FastQCFastQC Report
Fri 10 Feb 2017
ERR1633429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431737
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT106742.472338483845489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT85351.9768979726083242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45411.0517977379747392No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA22840.529025772634729No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19840.4595390249156315No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19320.447494655310988No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17470.4046444942175445No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16150.37407032522114153No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT15670.36295244558608597No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13320.30852115987279294No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC12270.28420079817110877No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11680.2705350711196863No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.2582590790226457No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11060.2561744765910728No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10510.24343523950923826No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC10380.24042414710807739No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9480.21957812279234812No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9310.21564054042159925No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9180.21262944802043834No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9000.2084602431572925No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC8920.2066072632181166No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8900.20614401823332262No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8900.20614401823332262No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8810.2040594158017497No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8590.19896372096901588No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC8210.19016206625793017No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8120.18807746382635723No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7870.18228690151643245No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7760.17973905410006555No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7700.1783493191456836No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7540.17464335926733174No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7530.17441173677493474No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA7520.17418011428253774No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7460.17279037932815577No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7440.1723271343433618No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7400.17140064437377386No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7340.1700109094193919No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7040.16306223464748215No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6690.15495544741358744No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6690.15495544741358744No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6620.1533340899668085No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6580.15240759999722053No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6500.1505546200580446No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6460.14962813008845663No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6450.14939650759605963No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6440.14916488510366266No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6420.14870164011886866No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6320.14638541519489873No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT6150.14244783282414988No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6130.14198458783935589No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6110.14152134285456192No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA6100.14128972036216492No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6080.14082647537737095No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG6080.14082647537737095No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.13712051549901907No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5910.13688889300662208No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5900.1366572705142251No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5880.1361940255294311No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5840.13526753555984314No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5800.13434104559025517No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5790.1341094230978582No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.12646588084875746No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA5410.12530776838677252No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5330.12345478844759657No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT5210.12067531853883268No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5170.1197488285692447No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.11812747112246577No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5060.11720098115287779No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT5050.1169693586604808No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5040.1167377361680838No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC5000.11581124619849585No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4990.11557962370609884No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4960.11488475622890787No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4930.1141898887517169No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA4830.111873663827747No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4800.111178796350556No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4780.11071555136576203No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4770.11048392887336503No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4740.10978906139617406No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4670.10816770394939512No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4550.10538823404063122No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4540.10515661154823423No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4500.10423012157864625No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4470.10353525410145528No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4450.10307200911666131No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4430.10260876413186733No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4410.10214551914707333No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4330.10029253920789741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC352.38488E-531.7142858
AACAGCG352.38488E-531.7142857
CACATCG303.5957884E-430.83333436
CCCACAT303.5957884E-430.83333434
CTAGTGC303.5957884E-430.8333349
GCGTGAT303.5957884E-430.83333414
GGGATGC303.5957884E-430.83333418
GGAATTG250.00549258829.618
CTTCCCA250.00549258829.631
AAACATG250.00549258829.621
CTATTAC250.00549258829.61
ATAACAG405.9316724E-527.755
AGGGATA405.9316724E-527.751
TAAGTTC556.246537E-726.9090930
AAGTTCC556.246537E-726.9090931
ACAACGC358.86039E-426.428573
CGTGATG358.86039E-426.4285715
TTCCCTG358.86039E-426.4285736
GAAAGCT358.86039E-426.4285715
AGGCACT358.86039E-426.4285725
Produced by FastQC (version 0.11.5)