FastQCFastQC Report
Fri 10 Feb 2017
ERR1633430.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633430.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552428
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT157422.8496021200952883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116802.1143026783580847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60141.0886486564765No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA32790.5935615138986438No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27790.5030519814346847No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27350.49508714257785635No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG24700.447117090371958No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT23790.43064435546351737No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG22270.40312945759447383No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC21730.39335442808836624No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA20610.37308029281643945No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16170.29270782798844375No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16120.29180273266380413No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15150.2742438833657961No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC14780.26754617796346314No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA14620.2646498729246164No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14200.2570470721976439No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG14040.25415076715879714No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC13690.24781509988632003No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC13570.245642871107185No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA12800.23170440310773532No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC12650.22898911713381656No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12590.22790300274424902No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12490.22609281209496984No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12100.21903306856278104No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12050.21812797323814145No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC11960.21649880165379018No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC11960.21649880165379018No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA11190.20256033365434045No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10670.19314734227808872No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10330.1869926940705395No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA10260.1857255606160441No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10170.18409638903169281No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT10040.18174314118762988No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10020.18138110305777405No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10010.18120008399284612No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT9910.17938989334356695No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9830.1779417408241436No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9510.17214913074645022No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT9490.1717870926165944No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9350.16925282570760353No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9260.16762365412325225No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9260.16762365412325225No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9210.16671855879861266No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC9130.16527040627918932No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9060.1640032728246939No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8990.16273613937019848No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8950.1620120631104868No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8890.16092594872091928No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8710.15766760555221676No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8600.15567639583800966No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.15531435770815383No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8550.15477130051337007No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8540.15459028144844217No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT8520.1542282433185863No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC8350.1511509192148117No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA8320.15060786202002796No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8320.15060786202002796No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT8310.15042684295510003No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8270.14970276669538835No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA8270.14970276669538835No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8160.14771155698118124No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA7880.14264302316319954No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7800.14119487064377623No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7680.1390226418646412No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC7650.13847958466985744No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.13594531776086657No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7460.135040222436227No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG7410.13413512711158737No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7340.13286799365709198No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7300.1321439173973803No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA7220.13069576487795695No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.13051474581302902No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG7200.13033372674810112No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7160.12960965048838943No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG7060.12779945983911026No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC7020.12707538357939857No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA6960.12598926918983105No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6830.12363602134576814No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6750.1221878688263448No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC6600.11947258285242603No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6560.11874850659271434No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6500.11766239220314684No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6400.11585220155386766No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG6390.11567118248893973No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6330.11458506809937223No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6300.11404201090458849No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC6290.11386099183966056No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT6240.11295589651502097No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6210.1124128393202372No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6100.11042162960603011No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6070.10987857241124635No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6020.10897347708660676No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA6020.10897347708660676No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG5940.10752532456718342No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5930.1073443055022555No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG5870.10625819111268799No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA5870.10625819111268799No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.10571513391790424No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5620.10173271448949003No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC5600.1013706763596342No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5560.10064660009992252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG200.001841108237.02
TAGCAAC200.001841108237.01
CTATTTC200.001841108237.016
TCTTCCC303.59708E-430.83333234
TAAATAA303.59708E-430.8333325
CTTCCCT250.00549390329.635
TCTTCTA250.00549390329.614
TTAGATC250.00549390329.63
CCATGGG250.00549390329.628
TTCCCTG250.00549390329.636
TGGGCAG250.00549390329.631
TGAATTC250.00549390329.629
TATACCG250.00549390329.65
CGATCAC250.00549390329.617
ATTTCCT250.00549390329.618
ACGCGGG250.00549390329.67
AAATAAG358.863542E-426.428576
CGAAAAT358.863542E-426.4285724
GTCTAAA358.863542E-426.428572
TCTAAAT358.863542E-426.428573
Produced by FastQC (version 0.11.5)