.04722907225496	21.859465464489702	39.467170821008565	22.626134642246768
3	18.306756618160268	27.189572500474235	30.358266040648807	24.145404840716694
4	12.696375588933897	18.102904484474656	38.93430533709573	30.266414589495717
5	13.895799429831209	37.077127082654506	34.84637118696218	14.180702300552106
6	28.954990066065882	39.483598797944055	17.65036363916902	13.91104749682105
7	26.231122575398512	32.566059073552864	22.977384756834173	18.22543359421445
8	24.367681722015032	35.50158425600778	20.741727696752253	19.38900632522493
9	26.191005637246672	15.764776783728859	19.785819116174842	38.25839846284963
10	15.362609016871803	28.034297259250724	33.18741780338888	23.415675920488592
11	33.381104559081265	24.258948482408904	23.824378573198437	18.53556838531139
12	23.069962306415352	25.820060102797388	29.82530979443246	21.284667796354803
13	28.3414368943318	22.404493097073004	26.478449852465875	22.775620156129314
14	22.460130389125226	21.498594545730132	27.807573025080345	28.233702040064294
15	24.362417508411397	28.207199447439095	24.922511683377518	22.50787136077199
16	22.71762304418581	28.564258349451205	24.91225554308078	23.8058630632822
17	22.56005968529079	27.39106481426856	26.023367662661933	24.02550783777872
18	23.176335726130194	26.65534558200873	27.744674748747254	22.42364394311382
19	25.462456626964208	25.787839485050995	27.905777837479206	20.84392605050559
20	25.15150497513567	25.266773100594587	27.990005255137373	21.591716669132378
21	23.301133893457763	26.72931685937016	27.265812835423436	22.703736411748636
22	22.657992210779113	26.7345810729738	27.349495679260542	23.257931036986545
23	22.638206028613723	26.766075592292104	28.038744612122755	22.556973766971417
24	23.296595778282214	26.87489959420174	27.029740083991438	22.79876454352461
25	23.33380832272171	26.60252192136536	27.795138589499345	22.26853116641359
26	23.32264455938986	26.51520858538781	27.425372964995702	22.736773890226626
27	23.26210610294805	26.464563220028698	27.27661354954124	22.996717127482007
28	22.46530384042535	26.843858886400994	27.918212273060206	22.772625000113454
29	23.674802297012377	26.73349192533167	26.905668015091955	22.686037762564
30	22.92301813703111	26.970926111316334	27.70428552368488	22.401770227967678
31	23.60600447095107	26.247459790030486	27.456595197403473	22.68994054161497
32	22.11577820960465	26.484984738318662	27.843424134967176	23.555812917109513
33	22.392240186099027	26.415007002311715	28.228528588764174	22.964224222825084
34	22.483365538824028	26.328147477851726	27.66997737295773	23.518509610366507
35	22.9146680051081	27.18639581985135	27.426552874941347	22.472383300099203
36	23.162176806782487	26.65997445948779	27.47937653558472	22.698472198145
37	23.838719017153167	26.032897704530583	27.060054693364094	23.068328584952155
38	23.51669436429629	26.456757661926755	27.200010165377993	22.82653780839896
39	23.026759449944137	26.281404891543588	27.635850746837615	23.055984911674663
40	22.936723244861266	26.78214052001354	27.63385397616037	22.64728225896482
41	22.815192520460094	26.06230469086813	27.9405397997239	23.181962988947873
42	22.91539410353619	26.509127511052576	27.45169403301388	23.12378435239735
43	23.01786474420006	26.246461404691868	27.515318407775062	23.22035544333301
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	57.5
2	76.0
3	321.5
4	567.0
5	567.0
6	701.5
7	836.0
8	816.0
9	796.0
10	1081.0
11	1366.0
12	1366.0
13	2155.0
14	2944.0
15	5705.5
16	8467.0
17	9857.0
18	11247.0
19	11247.0
20	10364.0
21	9481.0
22	10072.5
23	10664.0
24	13176.0
25	15688.0
26	15688.0
27	18990.5
28	22293.0
29	28522.0
30	34751.0
31	37904.5
32	41058.0
33	41058.0
34	46665.0
35	52272.0
36	55478.0
37	58684.0
38	64592.0
39	70500.0
40	70500.0
41	75238.0
42	79976.0
43	78410.0
44	76844.0
45	79821.5
46	82799.0
47	82799.0
48	83357.5
49	83916.0
50	85636.5
51	87357.0
52	88235.5
53	89114.0
54	89114.0
55	80025.5
56	70937.0
57	63301.5
58	55666.0
59	48311.5
60	40957.0
61	40957.0
62	36332.0
63	31707.0
64	26089.0
65	20471.0
66	17462.0
67	14453.0
68	14453.0
69	12119.0
70	9785.0
71	8239.0
72	6693.0
73	5378.0
74	4063.0
75	4063.0
76	3097.5
77	2132.0
78	1732.0
79	1332.0
80	1013.0
81	694.0
82	694.0
83	573.5
84	453.0
85	403.5
86	354.0
87	276.5
88	199.0
89	199.0
90	153.0
91	107.0
92	71.0
93	35.0
94	19.5
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1101779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.81534843183628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.72969598500679	40.03564587614822
2	9.084690769945123	8.68775309120826
3	2.53996618646808	3.64348104633161
4	1.1179792751001478	2.1382627431394123
5	0.6708119756636758	1.6037554174303568
6	0.4229722936565432	1.2134740558920352
7	0.31369599340058996	1.0499638258284154
8	0.22860198683594354	0.8744546922216552
9	0.17464577517061086	0.751567373273781
>10	1.334822534735348	12.76384101844197
>50	0.20729115926650382	6.984111598947634
>100	0.16323538649859712	14.853259685594555
>500	0.009272542601609889	3.0594367393203816
>1k	0.0021249576795355995	1.726186636809954
>5k	1.9317797086687265E-4	0.6148061994117645
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6656	0.6041138921689377	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4371	0.3967220286463982	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2748	0.2494148100481131	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1806	0.16391672014079048	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1689	0.1532975306300084	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1268	0.11508660085189498	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1217	0.11045772337283612	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1152	0.10455817364462383	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1145	0.10392283752004713	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1134	0.10292445218142657	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1104	0.10020158307609785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.8152460702191637E-4	0.0	0.0
2	0.0	0.0	1.8152460702191637E-4	9.076230351095818E-5	0.0
3	0.0	0.0	1.8152460702191637E-4	9.076230351095818E-5	0.0
4	0.0	0.0	3.6304921404383273E-4	1.8152460702191637E-4	0.0
5	0.0	0.0	5.445738210657492E-4	1.8152460702191637E-4	0.0
6	0.0	0.0	0.0017244837667082056	2.722869105328746E-4	0.0
7	0.0	0.0	0.003812016747460244	4.5381151755479094E-4	0.0
8	0.0	0.0	0.005354975907146533	4.5381151755479094E-4	0.0
9	9.076230351095818E-5	0.0	0.006081074335234199	8.168607315986237E-4	0.0
10	9.076230351095818E-5	0.0	0.006716410459810905	8.168607315986237E-4	0.0
11	9.076230351095818E-5	0.0	0.010346902600249234	8.168607315986237E-4	0.0
12	9.076230351095818E-5	0.0	0.01152681254589169	8.168607315986237E-4	0.0
13	9.076230351095818E-5	0.0	0.012615960188023188	8.168607315986237E-4	0.0
14	9.076230351095818E-5	0.0	0.013160534009088938	9.076230351095819E-4	0.0
15	9.076230351095818E-5	0.0	0.01406815704419852	9.9838533862054E-4	0.0
16	9.076230351095818E-5	0.0	0.015157304686330017	9.9838533862054E-4	0.0
17	9.076230351095818E-5	0.0	0.015429591596862892	0.0013614345526643727	0.0
18	9.076230351095818E-5	0.0	0.015792640810906724	0.001452196856175331	0.0
19	9.076230351095818E-5	0.0	0.01642797693548343	0.001452196856175331	0.0
20	9.076230351095818E-5	0.0	0.016700263846016306	0.0018152460702191638	0.0
21	9.076230351095818E-5	0.0	0.017698649184636848	0.001906008373730122	0.0
22	9.076230351095818E-5	0.0	0.017970936095169723	0.0021782952842629966	0.0
23	9.076230351095818E-5	0.0	0.018243223005702594	0.002813631408839704	0.0
24	9.076230351095818E-5	0.0	0.018243223005702594	0.0032674429263944947	0.0
25	9.076230351095818E-5	0.0	0.018333985309213555	0.003448967533416411	0.0
26	9.076230351095818E-5	0.0	0.01860627221974643	0.0037212544439492855	0.0
27	9.076230351095818E-5	0.0	0.01860627221974643	0.004901164389591742	0.0
28	9.076230351095818E-5	0.0	0.018787796826768344	0.012252910973979356	0.0
29	1.8152460702191637E-4	0.0	0.018787796826768344	0.028227076391907996	0.0
30	1.8152460702191637E-4	0.0	0.018787796826768344	0.04973774232400509	0.0
31	1.8152460702191637E-4	0.0	0.018787796826768344	0.10483046055515671	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1070	0.0	30.7757	1
CGAGTCG	75	4.620233E-10	27.133333	21
GTATCAA	3150	0.0	23.844444	1
GCCGGCA	205	0.0	21.658537	15
TTTGCCG	60	3.7261067E-5	21.583332	9
CCGTCCG	95	7.1449904E-9	21.421053	28
TTCGCCG	190	0.0	21.421053	24
GCTTCGC	225	0.0	21.377777	22
CCGCTCT	185	0.0	21.0	28
AGGACCG	125	1.8189894E-11	20.72	5
TTCGATC	55	5.142909E-4	20.181818	33
CGCTCTC	185	0.0	20.0	29
ACGTATA	65	6.901797E-5	19.923077	29
AAACACG	75	9.265199E-6	19.733334	15
AGCTTCG	255	0.0	19.588236	21
GCCGCTC	210	0.0	19.38095	27
GAGTCGC	115	3.0504452E-9	19.304346	22
CGTCCGC	120	5.173206E-9	18.5	29
GCGAATC	100	2.874658E-7	18.499998	33
CGCCGGC	250	0.0	17.76	14
>>END_MODULE